全文获取类型
收费全文 | 1339篇 |
免费 | 31篇 |
专业分类
电工技术 | 2篇 |
化学工业 | 115篇 |
金属工艺 | 9篇 |
机械仪表 | 16篇 |
建筑科学 | 17篇 |
能源动力 | 18篇 |
轻工业 | 91篇 |
水利工程 | 10篇 |
石油天然气 | 1篇 |
无线电 | 26篇 |
一般工业技术 | 51篇 |
冶金工业 | 946篇 |
自动化技术 | 68篇 |
出版年
2024年 | 3篇 |
2023年 | 5篇 |
2022年 | 18篇 |
2021年 | 14篇 |
2020年 | 9篇 |
2019年 | 17篇 |
2018年 | 20篇 |
2017年 | 13篇 |
2016年 | 22篇 |
2015年 | 14篇 |
2014年 | 23篇 |
2013年 | 28篇 |
2012年 | 23篇 |
2011年 | 40篇 |
2010年 | 25篇 |
2009年 | 20篇 |
2008年 | 18篇 |
2007年 | 18篇 |
2006年 | 18篇 |
2005年 | 18篇 |
2004年 | 13篇 |
2003年 | 15篇 |
2002年 | 8篇 |
2001年 | 8篇 |
2000年 | 6篇 |
1999年 | 42篇 |
1998年 | 319篇 |
1997年 | 198篇 |
1996年 | 100篇 |
1995年 | 65篇 |
1994年 | 40篇 |
1993年 | 64篇 |
1992年 | 7篇 |
1991年 | 9篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 9篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1983年 | 2篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 3篇 |
1978年 | 4篇 |
1977年 | 15篇 |
1976年 | 41篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有1370条查询结果,搜索用时 0 毫秒
61.
62.
Driven by the need for a readily available, non-immunological tissue that possesses many of the characteristics of normal human skin, tissue-engineered skin has been developed. For over a decade, laboratory grown or processed skin has been under investigation and, in some cases, available as an alternative to autologous grafts. Apligraf, derived from neonatal foreskin and bovine type I collagen, is the first bi-layered living skin equivalent approved in the US and other countries for use in venous ulcers. Apligraf is effective both in the treatment of refractory venous ulcers and for acute wounds such as surgical excision sites and split thickness donor sites. Apligraf is safe and is not clinically rejected. Its ultimate fate is not known, so it may well work to aid healing in a variety of ways including graft 'take' and as a stimulus for healing. 相似文献
63.
DJ Pinsky H Liao CA Lawson SF Yan J Chen P Carmeliet DJ Loskutoff DM Stern 《Canadian Metallurgical Quarterly》1998,102(5):919-928
PURPOSE: In a rabbit model, transposition of a muscle pedicle flap to an ischemic hind limb has been shown to result in the development of new blood vessels that connect the arterial circulation of the flap to the circulation of the limb. The hypothesis that exogenous recombinant basic fibroblast growth factor (bFGF) would enhance the development of this new blood supply was examined and the regulation of bFGF in this process was investigated. METHODS: The right common iliac artery was ligated in 12 male New Zealand white rabbits. An abdominal wall muscle flap based on the left inferior epigastric artery was transposed to the right thigh. bFGF in phosphate-buffered saline (PBS) at 3 ng/h (n = 6), or PBS alone (n = 6), was infused for 7 days via mini-osmotic pumps with an infusion catheter positioned at the flap-muscle interface. The flap-muscle interface was immunostained with anti-alpha-actin antibody to determine blood vessel density (number of vessels/mm) and with anti-bFGF antibody to evaluate bFGF distribution. RNA was isolated from these sections, and polymerase chain reaction (PCR) was used to examine endogenous bFGF messenger RNA (mRNA) expression. RESULTS: Blood vessel density was significantly increased in animals receiving exogenous bFGF (22. 0 +/- 10.6 vessels/mm vs. 10.7 +/- 8.8 vessels/mm, P =.009). In the controls, neovessels were arranged in clusters with endogenous bFGF concentrated around these clusters. In bFGF-treated animals, vessels were diffusely scattered throughout the flap-limb interface, corresponding to the distribution pattern of infused bFGF. There was no difference in bFGF mRNA expression between the control and the bFGF-treated groups. CONCLUSION: Exogenous bFGF infusion significantly augmented new blood vessel development at the flap-limb interface. Endogenous bFGF was up-regulated around the newly developed microvessels in control animals, and vessel growth correlated with the diffuse distribution of exogenous bFGF, implicating bFGF as an important factor in angiogenesis. Exogenous bFGF did not affect bFGF mRNA expression, suggesting that the regulation of bFGF is not under autocrine control. 相似文献
64.
65.
SL Cheng SF Zhang S Mohan F Lecanda A Fausto AH Hunt E Canalis LV Avioli 《Canadian Metallurgical Quarterly》1998,71(3):449-458
Müller cells are highly permeable to potassium ions and play a major role in maintaining potassium homeostasis in the vertebrate retina during light-evoked neuronal activity. Potassium fluxes across the Müller cell's membrane are believed to underlie the light-evoked responses of these cells. We studied the potassium currents of turtle Müller cells in the retinal slice and in dissociated cell preparations and their role in the genesis of the light-evoked responses of these cells. In either preparation, the I-V curve, measured under voltage-clamp conditions, consisted of inward and outward currents. A mixture of cesium ions, TEA, and 4-AP blocked the inward current but had no effect on the outward current. Extracellular cesium ions alone blocked the inward current but exerted no effect on the photoresponses. Extracellular barium ions blocked both inward and outward currents, induced substantial depolarization, and augmented the light-evoked responses, especially the OFF component. Exposing isolated Müller cells to a high potassium concentration did not cause any current or voltage responses when barium ions were present. In contrast, application of glutamate in the presence of barium ions induced a small inward current that was associated with a substantially augmented depolarizing wave relative to that observed under control conditions. This observation suggests a role for an electrogenic glutamate transporter in generating the OFF component of the turtle Müller cell photoresponse. 相似文献
66.
SF Abraham JR Blair-West JP Coghlan DA Denton DR Mouw BA Scoggins 《Canadian Metallurgical Quarterly》1976,81(1):120-132
Conscious sheep with permanent indwelling cannulae in the lateral ventricles and the cisterna magna were Na depleted and then perfused for 9 h with an artificial CSF solution. There were 3 experimental groups: Group I (n=5) received perfusion with aritifical CSF containing NA 170 MEq./1, Group II (n=7) received perfusion with artificial CSF containing Na 145 mEq./1, Group III (n=7) received no perfusion. In Group I the blood aldosterone level fell from 26.4 +/- 7.4 to 8.6 +/- 2.3 ng/100 ml by 9 h after perfusion. There was no significant change in plasma [Na] or [K], blood angiotensin II or plasma renin concentration. Blood cortisol and corticosterone levels rose. There was also a fall in post-perfusion. Group III showed no significant change in blood aldosterone concentration. Multivariate statistical analysis showed that the fall in aldosterone levels during 170 mEq./l Na perfusion could not be accounted for by changes, either alone or together, of ACTH as evidenced by alteration in blood cortisol or corticosterone, or by change of plasma [Na], [K] or renin concentrations. This data supports the hypothesis of an additional factor which may be of CNS origin being involved in the control of aldosterone secretion. 相似文献
67.
68.
69.
70.
A G protein gamma subunit-like domain shared between RGS11 and other RGS proteins specifies binding to Gbeta5 subunits 总被引:1,自引:0,他引:1
BE Snow AM Krumins GM Brothers SF Lee MA Wall S Chung J Mangion S Arya AG Gilman DP Siderovski 《Canadian Metallurgical Quarterly》1998,95(22):13307-13312
Regulators of G protein signaling (RGS) proteins act as GTPase-activating proteins (GAPs) toward the alpha subunits of heterotrimeric, signal-transducing G proteins. RGS11 contains a G protein gamma subunit-like (GGL) domain between its Dishevelled/Egl-10/Pleckstrin and RGS domains. GGL domains are also found in RGS6, RGS7, RGS9, and the Caenorhabditis elegans protein EGL-10. Coexpression of RGS11 with different Gbeta subunits reveals specific interaction between RGS11 and Gbeta5. The expression of mRNA for RGS11 and Gbeta5 in human tissues overlaps. The Gbeta5/RGS11 heterodimer acts as a GAP on Galphao, apparently selectively. RGS proteins that contain GGL domains appear to act as GAPs for Galpha proteins and form complexes with specific Gbeta subunits, adding to the combinatorial complexity of G protein-mediated signaling pathways. 相似文献