The effect of the N-linked glycans on structural features and physicochemical functions of soybean β-conglycinin homotrimers

β-Conglycinin is a trimeric protein consisting of three subunits, α,α′,and β, which are N-glycosylated. The α and α′ subunits contain extension regions in addition to core regions common to all subunits. We purified homogeneous trimers consisting of only α, α′, or β from mutant soybean cultivars containing β-conglycinin lacking one or two subunits: α homotrimers from an α′-lacking mutant, α′ homotrimers from an α-lacking mutant, and β homotrimers from an α-and α′-lacking mutant. Structural features and physicochemical functions of the three homotrimers were examined and compared with those of recombinant homotrimers having no N-linked glycans. The native homotrimers have secondary structures very similar to those of the recombinant ones. In analogy with the recombinant homotrimers, the native ones exhibit different thermal stabilities from one another (β>α′>α), and the native α and α′ homotrimers exhibit better solubility, emulsifying ability, and heat-induced association than the native β homotrimer. Further, the N-linked glycans contribute to solubilities of the three subunits at low ionic strength (μ=0.08) and to the emulsifying ability of the native β homotrimer. N-Linked glycans also prevent heat-induced associations of the native α and α′ homotrimers but do not contribute to the secondary structure and the thermal stability of β-conglycinin.     

Unique molecular aggregation of novel naphthalocyanine (near IR absorbing dye)

A series of organic-soluble naphthalocyanine derivatives (Y_mMNcX₄) have been synthesized. Their spectroscopic properties in organic solutions and in thin films were studied. MNcX₄ such as MNc(t-Bu)₄ (5a-5c) and MNc(On-Bu)₄ (5j) formed H-aggregates even in dilute solutions. MNc(CO₂R')₄ (5d-5i) had much stronger H-aggregation properties compared with those ofMNc(t-Bu)₄ and MNc(On-Bu)₄. Especially, MNc(CO₂R')₇ complexes (M=Cu, Pd and Ni) existed exclusively without monomers in the aggregated state even in highly dilute solutions. MNcX₄, which has strong H-aggregation properties, showed merely H- aggregate absorption maxima in thin films. In contrast, Y₂MNcX₄ (4), which has sterically hindered Y groups, showed monomerically pure characteristics in solution. However, thin films of Y₂MNcX₄ have a J-type molecular arrangement, exhibiting a red shift of Q-band absorption. The monomeric properties of Y₂MNcX₄ in solutions and J-type molecular arrangement in thin films arise from steric hindrance of two Y groups, such as R₃SiO-in Y₂MNcX₄, which prevents strong H-aggregation of naphthalocyanine.     

Preparation of siloxane-like films by glow discharge polymerization

Glow discharge polymerizations of tetramethoxysilane (TMOS), tetramethylsilane/oxygen mixture (½ molar ratio) (TMS/O₂), hexamethyldisiloxane (HMDSO), and tetramethyldisiloxane (TMDSO) were carried out for the preparation of thin, polymeric films with siloxane structures. The chemical composition of the formed polymers was examined by elemental analysis, infrared spectroscopy, and electron spectroscopy for chemical analysis (ESCA) in connection with polymerization conditions, especially, a level of the radiofrequency (rf) input power per mass of the monomers (W/FM value). The polymers prepared from HMDSO at fairly low W/FM values resembled in chemical composition the conventionally polymerized polydimethylsiloxane. The surface properties of the formed polymers also were evaluated by the measurement of surface energy.     

Effects of amino acid replacements around the reactive site of chicken ovomucoid domain 3 on the inhibitory activity toward chymotrypsin and trypsin

We have previously shown that replacing the P1-site residue(Ala) of chicken ovomucoid domain 3 (OMCHI3) with a Met or Lysresults in the acquisition of inhibitory activity toward chymotrypsinor trypsin, respectively. However, the inhibitory activitiesthus induced are not strong. In the present study, we introducedadditional amino acid replacements around the reactive siteto try to make the P1-site mutants more effective inhibitorsof chymotrypsin or trypsin. The amino acid replacement AspTyrat the P2' site of OMCHI3(P1Met) resulted in conversion to a35000-fold more effective inhibitor of chymotrypsin with aninhibitor constant (K_i) of 1.17x10^–11 M. The K_i valueof OMCHI3(P1Met, P2'Ala) indicated that the effect on the interactionwith chymotrypsin of removing a negative charge from the P2'site was greater than that of introducing an aromatic ring.Similarly, enhanced inhibition of trypsin was observed whenthe AspTyr replacement was introduced into the P2' site of OMCHI3(P1Lys).Two additional replacements, AspAla at the P4 site and ArgAlaat the P3' site, made the mutant a more effective inhibitorof trypsin with a K_i value of 1.44x10^–9 M. By contrast,ArgAla replacement at the P3' site of OMCHI3(P1Met, P2'Tyr)resulted in a greatly reduced inhibition of chymotrypsin, andAspAla replacement at the P4 site produced only a small changewhen compared with a natural variant of OMCHI3. These resultsclearly indicate that not only the P1-site residue but alsothe characteristics, particularly the electrostatic properties,of the amino acid residues around the reactive site of the proteaseinhibitor determine the strength of its interactions with proteases.Furthermore, amino acids with different characteristics arerequired around the reactive site for strong inhibition of chymotrypsinand trypsin.     

Uniformly Porous Composites with 3-D Network Structure (UPC-3D) for High-Temperature Filter Applications

Uniformly porous composites with 3-D network structure (UPC-3D) have been recently developed via a pyrolytic reactive sintering process, which takes advantage of the evolved CO₂ gas from a decomposing carbonate source (e.g., dolomite, CaMg(CO₃)₂) and does not require any additional pore-forming agent nor long-time burning-out process. Through liquid formation via LiF doping, strong necks are formed between constituent particles before completion of the pyrolysis of carbonate, resulting in the formation of a strong 3-D network structure. The pore size distribution is very narrow (with typical pore size: ∼1 μm), and the porosity was controllable (∼30–60%) by changing the sintering temperature. This article presents the development details of UPC-3D, and reports the recent findings in CaZrO₃/MgAl₂O₄ system, which will be one of the more promising systems for practical applications.     

Head to head polymers

Blends of poly(-caprolactone) with either head-to-head or head-to-tail poly (vinyl chloride) were prepared by solvent casting in the form of films and their glass transition temperatures were determined by DSC analysis. The DSC scans of the blends of both poly(vinyl chloride) isomers were very similar. For blends containing 25% and 50% of poly(-caprolactone), a single transition step, a T_g, was observed, whereas for a blend containing 75% of poly (-caprolactone) three transition steps were observed, one glass transition and also a crystallization and a melting transition.Part 19: H. Kawaguchi, J. Muggee, Y. Sumida and O. Vogl, Polymer (London), in press     

Lab-scale feasibility study on new elastomer comprising chloroprene and acrylonitrile for oil and gas applications

The limited options of suitable elastomers with adequate cost-performance balance drive the necessity to introduce new materials in the oil & gas (O&G) application space. The relevance of a recently developed copolymer of chloroprene and acrylonitrile (referred to as acrylonitrile–chloroprene rubber or NCR) to O&G applications is described in this technical contribution. The new elastomer demonstrates adequate physical properties and reasonably good high and low-temperature capabilities. It offers good resistance to several aqueous and non-aqueous fluids with low volume swelling and retains its physical properties to reasonable extents while exposed to hot test oils. Acrylonitrile–chloroprene rubber has been found to sustain “sour gas” exposure. Good abrasion resistance, high tear strength, and remarkably high flex-fatigue resistance coupled with low heat build-up reflect its durability under dynamic conditions. In addition, acrylonitrile–chloroprene rubber can very well withstand the rapid gas decompression test at 25°C. This unique combination of attributes may allow acrylonitrile–chloroprene rubber to be considered as a candidate material for high-performance O&G applications.     

IMPROVEMENT OF TYPE IV CRACKING RESISTANCE OF 9Cr HEAT RESISTING STEEL WELDMENT BY BORON ADDITION

Creep lives of high Cr ferritic heat resisting steel weldments decrease due to Type Ⅳ fracture, which occurs as a result of formation and growth of creep voids and cracks on grain boundaries in fine-grained heat affected zone (HAZ). Because boron is considered to suppress the coarsening of grain boundary precipitates and growth of creep voids, we have investigated the effect of boron addition on the creep properties of 9Cr steel weldments. Four kinds of 9Cr3WSCoVNb steels with boron content varying from 4.7×10-5 to 1.8×10-4 and with nitrogen as low as 2.0×10-5 were prepared. The steel plates were welded by gas tungsten arc welding and crept at 923K. It was found that the microstructures of HAZ were quite different from those of conventional high Cr steels such as P91 and P92, namely the fine-grained HAZ did not exist in the present steel weldments. Boron addition also has the effect to suppress coarsening of grain boundary carbides in HAZ during creep. As a result of these phenomena, the welded join     

Thermal management of edge‐lit wide color gamut backlight for LCD using red laser diodes and cyan LEDs

The color gamut is one of the critical parameters that dictate the image quality of displays. The liquid crystal displays using white color light‐emitting diodes (LEDs) as the backlight, though having been widely employed recently, are not very satisfactory in terms of their color gamut because of the broad spectrum inherent to white LEDs. This prompted the authors to develop improved liquid crystal displays using an edge‐lit wide color gamut backlight that used red laser diodes and cyan LEDs. Generating laser beams with high color purity, the laser diodes are light sources with a significant effect on expanding the color gamut. However, laser diodes, red ones in particular, have unfavorable thermal characteristics. To cope with this shortcoming, the authors clearly defined the restrictive criteria for laying out two kinds of light source on the edge‐lit backlight and made a prototype 55‐type laser backlight for performance evaluation.     

A Recommendation System Keeping Both Precision and Recall by Extraction of Uninteresting Information

A recommendation system which recommends interesting information to the target user must guarantee high precision and recall. However, there is trade-off between precision and recall. In this paper, we propose a web page recommendation method balancing both of them by take advantage of uninteresting information. The proposed method extracts the interest and uninterest indicators from not only historical interesting web pages but also uninteresting ones in a target genre. The historical interesting and uninteresting information is derived based on the browsing time and bookmarking. The proposed method can keep precision and recall by excluding the uninteresting information from the recommended ones based on the interest and uninterest indicators. The experimental result proved that the proposed method can improve the precision and recall than an existing method.