Optimum short-time polynomial regression for signal analysis

Sādhanā - We propose a short-time polynomial regression (STPR) for time-varying signal analysis. The advantage of using polynomials is that the notion of a spectrum is not needed and the...     

A new approach to integrate PLZT thin films with micro-cantilevers

In the present work, we report the preparation of PLZT thin films in pure perovskite phase by RF magnetron sputtering without external substrate heating and their integration with micro-cantilevers. The ‘lift-off’ process for patterning different layers of a micro-cantilever including PLZT, Pt/Ti and Au/Cr was employed. The basic requirement of lift-off process is that the deposition temperature should not exceed 200°C otherwise photoresist will burn out. Therefore, one of the aims of the present work was to prepare PLZT film at lower deposition temperatures, which can be subsequently annealed to form pure perovskite phase. This also strongly favours the incorporation of ‘lift-off’ process for patterning in the complete process flow. As no external substrate heating was required in the deposition of PLZT film, this objective has been successfully accomplished in the present work. The ‘lift-off’ process has been successfully adopted for patterning the composite layers of PLZT/Pt/Ti and Au/Cr using thick positive photo-resist (STR-1045). Different types of cantilever beams incorporating PLZT films have been successfully fabricated using ‘lift-off’ process and bulk micromachining technology. The proposed process can be advantageously applied for the fabrication of various MEMS devices.     

Interference effects from coexisting fatty acids on elaidic acid separation by fractionating crystallization: A model study

A multi‐stage temperature‐programmed fractionating crystallization process was carried out to examine the effects of the presence of stearic acid (SA), oleic acid (OA), and linoleic acid (LA) on the separation of elaidic acid (EA). The results showed that the efficiency of fractionating crystallization of EA depended largely on the crystallization temperature, initial concentration of EA and presence of SA. The content of SA plays very important role for the fractionating performance. It was a characteristic observation that only when SA <2%, substantial crystallization of EA (>50% in stepwise crystal fractions) were obtained regardless of the initial concentration of SA. In general, SA induced crystallization of EA in earlier stage but delayed further crystallization of EA in later stage; the crystallization of EA was independent from co‐existing OA and LA. After reduction of EA content in solution to certain extent (7–10%, at ?20°C), further reduction of EA content requires much lower crystallization temperatures (trans‐fatty acids (TFA) from partially hydrogenated soybean oil (PHSO) is of high commercial interest. One of the strategies is to selectively release TFAs as free fatty acids from PHSO enzymatically. However, all commercially available enzymes are far away from qualified to selectively release TFAs, where there are always substantial non‐trans FAs hydrolyzed simultaneously. Therefore, developing post‐processing technology is requisite in order to recover those non‐trans fatty acids. Thus, this model system was designed based on FA composition characteristic of PHSO, which aimed to acquire some basic data and experience that lack in available literatures, so as to serve designing efficient practical process for removing trans‐fatty acid moieties from PHSO. The results from this work may be of general value to achieve a better understanding of fractionating crystallization behaviors of different FAs, relationship with individual molecular feature and property, and their interference effects, which might contribute to the design of practically feasible protocol to remove TFAs from PHSO and recover non‐trans FAs at the same time.     

Impact of Scaffold Micro and Macro Architecture on Schwann Cell Proliferation under Dynamic Conditions in a Rotating Wall Vessel Bioreactor

Over the last decade tissue engineering has emerged as a powerful alternative to regenerate lost tissues owing to trauma or tumor. Evidence shows that Schwann cell containing scaffolds have improved performance in vivo as compared to scaffolds that depend on cellularization post implantation. However, owing to limited supply of cells from the patients themselves, several approaches have been taken to enhance cell proliferation rates to produce complete and uniform cellularization of scaffolds. The most common approach is the application of a bioreactor to enhance cell proliferation rate and therefore reduce the time needed to obtain sufficiently significant number of glial cells, prior to implantation.In this study, we show the application of a rotating wall bioreactor system for studying Schwann cell proliferation on nanofibrous spiral shaped scaffolds, prepared by solvent casting and salt leaching techniques. The scaffolds were fabricated from polycaprolactone (PCL), which has ideal mechanical properties and upon degradation does not produce acidic byproducts. The spiral scaffolds were coated with aligned or random nanofibers, produced by electrospinning, to provide a substrate that mimics the native extracellular matrix and the essential contact guidance cues.At the 4 day time point, an enhanced rate of cell proliferation was observed on the open structured nanofibrous spiral scaffolds in a rotating wall bioreactor, as compared to static culture conditions. However, the cell proliferation rate on the other contemporary scaffolds architectures such as the tubular and cylindrical scaffolds show reduced cell proliferation in the bioreactor as compared to static conditions, at the same time point. Moreover, the rotating wall bioreactor does not alter the orientation or the phenotype of the Schwann cells on the aligned nanofiber containing scaffolds, wherein, the cells remain aligned along the length of the scaffolds. Therefore, these open structured spiral scaffolds pre-cultured with Schwann cells, in bioreactors could potentially shorten the time needed for grafts for peripheral nerve regeneration.     

Effects of climate change on biomass production and substitution in north-central Sweden

In this study we estimate the effects of climate change on forest production in north-central Sweden, as well as the potential climate change mitigation feedback effects of the resulting increased carbon stock and forest product use. Our results show that an average regional temperature rise of 4 °C over the next 100 years may increase annual forest production by 33% and potential annual harvest by 32%, compared to a reference case without climate change. This increased biomass production, if used to substitute fossil fuels and energy-intensive materials, can result in a significant net carbon emission reduction. We find that carbon stock in forest biomass, forest soils, and wood products also increase, but this effect is less significant than biomass substitution. A total net reduction in carbon emissions of up to 104 Tg of carbon can occur over 100 years, depending on harvest level and reference fossil fuel.     

Folic acid‐conjugated depolymerized quaternized chitosan as potential targeted gene delivery vector

Ligand‐conjugated delivery vectors that target over‐expressed cell surface receptors have a potential impact on gene therapy. In the study reported, high‐molecular‐weight chitosan was depolymerized to medium and low molecular weight and trimethylated to render the polymer soluble over a wider pH range. Folate conjugation was introduced to the quarternized derivative to improve gene transfection efficiency. Complexes of the folic acid‐conjugated trimethylated depolymerized chitosan (FTMC) with plasmid DNA (pDNA) formed core–shell nanostructured particles. Gel electrophoretic band retardation showed efficient condensation of DNA. These derivatives and their complexes with pDNA were tested for toxicity and haemocompatibility and were found to be significantly less toxic and haemocompatible than polyethyleneimine. Transfection efficiency and nuclear uptake properties were tested in the human KB oral epidermoid cell line, which over‐expresses the folate receptor in the presence of 10% serum. Among the four FTMC derivatives investigated, folic acid‐conjugated chitosan having low molecular weight and medium folate conjugation was found to be a potential vector for gene delivery applications with good transfection and nuclear uptake properties, as proved by YOYO labelling of pDNA. Copyright © 2011 Society of Chemical Industry     

A Novel Approach for Modeling Mixed Convection Film Boiling for a Vertical Flat Plate

A numerical model is developed to study mixed convection film boiling over a vertical flat plate. The integral form of conservation equations for each phase along with the appropriate interface conditions due to phase change is transformed into ordinary differential equation (ODE)-form. The length scale used in the model is based on Rayleigh–Taylor instability wave at the liquid–vapor interface. The heat transfer associated in the process is assessed and results are validated successfully for different available experimental results for natural convection and mixed convection film boiling. The mixed convection film boiling is characterized in terms of relevant nondimensional parameters for each phase.     

An efficient video watermarking based security model

Microsystem Technologies - Watermarking is defined as about hiding any valuable information set in the particular digital media by itself through modifying the particular graphic contents. In our...     

Efficient Switching and Domain Interlocking Observed in Polyaxial Ferroelectrics

We present transmission electron microscopy observations of domain wall motion in thin freestanding KNbO 3 crystals under applied electric fields. Since there is no substrate, there is no elastic clamping of 90 domains. We observe that curved and tilted 90 domain walls are the most mobile, whereas untilted 90 domain walls are resistant to field-induced motion. We explain this result in terms of two factors. First, the switching pressure on a domain wall ( P 2 m P 1 ) E is determined by the relative electrostatic energies of the neighboring polarizations P 1 and P 2 . Consequently, some 90 domain walls are immobile under certain field directions, leading to domain interlocking. Second, domain walls experiencing a high switching pressure move by a ripple mechanism, and do not move as rigid sheets. The tilted wall region in such a ripple has a polarization charge, and an associated depolarization field, which reduces the local switching barrier. An accumulation of polarization charge can result in a tilted or curved wall, as occurs at the mobile tips of 90 domain needles. Any increase in density of immobile wall configurations with cycle time represents an inherent contribution to fatigue. Uniaxial ferroelectrics, with polarizations parallel to the field, should not experience such domain interlocking.