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排序方式: 共有1405条查询结果,搜索用时 15 毫秒
811.
Koichi Fujisawa Taro Takami Shoki Okubo Yuto Nishimura Yusaku Yamada Keisuke Kondo Toshihiko Matsumoto Naoki Yamamoto Isao Sakaida 《International journal of molecular sciences》2021,22(18)
Among lifestyle-related diseases, fatty liver is the most common liver disease. To date, mammalian models have been used to develop methods for inhibiting fatty liver progression; however, new, more efficient models are expected. This study investigated the creation of a new model to produce fatty liver more efficiently than the high-fat diet medaka model that has been used to date. We compared the GAN (Gubra-Amylin nonalcoholic steatohepatitis) diet, which has been used in recent years to induce fatty liver in mice, and the high-fat diet (HFD). Following administration of the diets for three months, enlarged livers and pronounced fat accumulation was noted. The GAN group had large fat vacuoles and lesions, including ballooning, compared to the HFD group. The GAN group had a higher incidence of lesions. When fenofibrate was administered to the fatty liver model created via GAN administration and liver steatosis was assessed, a reduction in liver fat deposition was observed, and this model was shown to be useful in drug evaluations involving fatty liver. The medaka fatty liver model administered with GAN will be useful in future fatty liver research. 相似文献
812.
Naoki Nishino Mayumi Taniguchi Ei Sakaguchi 《Journal of the science of food and agriculture》2001,81(11):1055-1059
The antinutritional activities of trypsin inhibitors (TIs) were compared between winged beans (Psophocarpus tetragonolobus) and soya beans (Glycine max). The inhibitors of the two beans were isolated by trypsin‐bound Sepharose 4B, and 50 mg of lyophilised powders were intubated intragastrically into 24 h fasted rats. The activities of trypsin and chymotrypsin were compared after 30, 60 and 180 min in the washings of the upper, middle and lower parts of the small intestine. The elution profiles of TI and non‐TI compounds in the affinity chromatography were similar in the two beans, and the antitryptic activities were concentrated 5.5 and 6.2 times (based on specific activity) for winged beans and soya beans respectively. Regardless of the TI fed to rats, trypsin activity in the upper intestine was suppressed to almost undetectable levels at 30 and 60 min after intubation. The activities in the middle and lower intestines were also substantially lowered when rats were fed winged bean TI, and significant differences were detected at 30 and 60 min after intubation when compared with rats fed soya bean TI. However, at 180 min after feeding, no differences were found in the trypsin activity in any gut segments. Similar inhibitory properties of isolated TIs were observed in chymotrypsin activities in the small intestine. The results suggest that winged bean TI may have greater inhibitory activity on the intestinal proteinase compared with soya bean TI. © 2001 Society of Chemical Industry 相似文献
813.
An Fe2O3 (10 wt%)/Al2O3 (90 wt%) catalyst prepared by a coprecipitation method was found to be effective for dehydrogenation of ethylbenzene to produce
styrene in the presence of CO2 instead of steam used in commercial processes. The dehydrogenation of ethylbenzene over the catalyst in the presence of CO2 was considered to proceed both via a one-step pathway and via a two-step pathway. CO2 was found to suppress the deactivation of the catalyst during the dehydrogenation of ethylbenzene.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
814.
Enhancing the elevated temperature performance of Li/LiMn2O4 cells by reducing LiMn2O4 surface area 总被引:1,自引:0,他引:1
Lithium-rich spinels were obtained with the same structure but different surface area by two different synthesis routes, namely the “once-annealed” and the “twice-annealed” methods. The elevated temperature performance of Li/Li1+xMn2O4 cell is significantly improved using a spinel cathode with a small surface area: the cell at 50°C lost 5% of the initial capacity over the first 100 cycles based on a spinel cathode with the small surface area of 1.2 m2/g compared to 8% based on a large one of 6.2 m2/g. Also the mechanism responsible for the reaction of LiMn2O4 with LiOH to form lithium-rich spinel has been investigated. 相似文献
815.
Mitsuhiro Tomita Noriyoshi Kuroyanagi Kohei Ohtake Satoru Ozawa Naoki Suehiro 《International Journal of Communication Systems》2005,18(6):585-601
This paper proposes a sequential detection technique for a multi‐user receiver that is constructed over a CDMA system. In this system, the transmitter transmits a symbol made by spreading the spectrum with an enveloped sequence protected by guard sequences, and a receiver de‐modulates the core‐sequence part of the received symbol with either a de‐correlating detector or an MMSE detector. The advantage is that performance is improved without reducing the number of the active users. This sequential detection system estimates the best user signal from all of the soft outputs, which are obtained by solving a de‐correlating system of equations. Once detected, the best user component is removed from the received symbol. The resultant symbol composed of the remaining user signals is then sequentially detected by repeating the method stated above. A computer simulation of this system reveals a remarkable improvement in the bit‐error rate performance compared to conventional systems. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献
816.
Akiyoshi Kasuga Takeshi Okamoto Shohei Udagawa Chinatsu Mori Takafumi Mie Takaaki Furukawa Yuto Yamada Tsuyoshi Takeda Masato Matsuyama Takashi Sasaki Masato Ozaka Arisa Ueki Naoki Sasahira 《International journal of molecular sciences》2022,23(3)
Hereditary pancreatic cancers are caused by several inherited genes. Familial pancreatic cancer is defined as pancreatic cancer arising in a patient with at least two first-degree relatives with pancreatic cancer in the absence of an identified genetic cause. Hereditary pancreatic cancer syndromes and familial pancreatic cancers account for about 10% of pancreatic cancer cases. Germline mutations in BRCA1, BRCA2, ATM, PALB2, CDKN2A, STK11, and TP53 and mismatch repair genes (MLH1, MSH2, MSH6, PMS2, and EPCAM) are among the well-known inherited susceptibility genes. Currently available targeted medications include poly (ADP-ribose) polymerase inhibitors (PARP) for cases with mutant BRCA and immune checkpoint inhibitors for cases with mismatch repair deficiency. Loss of heterozygosity of hereditary pancreatic cancer susceptibility genes such as BRCA1/2 plays a key role in carcinogenesis and sensitivity to PARP inhibitors. Signature 3 identified by whole genome sequencing is also associated with homologous recombination deficiency and sensitivity to targeted therapies. In this review, we summarize molecular features and treatments of hereditary pancreatic cancer syndromes and surveillance procedures for unaffected high-risk cases. We also review transgenic murine models to gain a better understanding of carcinogenesis in hereditary pancreatic cancer. 相似文献
817.
Nagata Ryuji Sato Shun Kilua Aldrine Fukuma Naoki Nakayama Yasunori Kitazono Eiichi Aoyama Toshiaki Han Kyu-Ho Fukushima Michihiro 《Food science and biotechnology》2021,30(11):1417-1425
Food Science and Biotechnology - BARLEYmax, a barley variety, and cocoa polyphenols (CPPs) have been reported to affect bacterial metabolites in the colon. This study aimed to evaluate the combined... 相似文献
818.
Naoki Kawahara Yuki Yamada Hiroshi Kobayashi 《International journal of molecular sciences》2021,22(11)
Background: Ovarian clear cell carcinoma (OCCC) is resistant to platinum chemotherapy and is characterized by poor prognosis. Today, the use of poly (ADP-ribose) polymerase (PARP) inhibitor, which is based on synthetic lethality strategy and characterized by cancer selectivity, is widely used for new types of molecular-targeted treatment of relapsed platinum-sensitive ovarian cancer. However, it is less effective against OCCC. Methods: We conducted siRNA screening to identify synthetic lethal candidates for the ARID1A mutation; as a result, we identified Cyclin-E1 (CCNE1) as a potential target that affects cell viability. To further clarify the effects of CCNE1, human OCCC cell lines, namely TOV-21G and KOC7c (ARID1A mutant lines), and RMG-I and ES2 (ARID1A wild type lines) were transfected with siRNA targeting CCNE1 or a control vector. Results: Loss of CCNE1 reduced proliferation of the TOV-21G and KOC7c cells but not of the RMG-I and ES2 cells. Furthermore, in vivo interference of CCNE1 effectively inhibited tumor cell proliferation in a xenograft mouse model. Conclusion: This study showed for the first time that CCNE1 is a synthetic lethal target gene to ARID1A-mutated OCCC. Targeting this gene may represent a putative, novel, anticancer strategy in OCCC treatment. 相似文献
819.
Naoki Inui Satoshi Sakai Masatoshi Kitagawa 《International journal of molecular sciences》2021,22(11)
Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease. During the past decade, novel pathogenic mechanisms of IPF have been elucidated that have shifted the concept of IPF from an inflammatory-driven to an epithelial-driven disease. Dysregulated repair responses induced by recurrent epithelial cell damage and excessive extracellular matrix accumulation result in pulmonary fibrosis. Although there is currently no curative therapy for IPF, two medications, pirfenidone and nintedanib, have been introduced based on understanding the pathogenesis of the disease. In this review, we discuss advances in understanding IPF pathogenesis, highlighting epithelial–mesenchymal transition (EMT), the ubiquitin-proteasome system, and endothelial cells. TGF-β is a central regulator involved in EMT and pulmonary fibrosis. HECT-, RING finger-, and U-box-type E3 ubiquitin ligases regulate TGF-β-Smad pathway-mediated EMT via the ubiquitin-proteasome pathway. p27 degradation mediated by the SCF-type E3 ligase, Skp2, contributes to the progression of pulmonary fibrosis by promotion of either mesenchymal fibroblast proliferation, EMT, or both. In addition to fibroblasts as key effector cells in myofibroblast differentiation and extracellular matrix deposition, endothelial cells also play a role in the processes of IPF. Endothelial cells can transform into myofibroblasts; therefore, endothelial–mesenchymal transition can be another source of myofibroblasts. 相似文献
820.
Kotaro Sugimoto Naoki Ichikawa-Tomikawa Keisuke Nishiura Yasuto Kunii Yasuteru Sano Fumitaka Shimizu Akiyoshi Kakita Takashi Kanda Tetsuya Imura Hideki Chiba 《International journal of molecular sciences》2021,22(1)
We previously reported that site-selective claudin-5 (CLDN5) breakdown and protein kinase A (PKA) activation are observed in brain microvessels of schizophrenia, but the underlying molecular basis remains unknown. The 5-HT1 receptors decline the intracellular cAMP levels and inactivate the major downstream PKA, and the 5-HT1A receptor is a promising target for schizophrenia. Therefore, we elucidated the involvement of serotonin/5-HT1A signaling in the endothelial CLDN5 expression. We demonstrate, by immunohistochemistry using post-mortem human brain tissue, that the 5-HT1A receptor is expressed in brain microvascular endothelial cells (BMVECs) and mural cells of the normal prefrontal cortex (PFC) gray matter. We also show that PKA is aberrantly activated not only in BMVECs but also in mural cells of the schizophrenic PFC. We subsequently revealed that the endothelial cell–pericyte tube-like structure was formed in a novel two-dimensional co-culture of human primary BMVECs and a human brain-derived pericyte cell line, in both of which the 5-HT1A receptor was expressed. Furthermore, we disclose that the serotonin/5-HT1A signaling enhances endothelial CLDN5 expression in BMVECs under two-dimensional co-culture conditions. Our findings provide novel insights into the physiological and pathological significance of serotonin/5-HT1A signaling in the region-specific regulation of the blood-brain barrier. 相似文献