The p35/Cdk5 kinase is a neuron-specific Rac effector that inhibits Pak1 activity

Cyclin-dependent kinase 5 (Cdk5) and its neuron-specific regulator p35 are essential for neuronal migration and for the laminar configuration of the cerebral cortex. In addition, p35/Cdk5 kinase concentrates at the leading edges of axonal growth cones and regulates neurite outgrowth in cortical neurons in culture. The Rho family of small GTPases is implicated in a range of cellular functions, including cell migration and neurite outgrowth. Here we show that the p35/Cdk5 kinase co-localizes with Rac in neuronal growth cones. Furthermore, p35 associates directly with Rac in a GTP-dependent manner. Another Rac effector, Pak1 kinase, is also present in the Rac-p35/Cdk5 complexes and co-localizes with p35/Cdk5 and Rac at neuronal peripheries. The active p35/Cdk5 kinase causes Pak1 hyperphosphorylation in a Rac-dependent manner, which results in down-regulation of Pak1 kinase activity. Because the Rho family of GTPases and the Pak kinases are implicated in actin polymerization, the modification of Pak1, imposed by the p35/Cdk5 kinase, is likely to have an impact on the dynamics of the reorganization of the actin cytoskeleton in neurons, thus promoting neuronal migration and neurite outgrowth.     

Performance of PSK signal transmitted over satellite channel

The authors determine the bit error rate of a digital satellite system with phase modulation when at the input of the satellite only Gaussian noise is added, while at the input of the earth station they assume both Gaussian noise and an interference approximated by the sum of n sinusoidal signals     

CDMA bus-based on-chip interconnect infrastructure

As technology scales toward deep submicron, the integration of complete system-on-chip (SoC) designs consisting of large number of Intellectual Property (IP) blocks (cores) on the same silicon die is becoming technically feasible. Until recently, the design-space exploration for SoCs has been mainly focused on the computational aspects of the problem. However, as the number of IP blocks on a single chip and their performance continue to increase, a shift from computation-based to communication-based designs becomes mandatory. As a result, the communication architecture plays a major role in the area, performance and energy consumption of the overall systems [Pasricha S, Dutt N. On-chip communication architectures: system on chip interconnect. Amsterdam: Elsevier Inc.; 2008, Kim J, Verbauwhede I, Chang MCF. Design of an interconnect architecture and signaling technology for parallelism in communication. IEEE Trans VLSI Syst 2007;15(8):881-94].This article presents a structure of a wrapper as a component of Code Division Multiple Access, CDMA, based shared bus architecture in a SoC. Two types of wrappers can be identified, master and slave. A master wrapper is located between the arbiter and CDMA coded physical interconnect, while a slave connects the CDMA coded bus with memory/peripheral module. In the proposal, only bus lines that carry address and data signals are CDMA coded. We implemented a pair of master-slave wrapper described in VHDL and confirmed its functionality using testbenches. Also we synthesized wrappers using a Xilinx Spartan and Virtex devices to determine resource requirements in respect to a number of equivalent gates, communication bandwidth, latency and power consumption. Specifically we involved a Design_Quality, DQ, metric for wrapper performance evaluation. A pair of master-slave wrapper seems to occupy appropriate space, in average 2000 equivalent gates, considering CPU cost of about 30,000 gates, what is less than 8% of hardware overhead per CPU. We also present experimental results which show that benefits of involving CDMA coding relates both to decreasing a number of bus lines and accomplishing simultaneous multiple master-slave connections at relatively low-power consumption and high communication bandwidth. Convenient range indices R_W and R_R to determine data transfer rate for Write and Read operations in multiprocessor bus systems that use TDMA and CDMA data transfer techniques. The obtained results show that increased data transfer latencies involved by CDMA data transfer are compensated by simultaneous master-slave transfers.     

Frequency and Timing Offset Estimation Using Single Reference Symbol

In this paper we will investigate the performance of an estimator employing single OFDM reference symbol for timing and frequency offset estimation. We will show how both coarse and fine frequency offset estimation can be performed with this single OFDM reference symbol. The performance of this estimator will be compared with the ML estimator employing OFDM reference symbols of different length for coarse and fine frequency offset estimation. It will be shown that, unlike ML estimator, the estimation range of the analyzed estimator is not constrained.     

Investigation of ZnFe2O4 spinel ferrite nanocrystalline screen-printed thick films for application in humidity sensing

Zinc ferrite nanocrystalline powder was obtained by solid state synthesis of starting zinc oxide and hematite nanopowders. Field emission scanning electron microscopy and transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and Raman spectroscopy confirmed the formation of nanocrystalline zinc-ferrite powder with a mixed spinel structure with small amounts of remaining zinc oxide and hematite as impurities. Thick film paste was formed and screen printed on test interdigitated PdAg electrodes on alumina substrate. Formation of a porous nanocrystalline structure was confirmed by scanning electron microscopy and Hg porosimetry. Humidity sensing properties of zinc ferrite thick films were investigated by monitoring the change in impedance in the relative humidity interval 30%-90% in the frequency range 42 Hz-1 MHz at room temperature (25°C) and 50°C. At 42 Hz at both analyzed temperatures the impedance reduced ~46 times in the humidity range 30%-90%. The dominant influence of grain boundaries was confirmed by analysis of complex impedance with an equivalent circuit.     

Characterization of lactic acid bacteria isolated from Bukuljac, a homemade goat's milk cheese

The Bukuljac cheese is traditionally homemade cheese, produced from heat-treated goat's milk without the addition of any bacterial starter culture. The presence of lactic acid bacteria (LAB) in Bukuljac cheese has been analyzed by using a polyphasic approach including microbiological and molecular methods such as rep-PCR with (GTG)5 primer. Lactobacillus paracasei subsp. paracasei represents a dominant strain in the microflora of analyzed cheese. Out of 55 Gram-positive and catalase-negative isolates, 48 belonged to L. paracasei subsp. paracasei species. Besides lactobacilli, five Lactococcus lactis subsp. lactis and two Enterococcus faecalis were found. Results of PCR-denaturing gradient gel electrophoresis (DGGE) of DNA extracted directly from the fresh cheese revealed the presence of Leuconostoc mesenteroides. Only lactobacilli showed a high proteolytic activity and hydrolyzed alpha(s1)- and beta-caseins. They are also producers of diacetyl. In addition, 34 out of 55 isolates, all determined as lactobacilli, showed the ability of auto-aggregation. Among 55 isolates, 50 also exhibited antimicrobial activity.