Quantitative evaluation of bone resorption activity of osteoclast‐like cells by measuring calcium phosphate resorbing area using incubator‐facilitated and video‐enhanced microscopy

Quantitative evaluation of the ability of bone resorption activity in live osteoclast‐like cells (OCLs) has not yet been reported on. In this study, we observed the sequential morphological change of OCLs and measured the resorbing calcium phosphate (CP) area made by OCLs alone and with the addition of elcatonin utilizing incubator facilitated video‐enhanced microscopy. OCLs, which were obtained from a coculture of ddy‐mouse osteoblastic cells and bone marrow cells, were cultured on CP‐coated quartz cover slips. The CP‐free area increased constantly in the OCLs alone, whereas it did not increase after the addition of elcatonin. This study showed that analysis of the resorbed areas under the OCL body using this method enables the sequential quantitative evaluation of the bone resorption activity and the effect of several therapeutic agents on bone resorption in vitro. Microsc. Res. Tech, 2009. © 2008 Wiley‐Liss, Inc.     

Computed tomography reconstruction from two transmission measurements for iodine-marked cancer detection

The authors invented the transXend detector, which measures X-rays as electric currents, and then gives the energy distribution of the X-rays after an unfolding process. In a previous paper, it was shown that the material thickness distributions can be estimated with the transXend detector by using reference points plotted from the electric current ratios, such as the I ₂/I ₁ ? I ₃/I ₁ graph, where I_i denotes the electric current measured by the i-th segment of the transXend detector. In this paper, the tomographic images of iodine, aluminum, and the acrylic those surround the other two materials are reconstructed from their material thickness distributions, which are estimated from two X-ray incidence directions. The X-ray event ratios are also used to estimate the material thickness distributions.     

Remarkable effect of ordered mesoporous carbon support in tantalum oxide-catalyzed selective epoxidation of cyclooctene

The olefin epoxidation is one of the most important reactions in chemical industry. Metal oxide supports often cause drawbacks in catalytic activity and selectivity, which has been overcome by introducing hydrophobic organic groups onto the oxide supports. The present study utilizes ordered mesoporous carbon (CMK-3 and CMK-1) as structurally defined hydrophobic catalyst support. Well-dispersed tantalum oxides supported on the ordered mesoporous carbon were prepared. Their application in catalytic epoxidation of cyclooctene demonstrates that the tantalum oxide catalysts on the ordered mesoporous carbon supports show higher performances than those of the catalysts supported on activated carbon and ordered mesoporous silica SBA-15.     

Viewpoints to characterize precision evaluation methods in binary measurements

Precision evaluation in quantitative measurements is a thoroughly discussed topic and the established methods are in use. Many methods are proposed for qualitative data including binary data, but their effectiveness and statistical properties are not so clear.     

CRACK INITIATION AND PROPAGATION BEHAVIOUR OF A HEAT-TREATED CARBON STEEL IN CORROSION FATIGUE

Abstract— When estimating fatigue damage quantitatively it is important to clarify its physical basis. In this study, rotating bending fatigue tests of a heat-treated 0.45% carbon steel were carried out in 3% NaCl solution, in order to clarify the physical basis of corrosion fatigue damage from successive observations of plastic replicas. The results show that corrosion pits are generated during the early stages of cycling, then most of them grow with further cycling until a crack is initiated from each corrosion pit. The initiation of corrosion pits from slip bands is observed only in the case when the stress range is relatively large, and in the range of stress for which slip bands are produced in air. After initiation of a crack, the crack propagates by frequent interactions and coalescence with other cracks. The growth rate of an especially small crack in NaCl solution is larger than that in air. However, the growth rate of a comparatively large crack is smaller in NaCl solution than in air.     

Hydrogen absorption properties of Li–Mg–N–H system

Isothermal hydrogen absorption properties of the ball milled mixture of 3Mg(NH₂)₂ and 8LiH after dehydrogenation at 200 °C under high vacuum were investigated at two different temperatures of 150 and 200 °C. The pressure–composition isotherm (PCT) curve at 200 °C revealed a two-plateaus-like behavior, while the PCT curve at 150 °C showed a single-plateau-like behavior. The hydrogenated phases were composed of LiH and Mg(NH₂)₂ under 9 MPa at 200 °C, while those were observed as mixed phases of LiH and LiNH₂ at 150 °C without any trace of Mg(NH₂)₂ in XRD measurements. These results indicate that there are two-step hydrogenation processes corresponding to high and low pressures at 200 °C, but the kinetics at 150 °C is too slow to proceed with the second hydrogenating step at high pressure region.     

Application of genetic algorithm to speed servo system and positioning system

This paper considers a genetic algorithm application to feedforward controllers of speed servo and positioning systems. The system consists of a conventional feedback controller and a feedforward controller. The parameters of the feedforward controller are regarded as the chromosome on a gene. The genetic algorithm is used to tune the parameters of the feedforward controller, the objective of which is to make the feedforward controller a dynamic inverse of the plant. The tracking error between the desired output and the actual output at every sampling period is used to calculate the fitness of one gene. The range of mutation is dependent on the magnitude of the tracking error, which ensures parameter convergence. The effect of fitness function and the width of mutation on tracking performances is investigated. The experimental results demonstrate the effectiveness of the proposed genetic algorithm in improving tracking performances.     

Stress concentration of a strip with double edge notches under tension or in-plane bending

The stress concentration analysis of 60° V-shaped or partially-circular double edge notches in an infinite strip under tension or in-plane bending is discussed. The stress field induced by a point force in an infinite plate is used to solve these problems. The present results for semicircular notch are in close agreement with other reports. The results calculated on the 60° V-shaped notches show that the Neuber formula gives an underestimated stress concentration factor of about 11% for tension case and in about 9% for bending case. These errors exist for a wide range of notch depth. However, in the case of blunt notches, the Neuber solution of deep hyperbolic notches still gives a sufficient accuracy in engineering use. In addition, the stress concentration factors of 60° V-shaped notches are also represented by diagrams for wide use.     

A Study on Error Recovery Search Strategies of Electronic Connector Mating for Robotic Fault-Tolerant Assembly

In this paper, a CAD-based trajectory planning scheme for parallel machining robots is introduced using the parametric Non-uniform rational basis spline (NURBS) curves. First, a trajectory is designed via a NURBS curve then, a motion scheduling architecture consisting of time-dependent and constant feedrate profiles is advised to generate the position commands on the represented NURBS curve as the tool path. Using the generated commands, the inverse kinematics is elaborated to obtain the joints motions of the parallel machining robot. This paper investigates the NURBS trajectory generation for a parallel robot with 4(UPS)-PU mechanism as the case study. In order to evaluate the effectiveness of the proposed method, the inverse kinematic results for the parallel machining robot of 4(UPS)-PU is compared with the simulation results obtained from the CATIA software. The results confirmed that the proposed trajectory planning scheme along with the advised motion planning architecture is not only feasible for the parallel machining robots but also yields a smooth trajectory with a satisfactory performance for all the joints.