全文获取类型
收费全文 | 2600篇 |
免费 | 164篇 |
国内免费 | 5篇 |
专业分类
电工技术 | 37篇 |
综合类 | 1篇 |
化学工业 | 675篇 |
金属工艺 | 51篇 |
机械仪表 | 46篇 |
建筑科学 | 68篇 |
矿业工程 | 4篇 |
能源动力 | 90篇 |
轻工业 | 244篇 |
水利工程 | 21篇 |
石油天然气 | 2篇 |
无线电 | 232篇 |
一般工业技术 | 540篇 |
冶金工业 | 150篇 |
原子能技术 | 18篇 |
自动化技术 | 590篇 |
出版年
2024年 | 5篇 |
2023年 | 39篇 |
2022年 | 83篇 |
2021年 | 113篇 |
2020年 | 85篇 |
2019年 | 76篇 |
2018年 | 80篇 |
2017年 | 84篇 |
2016年 | 108篇 |
2015年 | 94篇 |
2014年 | 123篇 |
2013年 | 170篇 |
2012年 | 204篇 |
2011年 | 229篇 |
2010年 | 171篇 |
2009年 | 150篇 |
2008年 | 153篇 |
2007年 | 127篇 |
2006年 | 127篇 |
2005年 | 80篇 |
2004年 | 72篇 |
2003年 | 60篇 |
2002年 | 42篇 |
2001年 | 25篇 |
2000年 | 21篇 |
1999年 | 23篇 |
1998年 | 45篇 |
1997年 | 32篇 |
1996年 | 19篇 |
1995年 | 14篇 |
1994年 | 15篇 |
1993年 | 19篇 |
1992年 | 5篇 |
1991年 | 15篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1981年 | 5篇 |
1979年 | 6篇 |
1975年 | 3篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1967年 | 1篇 |
1931年 | 1篇 |
排序方式: 共有2769条查询结果,搜索用时 19 毫秒
71.
Guusje van Schaick Nadi el Hajjouti Simone Nicolardi Joost den Hartog Romana Jansen Rob van der Hoeven Wim Bijleveld Nicolas Abello Manfred Wuhrer Maurien M. A. Olsthoorn Elena Domínguez-Vega 《International journal of molecular sciences》2022,23(3)
Xylanases are of great value in various industries, including paper, food, and biorefinery. Due to their biotechnological production, these enzymes can contain a variety of post-translational modifications, which may have a profound effect on protein function. Understanding the structure–function relationship can guide the development of products with optimal performance. We have developed a workflow for the structural and functional characterization of an endo-1,4-β-xylanase (ENDO-I) produced by Aspergillus niger with and without applying thermal stress. This workflow relies on orthogonal native separation techniques to resolve proteoforms. Mass spectrometry and activity assays of separated proteoforms permitted the establishment of structure–function relationships. The separation conditions were focus on balancing efficient separation and protein functionality. We employed size exclusion chromatography (SEC) to separate ENDO-I from other co-expressed proteins. Charge variants were investigated with ion exchange chromatography (IEX) and revealed the presence of low abundant glycated variants in the temperature-stressed material. To obtain better insights into the effect on glycation on function, we enriched for these species using boronate affinity chromatography (BAC). The activity measurements showed lower activity of glycated species compared to the non-modified enzyme. Altogether, this workflow allowed in-depth structural and functional characterization of ENDO-I proteoforms. 相似文献
72.
Aivar Sootla Nicolas Delalez Emmanouil Alexis Arthur Norman Harrison Steel George H. Wadhams Antonis Papachristodoulou 《Journal of the Royal Society Interface》2022,19(189)
We introduce a new design framework for implementing negative feedback regulation in synthetic biology, which we term ‘dichotomous feedback’. Our approach is different from current methods, in that it sequesters existing fluxes in the process to be controlled, and in this way takes advantage of the process’s architecture to design the control law. This signal sequestration mechanism appears in many natural biological systems and can potentially be easier to realize than ‘molecular sequestration’ and other comparison motifs that are nowadays common in biomolecular feedback control design. The loop is closed by linking the strength of signal sequestration to the process output. Our feedback regulation mechanism is motivated by two-component signalling systems, where a second response regulator could be competing with the natural response regulator thus sequestering kinase activity. Here, dichotomous feedback is established by increasing the concentration of the second response regulator as the level of the output of the natural process increases. Extensive analysis demonstrates how this type of feedback shapes the signal response, attenuates intrinsic noise while increasing robustness and reducing crosstalk. 相似文献
73.
Stefano Panebianco Klara Berg Jean-Christophe David Mohamed Eid Uwe Filges Friedrich Gröschel Arnaud Guertin Alexander Yu Konobeyev Christian Latgé Sébastien Lemaire Sylvie Leray Alain Letourneau Markus Lüthy Franco Michel-Sendis Selene Scazzi Gediminas Stankunas Nicolas Thiollière Leonhard Tobler Luca Zanini 《Annals of Nuclear Energy》2009
The MEGAPIE project aimed to design, build and operate a liquid metal spallation neutron target of about 1 MW beam power in the SINQ facility at the Paul Scherrer Institut (Villigen, Switzerland). This project is an important step in the roadmap towards the demonstration of the accelerator driven system (ADS) concept and high power liquid metal targets in general. Following the design phase, an experimental program was defined to provide a complete characterization of the facility by performing a “mapping” of the neutron flux at different points, from the center of the target to the beam lines. The neutronic performance of the target was studied using different experimental techniques with the goals of validating the Monte Carlo codes used in the design of the target; additionally, the performance was compared with the solid lead targets used before and after the MEGAPIE experiment. 相似文献
74.
Andreas Mettler Nicolas Wyrsch Michael Goetz Arvind Shah 《Solar Energy Materials & Solar Cells》1994,34(1-4)
We present systematic measurements of CPM on two independent series of slightly phosphorous and boron doped films. For “n-type” samples of both series, the CPM deep defect absorption is proportional to the square root of the gas dopant ratio. For these samples we discuss the influence of Fermi level on the CPM spectra. For slightly “p-type” samples, CPM deep defect absorption as evaluated by CPM becomes higher than the corresponding PDS-values. This fundamental problem can be traced back to the violation of two basic conditions necessary for a correct evaluation of the absorption from CPM measurements: (1) the power law exponent γ (Rose factor) of the photoconductivity must be spectrally independent, and (2) the generation rate G, which corresponds to the CPM photocurrent, also has to be spectrally independent. Further, we compare the annealed and the “saturated” light soaked states of selected slightly doped samples and an undoped sample: the variations in the CPM deep defect absorption and in photoconductivity due to light-soaking are discussed. 相似文献
75.
Jean Claude Assaf Andr El Khoury Ali Chokr Nicolas Louka Ali Atoui 《International Journal of Dairy Technology》2019,72(2):248-256
This study aimed to develop a new method for detoxification of milk from aflatoxin M1 (AFM1) by using Lactobacillus rhamnosus GG biofilm. After inoculation of milk contaminated with AFM1 into L. rhamnosus GG biofilm, the unbound AFM1 was extracted and quantified by HPLC. The stability of the formed AFM1/biofilm complex using different AFM1 contamination levels of milk was also studied. We found that the percentages of bound AFM1 by L. rhamnosus GG biofilm reached up to 60.74%. While no significant difference in milk proteins content was observed after AFM1 binding, some changes in total dry matter and fat content were noticed. 相似文献
76.
77.
78.
79.
Mariana Tasso Thomas Pons Nicolas Lequeux Julie Nguyen Zsolt Lenkei Diana Zala 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(47)
Single‐particle tracking with quantum dots (QDs) constitutes a powerful tool to track the nanoscopic dynamics of individual cell membrane components unveiling their membrane diffusion characteristics. Here, the nano‐resolved population dynamics of QDs is exploited to reconstruct the topography and structural changes of the cell membrane surface with high temporal and spatial resolution. For this proof‐of‐concept study, bright, small, and stable biofunctional QD nanoconstructs are utilized recognizing the endogenous neuronal cannabinoid receptor 1, a highly expressed and fast‐diffusing membrane protein, together with a commercial point‐localization microscope. Rapid QD diffusion on the axonal plasma membrane of cultured hippocampal neurons allows precise reconstruction of the membrane surface in less than 1 min with a spatial resolution of tens of nanometers. Access of the QD nanoconstructs to the synaptic cleft enables rapid 3D topological reconstruction of the entire presynaptic component. Successful reconstruction of membrane nano‐topology and deformation at the second time‐scale is also demonstrated for HEK293 cell filopodia and axons. Named “nanoPaint,” this super‐resolution imaging technique amenable to any endogenous transmembrane target represents a versatile platform to rapidly and accurately reconstruct the cell membrane nano‐topography, thereby enabling the study of the rapid dynamic phenomena involved in neuronal membrane plasticity. 相似文献
80.
M Mirshahi S Mirshahi N Golestaneh C Nicolas Z Mishal MK Agarwal 《Canadian Metallurgical Quarterly》1998,24(3-4):455-459
A Cell extract from the HEL (human erythroblastic leukemia) cell line was positive for both the epithelial sodium channel (ENaC) and the mineralocorticoid receptor (MCR) as glycosylated 82-84 kDa bands, and a single 102 kDa band, respectively, in Western blots using polyclonal antibodies raised against these proteins. The immunofluorescent labeling of the MCR in all cell lines showed a nucleocytoplasmic localization of the receptor whereas the ENaC was exclusively membrane-bound. These results were confirmed by confocal microscopy. The expression of the MCR in HEL cells was evident as a predicted band of 843 bp (234 amino acids) after total RNA from HEL cells had been reverse transcribed and then amplified by PCR; the ENaC was similarly evident as a predicted band of 520 bp. In both cases, near 100% identity was observed between the deduced amino acid sequences of the PCR products and those from known human sources. The multiplication of HEL cells was influenced by antagonists (RU 26752, ZK 91587) targeted for specificity to the MCR and this was reversed by the natural hormone aldosterone. These steroids also provoked chromatin condensation in the HEL population. 相似文献