Clearance of mucus from endotracheal tubes during intratracheal pulmonary ventilation

BACKGROUND: Intratracheal pulmonary ventilation (ITPV) is a form of tracheal gas insufflation in which all gas emerges in a cephalad direction from the tip of a reverse-thrust catheter positioned within an endotracheal tube. In vitro experiments have shown that this rapid gas flow, with 5 ml/h of normal saline added to the gas flow, continuously removes tracheal secretions from within the endotracheal tube. The authors evaluated its effectiveness to remove mucus in long-term studies in sheep. METHODS: Fourteen healthy sheep were tracheally intubated and ventilated for 3 days with ITPV or with volume-controlled ventilation. Measurements were made of the total amount of secretions within the endotracheal tubes (weight gain), the protein content within the endotracheal tubes, and the increase in resistance to constant air flow. The structure of the airways was examined grossly and histologically. Three additional sheep were ventilated for 24 h with ITPV, and Evans Blue dye was added to the saline to assess the distribution of the infused saline. RESULTS: There was significantly less mucus in endotracheal tubes of sheep ventilated with ITPV than with conventional ventilation, as shown by minimal weight gain (0.70 +/- 0.14 g vs. 2.44 +/- 0.81 g; P < 0.001), lower protein content (14.09 +/- 10.79 mg vs. 294.99 +/- 153.06 mg; P < 0.001), and lower resistance to constant air flow (6.15 +/- 0.54 cm H2O x 1(-1) x s(-1) vs. 15.34 +/- 5.28 cm H2O x 1(-1) x s(-1); P < 0.001). Results of gross and histological examinations of the tracheas of animals in both groups were similar, and the tracheas were well preserved. More than 95% of the instilled saline was recovered during ITPV. Only traces of Evans Blue dye were found near the tip of the endotracheal tubes. CONCLUSION: Intratracheal pulmonary ventilation makes it possible to keep the endotracheal tubes of sheep ventilated for 3 days free of mucus without suctioning.     

Liquid-phase mixing in external-loop airlift bioreactors

Mixing time, circulation time and downcomer linear liquid velocity were measured in two sizes of external-loop airlift bioreactors with Newtonian and non-Newtonian fluids. The mixing time increased as a result of an increase of non-Newtonian characteristics. However, the liquid circulation time was affected only slightly by non-Newtonian flow behaviour. A semi-empirical correlation for circulation time in external-loop airlift bioreactors was proposed. The proposed correlation was compared with the present experimental results and those in the literature. Reasonable agreement was obtained between the predicted and experimental values of circulation times. The downcomer liquid velocity was almost independent of the downcomer-to-rise cross-sectional area ratio. The correlation based on the concept of an eddy diffusivity predicted downcomer liquid velocities in Newtonian and non-Newtonian fluids.     

The Code Structure of HIV-1

Information of life is stored in a genome sequence and four nucleotides can be regarded as coding elements and their sequence as a certain code of life. We study what kinds of code structure the sequences of HIV-1 genes may have.     

Analytical and experimental study of a forced-convection type nocturnal radiator

An analytical model is developed to predict the nocturnal thermal performance of a forced-convection type nonselective radiator: its validity is examined by comparison of the predictions with the results of several outdoor experiments. In the experiments, the Reynolds number of a working fluid, which is water in the present study, is varied, while the inlet fluid temperature is kept constant. The presented model predicts time variations in the exit fluid temperature of the radiator accurately.     

Leptolstatin from Streptomyces sp. SAM1595, a new gap phase-specific inhibitor of the mammalian cell cycle. II. Physico-chemical properties and structure

Leptolstatin, a product from Streptomyces sp. SAM1595, is a gap phase-specific inhibitor of the mammalian cell cycle. Physico-chemical properties and spectrometric analyses showed that the structure of leptolstatin is (2Z,6E,8Z,12E,14E,22E)-19,24-dihydro xy-8,10,14,16,18,20,22-heptamethyl-17-oxo-2 , 6,8,12,14,22-tetracosahexaen-5-olide.     

A 24-b 50-ns digital image signal processor

A 50-ns digital image signal processor (DISP)-an image/video application-specific VLSI chip-is discussed. This chip integrates 538 K transistors and dissipates 1.4 W at a 40-MHz clock. It is based on a 24-b fixed-point architecture with a five-stage pipeline. The DISP features a real-time processing capability realized by an enhanced parallel architecture, video-oriented data processing functions, and an instruction cycle time that is typically 35 ns, and 50 ns at worst. This 50-ns cycle time allows the DISP to execute mor than 60-million operations per second (MOPS). High-density 1.0-μm CMOS technology allows numerous on-chip features, including specified resources optimized for image processing. This allows a flexible hardware implementation of various algorithms for picture coding. Several circuit design techniques that are intended to attain a fast instruction cycle are reviewed, including distributed instruction decoding and a hierarchical clocking circuit. The LSI has been designed by the extensive use of a cell-based design method. The processor incorporates a sophisticated testing function compatible with a cell-based design environment     

Towards realistic theories of learning

In computational learning theory continuous efforts are made to formulate models of machine learning that are more realistic than previously available models. Two of the most popular models that have been recently proposed, Valiant’s PAC learning model and Angluin’s query learning model, can be thought of as refinements of preceding models such as Gold’s classic paradigm of identification in the limit, in which the question ofhow fast the learning can take place is emphasized. A considerable amount of results have been obtained within these two frameworks, resolving the learnability questions of many important classes of functions and languages. These two particular learning models are by no means comprehensive, and many important aspects of learning are not directly addressed in these models. Aiming towards more realistic theories of learning, many new models and extensions of existing learning models that attempt to formalize such aspects have been developed recently. In this paper, we will review some of these new extensions and models in computational learning theory, concentrating in particular on those proposed and studied by researchers at Theory NEC Laboratory RWCP, and their colleagues at other institutions.     

Sexual and gonadal dysfunction in adrenal disorders

Among various diseases of the adrenals, major disorders that cause sexual and gonadal disturbances are congenital adrenal hyperplasia(CAH) and Cushing's syndrome, and the others include virilizing or feminizing adrenocortical tumors. CAH was reviewed based on the recent advances in molecular genetics. The most striking discovery was steroidogenic acute regulatory protein, mutations of which produce lipoid adrenal hyperplasia that was previously attributed to P-450scc deficiency. Reversible amenorrhea or impotence is found in patients with Cushing's syndrome. Low plasma estrogen and testosterone levels are associated with female and male patients, respectively. Elevated adrenal androgen accounts for mild virilization in female patients with ACTH-dependent subtypes. The sites of action at which hypercortisolemia suppresses the hypothalamic-pituitary-gonadal axis were discussed.     

On the Computational Complexity of Approximating Distributions by Probabilistic Automata

Machine Learning - We introduce a rigorous performance criterion for training algorithms for probabilistic automata (PAs) and hidden Markov models (HMMs), used extensively for speech recognition,...