Nicolas Magdelaine  Pablo S. Rivadeneira  Lucy Chaillous  Anne&#x;Laure Fournier&#x;Guilloux  Michel Krempf  Taghreed MohammadRidha  Mourad Ait&#x;Ahmed  Claude H. Moog 《IET systems biology》2020,14(1):16

Driving blood glycaemia from hyperglycaemia to euglycaemia as fast as possible while avoiding hypoglycaemia is a major problem for decades for type‐1 diabetes and is solved in this study. A control algorithm is designed that guaranties hypoglycaemia avoidance for the first time both from the theory of positive systems point of view and from the most pragmatic clinical practice. The solution consists of a state feedback control law that computes the required hyperglycaemia correction bolus in real‐time to safely steer glycaemia to the target. A rigorous proof is given that shows that the control‐law respects the positivity of the control and of the glucose concentration error: as a result, no hypoglycaemic episode occurs. The so‐called hypo‐free strategy control is tested with all the UVA/Padova T1DM simulator patients (i.e. ten adults, ten adolescents, and ten children) during a fasting‐night scenario and in a hybrid closed‐loop scenario including three meals. The theoretical results are assessed by the simulations on a large cohort of virtual patients and encourage clinical trials.Inspec keywords: biochemistry, medical control systems, blood, diseases, medical computing, closed loop systems, biomedical equipment, state feedback, patient treatment, patient monitoring, biomedical measurement, physiological models, sugarOther keywords: fasting‐night scenario, hybrid closed‐loop scenario, hypoglycaemia‐free artificial pancreas project, blood glycaemia, euglycaemia, type‐1 diabetes, control algorithm, guaranties hypoglycaemia avoidance, pragmatic clinical practice, state feedback control law, required hyperglycaemia correction bolus, rigorous proof, control‐law, glucose concentration error, hypo‐free strategy control  相似文献   

    

Jrg Krger  Nicolas Nel  Tim Oliver Wehling  Mads Brandbyge 《Small Methods》2020,4(5)

Inelastic electron tunneling spectroscopy with a scanning tunneling microscope is a powerful method to excite and detect vibrational quanta with atomic resolution. The focus of this review article is on the local spectroscopy of graphene phonons. The experimental observation of their spectroscopic signatures together with theoretical modeling highlight the importance of the graphene–surface as well as the graphene–tip hybridization, the electron–phonon coupling strength, phonon‐mediated tunneling, local doping profiles, and the phonon density of state for the measured signal. Meanwhile, a comprehensive understanding of the underlying mechanisms has been attained and justifies an overview on available findings.  相似文献   

    

Olivier Debauche  Sidi Ahmed Mahmoudi  Nicolas De Cock  Saïd Mahmoudi  Pierre Manneback  Frdric Lebeau 《Concurrency and Computation》2020,32(17)

Digital phenotyping is an emergent science mainly based on imagery techniques. The tremendous amount of data generated needs important cloud computing for their processing. The coupling of recent advance of distributed databases and cloud computing offers new possibilities of big data management and data sharing for the scientific research. In this paper, we present a solution combining a lambda architecture built around Apache Druid and a hosting platform leaning on Apache Mesos. Lambda architecture has already proved its performance and robustness. However, the capacity of ingesting and requesting of the database is essential and can constitute a bottleneck for the architecture, in particular, for in terms of availability and response time of data. We focused our experimentation on the response time of different databases to choose the most adapted for our phenotyping architecture. Apache Druid has shown its ability to respond to typical queries of phenotyping applications in times generally inferior to the second.  相似文献   

    

Alexandre Imperiale  Nicolas Leymarie  Edouard Demaldent 《International journal for numerical methods in engineering》2020,121(15):3300-3338

Composite laminate structures remain an important family of materials used in cutting-edge industrial areas. Building efficient numerical modeling tools for high-frequency wave propagation in order to represent ultrasonic testing experiments of these materials remains a major challenge. In particular, incorporating attenuation phenomena within anisotropic plies, and thin intermediate isotropic layers between the plies often represent significant obstacles for standard numerical approaches. In our work, we address both issues by proposing a systematic study of the fully discrete propagators associated to the Kelvin-Voigt, Maxwell, and Zener models, and by incorporating effective transmission conditions between plies using the mortar element method. We illustrate the soundness of our approach by proposing intermediate one-dimensional and two-dimensional numerical evidence, and we apply it to a more realistic configuration of a curved laminate composite structure in a three-dimensional setting.  相似文献   

    

Vítor M. Gaspar  Pedro Lavrador  João Borges  Mariana B. Oliveira  João F. Mano 《Advanced materials (Deerfield Beach, Fla.)》2020,32(6):1903975

Bottom-up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom-up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular-rich structures or multicomponent cell–biomaterial synergies are described. An up-to-date critical overview of long-term existing and rapidly emerging technologies for integrative bottom-up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.  相似文献   

    

Xiaoen Wang  Robert Kerr  Fangfang Chen  Nicolas Goujon  Jennifer M. Pringle  David Mecerreyes  Maria Forsyth  Patrick C. Howlett 《Advanced materials (Deerfield Beach, Fla.)》2020,32(18):1905219

With increasing demands for safe, high capacity energy storage to support personal electronics, newer devices such as unmanned aerial vehicles, as well as the commercialization of electric vehicles, current energy storage technologies are facing increased challenges. Although alternative batteries have been intensively investigated, lithium (Li) batteries are still recognized as the preferred energy storage solution for the consumer electronics markets and next generation automobiles. However, the commercialized Li batteries still have disadvantages, such as low capacities, potential safety issues, and unfavorable cycling life. Therefore, the design and development of electromaterials toward high-energy-density, long-life-span Li batteries with improved safety is a focus for researchers in the field of energy materials. Herein, recent advances in the development of novel organic electrolytes are summarized toward solid-state Li batteries with higher energy density and improved safety. On the basis of new insights into ionic conduction and design principles of organic-based solid-state electrolytes, specific strategies toward developing these electrolytes for Li metal anodes, high-energy-density cathode materials (e.g., high voltage materials), as well as the optimization of cathode formulations are outlined. Finally, prospects for next generation solid-state electrolytes are also proposed.  相似文献   

    

Ana Rita Sousa  Cláudia Martins-Cruz  Mariana B. Oliveira  João F. Mano 《Advanced materials (Deerfield Beach, Fla.)》2020,32(2):1906305

Cellular aggregates are used as relevant regenerative building blocks, tissue models, and cell delivery platforms. Biomaterial-free structures are often assembled either as 2D cell sheets or spherical microaggregates, both incompatible with free-form deposition, and dependent on challenging processes for macroscale 3D upscaling. The continuous and elongated nature of fiber-shaped materials enables their deposition in unrestricted multiple directions. Cellular fiber fabrication has often required exogenously provided support proteins and/or the use of biomaterial-based sacrificial templates. Here, the rapid (<24 h) assembly of fiberoids is reported: living centimeter-long scaffold-free fibers of cells produced in the absence of exogenous materials or supplements. Adipose-derived mesenchymal stem cell fiberoids can be easily modulated into complex multidimensional geometries and show tissue-invasive properties while keeping the secretion of trophic factors. Proangiogenic properties studied on a chick chorioallantoic membrane in an ovo model are observed for heterotypic fiberoids containing endothelial cells. These micro-to-macrotissues may find application as morphogenic therapeutic and tissue-mimetic building blocks, with the ability to integrate 3D and 4D full biological materials.  相似文献   

    

Giuseppe Schiavone  Florian Fallegger  Xiaoyang Kang  Beatrice Barra  Nicolas Vachicouras  Evgenia Roussinova  Ivan Furfaro  Sébastien Jiguet  Ismael Seáñez  Simon Borgognon  Andreas Rowald  Qin Li  Chuan Qin  Erwan Bézard  Jocelyne Bloch  Grégoire Courtine  Marco Capogrosso  Stéphanie P. Lacour 《Advanced materials (Deerfield Beach, Fla.)》2020,32(17):1906512

The convergence of materials science, electronics, and biology, namely bioelectronic interfaces, leads novel and precise communication with biological tissue, particularly with the nervous system. However, the translation of lab-based innovation toward clinical use calls for further advances in materials, manufacturing and characterization paradigms, and design rules. Herein, a translational framework engineered to accelerate the deployment of microfabricated interfaces for translational research is proposed and applied to the soft neurotechnology called electronic dura mater, e-dura. Anatomy, implant function, and surgical procedure guide the system design. A high-yield, silicone-on-silicon wafer process is developed to ensure reproducible characteristics of the electrodes. A biomimetic multimodal platform that replicates surgical insertion in an anatomy-based model applies physiological movement, emulates therapeutic use of the electrodes, and enables advanced validation and rapid optimization in vitro of the implants. Functionality of scaled e-dura is confirmed in nonhuman primates, where epidural neuromodulation of the spinal cord activates selective groups of muscles in the upper limbs with unmet precision. Performance stability is controlled over 6 weeks in vivo. The synergistic steps of design, fabrication, and biomimetic in vitro validation and in vivo evaluation in translational animal models are of general applicability and answer needs in multiple bioelectronic designs and medical technologies.  相似文献   

    

Alba Nicolas‐Boluda  Zhijie Yang  Illia Dobryden  Florent Carn  Naomi Winckelmans  Christine Pchoux  Pierre Bonville  Sara Bals  Per Martin Claesson  Florence Gazeau  Marie Paule Pileni 《Advanced functional materials》2020,30(40)

Control of interactions between nanomaterials and cells remains a biomedical challenge. A strategy is proposed to modulate the intralysosomal distribution of nanoparticles through the design of 3D suprastructures built by hydrophilic nanocrystals (NCs) coated with alkyl chains. The intracellular fate of two water‐dispersible architectures of self‐assembled hydrophobic magnetic NCs: hollow deformable shells (colloidosomes) or solid fcc particles (supraballs) is compared. These two self‐assemblies display increased cellular uptake by tumor cells compared to dispersions of the water‐soluble NC building blocks. Moreover, the self‐assembly structures increase the NCs density in lysosomes and close to the lysosome membrane. Importantly, the structural organization of NCs in colloidosomes and supraballs are maintained in lysosomes up to 8 days after internalization, whereas initially dispersed hydrophilic NCs are randomly aggregated. Supraballs and colloidosomes are differently sensed by cells due to their different architectures and mechanical properties. Flexible and soft colloidosomes deform and spread along the biological membranes. In contrast, the more rigid supraballs remain spherical. By subjecting the internalized suprastructures to a magnetic field, they both align and form long chains. Overall, it is highlighted that the mechanical and topological properties of the self‐assemblies direct their intracellular fate allowing the control intralysosomal density, ordering, and localization of NCs.  相似文献   

    

Yaping Chen  Ji Wang  Xiangling Li  Ning Hu  Nicolas H. Voelcker  Xi Xie  Roey Elnathan 《Advanced materials (Deerfield Beach, Fla.)》2020,32(40):2001668

Engineered nano–bio cellular interfaces driven by 1D vertical nanostructures (1D-VNS) are set to prompt radical progress in modulating cellular processes at the nanoscale. Here, tuneable cell–VNS interfacial interactions are probed and assessed, highlighting the use of 1D-VNS in immunomodulation, and intracellular delivery into immune cells—both crucial in fundamental and translational biomedical research. With programmable topography and adaptable surface functionalization, 1D-VNS provide unique biophysical and biochemical cues to orchestrate innate and adaptive immunity, both ex vivo and in vivo. The intimate nanoscale cell–VNS interface leads to membrane penetration and cellular deformation, facilitating efficient intracellular delivery of diverse bioactive cargoes into hard-to-transfect immune cells. The unsettled interfacial mechanisms reported to be involved in VNS-mediated intracellular delivery are discussed. By identifying up-to-date progress and fundamental challenges of current 1D-VNS technology in immune-cell manipulation, it is hoped that this report gives timely insights for further advances in developing 1D-VNS as a safe, universal, and highly scalable platform for cell engineering and enrichment in advanced cancer immunotherapy such as chimeric antigen receptor-T therapy.  相似文献