The extraction of a glucomannan polysaccharide from konjac corms (elephant yam,Amorphophallus rivierii)

Abstract : A process for the micropropagation of Amorphophallus rivierii (elephant yam or konjac) and for the extraction and purification of the glucomannan polysaccharide from fresh konjac plant corms has been developed. This process involves extraction with 2-propanol, which has the additional benefit of extracting carotenoids as a potentially valuable side-product. Starch granules with an unusually high and homogeneous gelatinisation temperature range are normally present in the corms, particularly immature ones, and this greatly reduces the strength of gels formed using the glucomannan. Therefore, the extraction process also involves the selective hydrolysis of the starch, by α- and β-amylases that have been specially selected for an absence of contaminating β-mannanase or β-glucanase activity that would depolymerise the glucomannan and render it nonfunctional. Bacillus lichenformis α-amylase was preferred. Using this process pure glucomannan could be extracted which, when mixed with K -carrageenan, forms a gel almost twice as strong as locust bean gum-K -carrageenan gels of the same concentration.     

BETaaS: A Platform for Development and Execution of Machine-to-Machine Applications in the Internet of Things

Wireless Personal Communications - The integration of everyday objects into the Internet represents the foundation of the forthcoming Internet of Things (IoT). Smart objects will be the building...     

Gas Sensing of NiO‐SCCNT Core–Shell Heterostructures: Optimization by Radial Modulation of the Hole‐Accumulation Layer

Hierarchical core–shell (C–S) heterostructures composed of a NiO shell deposited onto stacked‐cup carbon nanotubes (SCCNTs) are synthesized by atomic layer deposition (ALD). A film of NiO particles (0.80–21.8 nm in thickness) is uniformly deposited onto the inner and outer walls of the SCCNTs. The electrical resistance of the samples is found to increase of many orders of magnitude with the increasing of the NiO thickness. The response of NiO–SCCNT sensors toward low concentrations of acetone and ethanol at 200 °C is studied. The sensing mechanism is based on the modulation of the hole‐accumulation region in the NiO shell layer upon chemisorption of the reducing gas molecules. The electrical conduction mechanism is further studied by the incorporation of an Al₂O₃ dielectric layer at NiO and SCCNT interfaces. The investigations on NiO–Al₂O₃–SCCNT, Al₂O₃–SCCNT, and NiO–SCCNT coaxial heterostructures reveal that the sensing mechanism is strictly related to the NiO shell layer. The remarkable performance of the NiO–SCCNT sensors toward acetone and ethanol benefits from the conformal coating by ALD, large surface area of the SCCNTs, and the optimized p‐NiO shell layer thickness followed by the radial modulation of the space‐charge region.     

High eEF1A1 Protein Levels Mark Aggressive Prostate Cancers and the In Vitro Targeting of eEF1A1 Reveals the eEF1A1–actin Complex as a New Potential Target for Therapy

Although the eukaryotic elongation factor eEF1A1 plays a role in various tumours, there is little information on its prognosis/therapeutic value in prostate carcinoma. In high-grade and castration-resistant prostate carcinoma (CRPC), the identification of novel therapeutic markers/targets remains a priority. The expression of eEF1A1 protein was determined in formalin-fixed, paraffin-embedded prostate cancer and hyperplasia tissue by IHC. The role of eEF1A1 was investigated in a cellular model using a DNA aptamer (GT75) we previously developed. We used the aggressive CRPC cancer PC-3 and non-tumourigenic PZHPV-7 lines. Cytotoxicity was measured by the MTS assay and eEF1A1 protein levels by in-cell Western assays. The mRNA levels of eEF1A1 were measured by qPCR and ddPCR. Higher expression of eEF1A1 was found in Gleason 7–8 compared with 4–6 tissues (Gleason ≥ 7, 87% versus Gleason ≤ 6, 54%; p = 0.033). Patients with a high expression of eEF1A1 had a worse clinical outcome. In PC-3, but not in PZHPV-7, GT75 decreased cell viability and increased autophagy and cell detachment. In PC-3 cells, but not in PZHPV-7, GT75 mainly co-localised with the fraction of eEF1A1 bound to actin. Overexpression of the eEF1A1 protein can identify aggressive forms of prostate cancer. The targeting of eEF1A1 by GT75 impaired cell viability in PC-3 cancer cells but not in PZHPV-7 non-tumourigenic cells, indicating a specific role for the protein in cancer survival. The eEF1A1–actin complexes appear to be critical for the viability of PC-3 cancer cells, suggesting that eEF1A1 may be an attractive target for therapeutic strategies in advanced forms of prostate cancer.     

Constitutive modelling of tuff masonry in direct shear

Shear behaviour of masonry has been deeply investigated for a lot of masonry types. Nevertheless, there is still a lack of knowledge on the shear response of tuff masonry, which is widely spread in the existing building inventory in seismic areas like as Italy, Turkey, and Japan. To characterize the non-linear behaviour of the unit–mortar interface within tuff masonry, a series of direct shear tests were carried out.In this paper, both experimental results and empirical formulae are presented. Classical shear strength and deformation parameters along with shearing fracture energy and dilatancy were characterized. A shear response surface including both stress–strain diagrams and the frictional strength model is proposed.     

Identification of Human Adenovirus in Respiratory Samples with Luminex Respiratory Virus Panel Fast V2 Assay and Real-Time Polymerase Chain Reaction

In order to compare the last version of the Respiratory Virus Panel (RVP) Fast assay for human Adenovirus (hAdv) detection with a specific real-time polymerase chain reaction (qPCR), which is considered the gold standard for hAdv detection, nasopharyngeal samples collected from 309 children (age range, four months to eight years) with respiratory tract infection were tested using the RVP Fast v2 assay (Luminex Molecular Diagnostics, Inc., Toronto, ON, Canada) and a specific TaqMan qPCR to identify hAdv DNA. The RVP Fast v2 assay detected 30/61 (49.2%) hAdv infections that had been identified by real-time qPCR, demonstrating a significantly lower detection rate (p < 0.001). The sensitivity of the RVP Fast v2 assay in comparison to qPCR was lower in younger children (42.9% vs. 57.7%; Cohen’s kappa coefficient, 0.53); in samples with co-infections (40.0% vs. 56.7%; Cohen’s kappa coefficient, 0.52); and in samples with hAdv type C (45.9% vs. 57.1%; Cohen’s kappa coefficient, 0.60). Samples with lower viral loads were associated with a significantly lower sensitivity of the RVP Fast v2 assay (35.1% vs. 68.2%, p = 0.01; Cohen’s kappa coefficients, 0.49). The RVP Fast v2 assay has important limitations for the detection of hAdv and cannot be used to evaluate whether hAdvs are the main etiologic agent responsible for an outbreak or when epidemiological studies are performed.     

Energy-saving self-configuring networked data centers

In this paper, we develop the optimal minimum-energy scheduler for the dynamic online joint allocation of the task sizes, computing rates, communication rates and communication powers in virtualized Networked Data Centers (NetDCs) that operates under hard per-job delay-constraints. The referred NetDC’s infrastructure is composed by multiple frequency-scalable Virtual Machines (VMs), that are interconnected by a bandwidth and power-limited switched Local Area Network (LAN). Due to the nonlinear power-vs.-communication rate relationship, the resulting Computing-Communication Optimization Problem (CCOP) is inherently nonconvex. In order to analytically compute the exact solution of the CCOP, we develop a solving approach that relies on the following two main steps: (i) we prove that the CCOP retains a loosely coupled structure, that allows us to perform the lossless decomposition of the CCOP into the cascade of two simpler sub-problems; and, (ii) we prove that the coupling between the aforementioned sub-problems is provided by a (scalar) constraint, that is linear in the offered workload. The resulting optimal scheduler is amenable of scalable and distributed online implementation and its analytical characterization is in closed-form. After numerically testing its actual performance under randomly time-varying synthetically generated and real-world measured workload traces, we compare the obtained performance with the corresponding ones of some state-of-the-art static and sequential schedulers.     

Participatory Irrigation Management and Transfer: Setting the Guiding Principles for a Sustaining Monitoring &amp; Evaluation System – a Focus on the Mediterranean

A major shortcoming of Participatory Irrigation Management and Irrigation Management Transfer (PIM/IMT) is the absence of Monitoring & Evaluation (M&E) systems that accompany these processes and become a major instrument to correct, improve and learn. Although at the global level some countries have exerted efforts to implement M&E systems, these still present limitations, as the review of the international experiences shows. The Mediterranean situation is even more dramatic since only Tunisia implements an M&E system, whereas Egypt has shyly moved in this direction with a limited practical application. This state of affairs led the authors to develop a set of guiding principles to develop an M&E system to be applied in countries where PIM/IMT is adopted. This paper revises the main international efforts devoted to assess the performance of PIM/IMT and the state of the art of these processes in Southern and Eastern Mediterranean Countries. Moreover, it sets the framework and analyses the main sustaining factors for the development of a new M&E system for these countries.     

Post-cracking behaviour of steel and macro-synthetic fibre-reinforced concretes

The present paper describes the results of an experimental investigation on the performances of concrete specimens reinforced with either steel or macro-synthetic fibres under three-point bending. Steel fibres are often used to improve the flexural toughness of concrete and are used in various structural applications while synthetic fibres are more often used to reduce crack opening due to shrinkage. Macro-synthetic fibres have been proposed more recently with the aim of creating an alternative to steel fibres in structural applications but their use is still limited.In the tests performed, specimens cast with the same concrete mix, but containing different dosages of either steel or macro-synthetic fibres, were used and compared. In general steel fibres were more efficient in increasing the toughness of concrete than macro-synthetic fibres, even though their results were significantly more scattered.Test results were used to calculate the parameters of stress-crack opening relations via inverse analysis using a cracked hinge model. This numerical model provided results, in terms of force-Crack Mouth Opening Displacement (CMOD) curves, in very good agreement with the experimental data.