Different combinations of salts affect the growth and bacteriocin production by Lactobacillus salivarius CRL 1328

BACKGROUND: The culture medium for optimal growth of vaginal Lactobacillus salivarius CRL 1328 is different from that for optimal bacteriocin production. To simultaneously obtain high amount of biomass and bacteriocin of this microorganism, the effects of different basal culture media and salts on both responses were evaluated. The study was performed by using a complete factorial experimental design 2⁶, with central points. Sixty‐four different growth media, which resulted from the combinations of two basal culture media and two concentrations of five salts (ammonium citrate, sodium acetate, MgSO₄, MnSO₄, and K₂HPO₄) were assayed. RESULTS: Only the addition of MnSO₄ to each culture medium significantly stimulated the growth of L. salivarius. The presence of sodium acetate or MgSO₄ stimulated the bacteriocin production, while MnSO₄ and K₂HPO₄ exerted an inhibitory effect. However, the simultaneous addition of MnSO₄ and sodium acetate to both basal culture media allowed high bacteriocin levels to be reached, attenuating the inhibitory effect of Mn²⁺. CONCLUSIONS: The application of a complete experimental design contributed to simultaneous optimization of the biomass and bacteriocin production of L. salivarius CRL 1328. The results obtained are potentially applicable to the technological production of probiotic bacteria and antagonistic substance to be included in a probiotic pharmaceutical product. Copyright © 2009 Society of Chemical Industry     

Evaluation of Gene Expression Knock-Down by Chemically and Structurally Modified Gapmer Antisense Oligonucleotides

We analyzed the effect of modified nucleotides within gapmer antisense oligonucleotides on RNase H mediated gene silencing. Additionally, short hairpins were introduced into antisense oligonucleotides as structural motifs, and their influence on biological and physicochemical properties of pre-structured gapmers was investigated for the first time. The results indicate that two LNA residues in specified positions of the gap flanking regions are sufficient and favorable for efficient knock-down of the β-actin gene. Furthermore, the introduction of other modified nucleotides, i. e. glycyl-amino-LNA−T, 2′-O-propagyluridine, polyamine functionalized uridine, and UNA, in specified positions, also increases the inhibition of β-actin expression. Importantly, the presence of hairpins within the gapmers improves their silencing properties.     

Characteristics and 3D formation of PVA and PEO electrospun nanofibers with embedded urea

The behavior of electrospun polyvinyl alcohol (PVA) and polyethylene oxide (PEO) nanofibers embedded with urea is studied as a function of various process parameters. Our results show that three‐dimensional nanofiber networks can be obtained when high concentrations of urea in the solution are used during electrospinning. The nanofibers are characterized using both scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). The stability of the nanofiber as a function of electric field has also been studied. The successful formation of three‐dimensional nanofiber networks can open new trends toward applications in fertilizers containing nanofibers in the nanoagricultural field. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 39840.     

Preparation of dendritic adamantane‐based polymers/layered silicate nanocomposites

An adamantane‐based atom transfer radical initiator (Adm‐Br) was prepared by the treatment of 1‐[[N‐[2‐Hydroxy‐l,l‐bis(hydroxymethyl)ethyl]amino]carbonyl]adamantane with bromopropionyl bromide. The resulting initiator was subsequently used in the atom transfer radical polymerization (ATRP) of methyl methacrylate (MMA) to form (Adm‐p‐MMA), which was successfully used, as a macroinitiator, in further ATRP reactions with 3‐O‐methacryloyl‐1,2 5,6‐di‐O‐isopropylidene‐α‐d ‐glucofuranose (gly), a glycomonomer, to afford the Adm‐p‐MMA‐b‐gly polymer. The new Adm‐p‐MMA‐b‐gly polymer subsequently was employed to form a nanocomposite with chitosan‐modified, Nanofil clay (NC). The resulting Adm‐p‐MMA‐b‐gly/NC composite material was progressively hydrolyzed to regenerate the OH groups of the glucose units within the Adm‐p‐MMA‐b‐gly copolymer. The polymer/NC nanocomposites were characterized by X‐ray diffraction, thermal gravimetric analysis, differential scanning calorimetry, and transmission electron microscopy. POLYM. ENG. SCI., 54:2669–2675, 2014. © 2013 Society of Plastics Engineers     

Cloaking a receiving antenna or a sensor with plasmonic metamaterials

In this contribution, we discuss the possibility of applying the scattering-cancellation-based plasmonic cloaking technique to reduce the scattering from a sensing device, lowering its disturbance on sensing without affecting its ability to measure and “sense” the external world. The plasmonic cloak, while allowing the external fields to penetrate into the cover, may be able to suppress the scattering without necessarily shielding its interiors from the impinging light, which may be of great use if we wish to “sense” the external world, or receive an external signal, from inside the cloak. Our results may provide a unique sensing system that may receive and transmit signals, while its presence may not be perceived by the surrounding. This may be of great importance in a wide range of biological, optical, physical and engineering applications.     

The Epidermal Growth Factor Receptor and Its Ligands in Cardiovascular Disease

The epidermal growth factor receptor (EGFR) family and its ligands serve as a switchboard for the regulation of multiple cellular processes. While it is clear that EGFR activity is essential for normal cardiac development, its function in the vasculature and its role in cardiovascular disease are only beginning to be elucidated. In the blood vessel, endothelial cells and smooth muscle cells are both a source and a target of EGF-like ligands. Activation of EGFR has been implicated in blood pressure regulation, endothelial dysfunction, neointimal hyperplasia, atherogenesis, and cardiac remodeling. Furthermore, increased circulating EGF-like ligands may mediate accelerated vascular disease associated with chronic inflammation. Although EGFR inhibitors are currently being used clinically for the treatment of cancer, additional studies are necessary to determine whether abrogation of EGFR signaling is a potential strategy for the treatment of cardiovascular disease.     

Enhancement of immune response in mice fed with Streptococcus thermophilus and Lactobacillus acidophilus

Swiss mice, fed for 8 consecutive d with 50 micrograms/d of viable cultures of Lactobacillus acidophilus and Streptococcus thermophilus, showed significant variation in their immune system. In order to study this phenomenon assays for macrophage and lymphocyte function were carried out. Both lactic acid bacteria enhanced significantly the enzymatic and phagocytic activity of peritoneal macrophages as checked against the controls and also accelerated the phagocytic function of the reticuloendothelial system as revealed by the carbon clearance test. On the 2nd d (100 micrograms), L. acidophilus reached a peak of K = .271, which remained high. Streptococcus thermophilus was effective only on the 2nd d and then decreased. The lymphocytic activity studied by immunoglobulin secreting cells was assayed by Jerne's method of plaque-forming cells (PFC). This activity also was increased by the two microorganisms. Streptococcus thermophilus proved more effective than L. acidophilus. Lactobacillus acidophilus and S. thermophilus activated macrophages and lymphocytes and produced the same increase in the immune response of mice whether administered orally or intraperitoneally.     

Experimental and theoretical study of density jumps on smooth and rough beds

Hydraulic jumps in density currents are technically referred to density jumps. These jumps significantly influence the dynamic and quality characteristics of the gravity currents. The density jump is studied theoretically and experimentally in this study by considering the bed roughness. Experiments were performed in a rectangular laboratory flume (0.4 m width; 0.9 m depth; 8.3 m length). Four rough beds comprised of closely packed gravel particles glued onto the horizontal part of the bed were examined. For both smooth and rough beds, a simple relationship was obtained for estimating the conjugate depth ratio as a function of the relative roughness and the upstream densimetric Froude number. The conjugate depth ratio was found to decrease with increasing relative roughness. The results also indicated that, if the entrainment ratio is specified, the minimum value of the upstream densimetric Froude number increases with increasing relative bed roughness. An equation for calculating the maximum possible value of the relative roughness was also determined. The spatial development of the density current for smooth beds was analysed in both super‐critical and sub‐critical flow regimes. Good similarity collapses of velocity and concentration profiles were obtained for the super‐critical section just upstream of the jump. The concentration distributions located just downstream of the jump, however, exhibited a large scattering of measured data, especially near the bed. It was found that this scattering decreases with the distance from the end of the jump. The results of the experimental runs also indicated that, at a distance about nine times the post‐jump current thickness from the end of the jump, the non‐dimensional vertical profile of mean velocity has a shape similar to that at the pre‐jump section. A new reliable relationship was also proposed for calculating the local velocity inside both the wall and jet regions.     

Parametric estimation of change-points for actual event data in recurrent events models

Time to event data have long been important in many applied fields. Many models and analysis methods have been developed for this type of data in which each sample unit experiences at most a single end-of-life event.In contrast, many applications involve repeated events, where a subject or sampling unit experiences more than one event. There is growing interest in the analysis of recurrent events data, also called repeated events and recurrence data. This type of data arises in many fields. For example, the repair history of manufactured items can be modeled as recurrent events. In medical studies, the times of recurrent disease episodes in patients can also be modeled as recurrent events. In this paper we focus on medical applications (e.g. seizures, heart attacks, cancerous tumors, etc.). However, our proposed methodologies can be applied to other areas as well.For analyzing recurrence data, the first and perhaps most important step is to model the expected number of events, and sometimes this can be facilitated by modeling the cumulative intensity function or its derivative, the intensity rate function. One particular recurrent events scenario involves patients experiencing events according to a common intensity rate, and then a treatment may be applied. Assuming the treatment to be effective, the patients would be expected to follow a different intensity rate after receiving the treatment. Further, the treatment might be effective for a limited amount of time, so that a third rate would govern arrivals of the recurrent events after the effects of the treatment wore out. In this paper we model the intensity rate for such scenarios. In particular we allow models for the intensity rate, post-treatment, to be piecewise constant. Two estimators of the location of this change are proposed. Properties of the estimators are discussed. An example is studied for illustrative purposes.