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91.
J Joris M Ozaki DI Sessler AF Hardy M Lamy J McGuire D Blanchard M Schroeder A Moayeri 《Canadian Metallurgical Quarterly》1994,80(2):268-277
BACKGROUND: The authors tested the hypotheses that: (1) the vasoconstriction threshold during combined epidural/general anesthesia is less than that during general anesthesia alone; and (2) after vasoconstriction, core cooling rates during combined epidural/general anesthesia are greater than those during general anesthesia alone. Vasoconstriction thresholds and heat balance were evaluated under controlled circumstances in volunteers, whereas the clinical importance of intraoperative thermoregulatory vasoconstriction was evaluated in patients. METHODS: Five volunteers were each evaluated twice. On one of the randomly ordered days, epidural anesthesia (approximately T9 dermatomal level) was induced and maintained with 2-chloroprocaine. On both study days, general anesthesia was induced and maintained with isoflurane (0.7% end-tidal concentration), and core hypothermia was induced by surface cooling and continued for at least 1 h after fingertip vasoconstriction was observed. Patients undergoing colorectal surgery were randomly assigned to combined epidural/enflurane anesthesia (n = 13) or enflurane alone (n = 13). In appropriate patients, epidural anesthesia was maintained by an infusion of bupivacaine. The core temperature that triggered fingertip vasoconstriction identified the threshold. RESULTS: In the volunteers, the vasoconstriction threshold was 36.0 +/- 0.2 degrees C during isoflurane anesthesia alone, but significantly less, 35.1 +/- 0.7 degrees C, during combined epidural/isoflurane anesthesia. Cutaneous heat loss and the rates of core cooling were similar 30 min before vasoconstriction with and without epidural anesthesia. In the 30 min after vasoconstriction, heat loss decreased 33 +/- 13 W when the volunteers were given isoflurane alone, but only 8 +/- 16 W during combined epidural/isoflurane anesthesia. Similarly, the core cooling rates in the 30 min after vasoconstriction were significantly greater during combined epidural/isoflurane anesthesia (0.8 +/- 0.2 degrees C/h) than during isoflurane alone (0.2 +/- 0.1 degrees C/h). In the patients, end-tidal enflurane concentrations were slightly, but significantly, less in the patients given combined epidural/enflurane anesthesia (0.6 +/- 0.2% vs. 0.8 +/- 0.2%). Nonetheless, the vasoconstriction threshold was 34.5 +/- 0.6 degrees C in the epidural/enflurane group, which was significantly less than that in the other patients, 35.6 +/- 0.8 degrees C. When the study ended after 3 h of anesthesia, patients given combined epidural/enflurane anesthesia were 1.2 degrees C more hypothermic than those given general anesthesia alone. The rate of core cooling during the last hour of the study was 0.4 +/- 0.2 degrees C/h during combined epidural/enflurane anesthesia, but only 0.1 +/- 0.3 degrees C/h during enflurane alone. CONCLUSIONS: These data indicate that epidural anesthesia reduces the vasoconstriction threshold during general anesthesia. Furthermore, the markedly reduced rate of core cooling during general anesthesia alone illustrates the importance of leg vasoconstriction in maintaining core temperature. 相似文献
92.
R Arumugam HE Soriano AO Scheimann BS Reid GS Gopalakrishna O Barakat CF Ozaki PR Wood 《Canadian Metallurgical Quarterly》1998,12(6):588-592
Lipopolysaccharide (LPS) from S. typhimurium on exposure to gamma-radiation resulted in decrease in toxicity and was less mitogenic, Silver stained profiles of irradiated LPS on polyacrylamide gels revealed complete loss of its heteropolysaccharides which was confirmed further by analysing lipid A and LPS from Salmonella minnesota Re mutants on SDS-PAGE. Glucosamine and 2-keto 3-deoxy-octonate(Kdo) contents were significantly decreased on treatment. Lipid A obtained by removal of heteropolysaccharides from LPS was less toxic on exposure to gamma radiations. 相似文献
93.
Ozaki T. Suzuki T. Furuhashi T. Okuma S. Uchikawa Y. 《Industrial Electronics, IEEE Transactions on》1991,38(3):195-202
The authors present a nonlinear compensator using neural networks for trajectory control of robotic manipulators. The neural networks are not used to learn inverse-dynamics but to compensate nonlinearities of robotic manipulators. The performance of the proposed neural network controller is compared with that of the adaptive controller proposed by J.J. Craig (1988), and the effectiveness of the proposed neural network controller in compensating the unstructured uncertainties is clarified. A learning scheme using a model of known dynamics of manipulators is also proposed. The model learning can be done offline and needs no data recording of actual manipulator operation 相似文献
94.
Thermally induced structural changes of polyethylene (PE) have been studied by means of near-infrared (NIR) spectroscopy in the course of heating up to the melting temperature. NIR bands characteristic of the regular orthorhombic phase, the conformationally disordered hexagonal phase, and the amorphous phase have been successfully identified. It has been found that for the unoriented PE sample, the disordering process of orthorhombic lattice starts above room temperature and that it mostly occurs above 100 °C for the uniaxially oriented PE sample. In the latter case, the enhancement of crystallinity has clearly been detected just below Tm due to the reorganization of crystalline lattice. For the geometrically constrained ultradrawn PE sample, the phase transition from orthorhombic to hexagonal phase has been detected immediately below the melting point. The NIR bands characteristic of the hexagonal phase have been confirmed definitely. Usefulness of NIR spectroscopy has been demonstrated successfully in such a study of thermally induced phase transition behavior of PE samples with appreciable thickness, for which mid-IR spectroscopy is difficult to apply because of the intensity saturation of various key bands. 相似文献
95.
Phosphorus removal under anoxic conditions in a continuous-flow A2N two-sludge process. 总被引:2,自引:0,他引:2
The Anaerobic-Anoxic/Nitrification (A2N) system is a continuous-flow, two-sludge process in which Poly-P bacteria are capable of taking up phosphate under anoxic conditions using nitrate as an electron acceptor. The process is very efficient because it maximizes the utilization of organic substrate for phosphorus and nitrogen removal. An experimental lab-scale A2N system fed with domestic sewage was tested over a period of 260 days. The purpose of the experiment was to examine phosphorus removal capacity of a modified A2N two-sludge system. Factors affecting phosphorus and nitrogen removal by the A2N system were investigated. These factors were the influent COD/TN ratio, Sludge Retention Time (SRT), Bypass Sludge Flow rate (BSF) and Return Sludge Flow rate (RSF). Results indicated that optimum conditions for phosphorus and nitrogen removal were the influent COD/TN ratio around 6.49, the SRT of 14 days, and the BSF and RSF were fixed at about 26-33% of influent flow rate. 相似文献
96.
T Kanaji H Ozaki T Takao H Kawajiri H Ide M Motoki Y Shimonishi 《Canadian Metallurgical Quarterly》1993,268(16):11565-11572
The complete amino acid sequence of transglutaminase (EC 2.3.2.13) (TGase), which is produced by a microorganism, Streptoverticillium sp. strain s-8112, and catalyzes the acyl transfer reaction between gamma-carboxyamide groups of glutamine residues in proteins and various primary amines, has been established by a combination of fast atom bombardment mass spectrometry and standard Edman degradation of peptide fragments produced by treatment of the TGase with various proteolytic enzymes and purified by a reversed-phase high performance liquid chromatography. The TGase consists of 331 amino acid residues with a chemical molecular weight of 37,863, in agreement with the observed molecular weight (37,869.2 +/- 8.8) determined from its electrospray ionization mass spectrum. The sequence of the enzyme is very different from those of mammalian TGases represented by guinea pig liver enzyme. The enzyme contains a sole Cys residue, which is essential for its catalytic activity. Hydropathy analysis indicated that the secondary structure of the region around the active site Cys residue is similar to those of mammalian TGases. These results suggest that this microbial protein evolved by a different pathway from that of mammalian TGases and acquired acyl transfer activity during the evolutional process. 相似文献
97.
J Lappalainen JC Long M Eggert N Ozaki RW Robin GL Brown H Naukkarinen M Virkkunen M Linnoila D Goldman 《Canadian Metallurgical Quarterly》1998,55(11):989-994
We examined the effects of repeated administration of (S)-alpha-fluoromethylhistidine (FMH), a specific inhibitor of histidine decarboxylase (HDC), on radial maze performance and brain contents of histamine and amino acids in rats. By daily subcutaneous (s.c.) administration of FMH (100 mg/kg), rats showed significant enhancement of a radial maze performance without changes in locomotion. Six days after FMH treatment, the histamine levels both in the cerebral cortex and diencephalon decreased significantly. However, the glutamate and glycine levels significantly increased in the cerebral cortex and hippocampus. These results suggest that FMH enhances the acquisition phase of radial maze study with the increases in glutamate and glycine levels in the cerebral cortex and hippocampus of rats. 相似文献
98.
In bovine tracheal smooth muscle, carbachol (CCh, 1 microM) and high K+ (72.7 mM) induced sustained increases in cytosolic Ca2+ level ([Ca2+]i), myosin light chain (MLC) phosphorylation and force of contraction. Forskolin (FK, 1-10 microM) inhibited the CCh-induced increase in [Ca2+]i, MLC phosphorylation and force in parallel. In contrast, FK inhibited the high K(+)-induced contraction and MLC phosphorylation without changing [Ca2+]i. In the absence of extracellular Ca2+ (with 0.5 mM EGTA), CCh (10 microM) and caffeine (20 mM) induced transient increase in [Ca2+]i and contractile force by releasing Ca2+ from cellular store. FK strongly inhibited the CCh-induced Ca2+ transient, but failed to inhibit the caffeine-induced Ca2+ transient. In the absence of external Ca2+, 12-deoxyphorbol 13-isobutylate (DPB, 1 microM) induced sustained contraction without increase in [Ca2+]i and MLC phosphorylation. FK inhibited this contraction without changing [Ca2+]i. In permeabilized muscle, Ca2+ induced contraction in a concentration-dependent manner. FK (10 microM) and cAMP (1-100 microM) shifted the Ca(2+)-force curve to the higher Ca2+ levels. CCh with GTP, GTP gamma S or DPB enhanced contraction in the presence of constant level of Ca2+. Forskolin and cAMP also inhibited the enhanced contractions in the permeabilized muscle. In the permeabilized, thiophosphorylated muscle, ATP induced contraction in the absence of Ca2+. cAMP (300 microM) had no effect on this contraction. These results suggest that forskolin inhibits agonist-induced contraction in tracheal smooth muscle by multiple mechanisms of action; 1) inhibition of MLC phosphorylation by reducing Ca2+ influx and Ca2+ release, 2) inhibition of MLC phosphorylation by changing the MLC kinase/phosphatase balance, and 3) inhibition of regulatory mechanism which is not dependent on MLC phosphorylation. 相似文献
99.
This paper proposes a radiographic sensitivity attenuation curve for screen-films systems. This curve expresses the relative inverse value of mAs value needed to give the same photographic density. This curve decreases more slowly with increasing object thickness than the exposure attenuation curve generally used hitherto. By using the radiographic sensitivity attenuation curve and the exposure attenuation curve, the voltage-ripple dependence of mAs values and patient dose for the same photographic density was obtained. As predicted theoretically in a previous paper [Med. Phys. 18, 921 (1991)], it was verified quantitatively the reverse order that larger ripple values resulted in lower patient dose and lower mAs value for the unsaturating current mode at large ripple. This reverse order clearly appeared in two-pulse units. These phenomena were experimentally verified. As a result it was found that in radiographing with a two-pulse unit, larger mA value results in lower patient dose. 相似文献
100.