CDK4 down-regulation induced by paclitaxel is associated with G1 arrest in gastric cancer cells

Paclitaxel induces a cell cycle block at G2-M phase by preventing the depolymerization of microtubules and induces p53-independent apoptosis in many cancer cells. We observed that gastric cancer cells treated with paclitaxel have shown a cyclin-dependent kinase (CDK)4 down-regulation. This paclitaxel-induced CDK4 down-regulation resulted in a cell cycle arrest at G1-S phase. To confirm this observation, we prepared stable transfectants that overexpressed CDK4 and analyzed the cell cycle progression. Ectopic expression of CDK4 in SNU cells resulted in a release of paclitaxel-induced G1 arrest. The release of G1 arrest by enforced expression of CDK4 seems to make the cells more sensitive to paclitaxel-induced apoptosis. From this finding, we could then suggest that paclitaxel treatment induces both G1-S and G2-M blocks in the cell cycle progression of gastric cancer cells.     

Novel sequence organization and insertion specificity of IS605 and IS606: chimaeric transposable elements of Helicobacter pylori

We have used PKH26 dye, which is incorporated stably into the membrane of cells, to determine, using flow cytometry, lymphocyte proliferative responses to the antigen tetanus toxoid in fresh and cryopreserved samples. Measuring cell proliferation with this dye has advantages over either 3H-thymidine or Bromodeoxyuridine (BrdU). Whereas the existing methods measure proliferation at a single time point, PKH26 gives a cumulative measure of cell proliferation. As PKH26 is incorporated into the cell membrane, cells do not have to be permeabilised to allow dye incorporation into a cytoplasmic compartment. Most importantly, PKH26 can be used in combination with monoclonal antibodies to surface markers on mixed populations of cells, to determine the proliferation of individual subpopulations, without the need for prior cell fractionation. We also show that PKH26 can be used with similar efficacy in both fresh and cryopreserved samples. In addition since PKH26 is a cumulative measure of proliferative responses we were able to show that restimulation of the dividing population in vitro with fresh antigen presenting cells (APC) and antigen permits characterisation of a further proliferating cell population. The use of PKH26 dye in combination with cell phenotyping and measurement of cytokine production at the single cell level will prove a powerful tool for multiparameter analyses of cellular responses to antigen.     

直齿轮渗碳层厚度分布与组织改善技术途径

针对传统的“先成形，后渗碳”工艺在硬齿面齿轮渗碳层厚度分布和组织形态方面存的问题，提出齿轮“渗碳一温挤”成形新工艺。实验结果表明，通过调整模具结构(模具圆角半径等)、合理选择成形工艺参数(成形温度等)，可使齿轮渗碳层厚度分布合理、组织改善(晶粒细化)。     

Transgenic models of Huntington's disease

CAG/polyglutamine expansion has been shown to form the molecular basis of an increasing number of inherited neurodegenerative diseases. The mutation is likely to act by a dominant gain of function but the mechanism by which it leads to neuronal dysfunction and cell death is unknown. The proteins harbouring these polyglutamine tracts are unrelated and without exception are widely expressed with extensively overlapping expression patterns. The factors governing the cell specific nature of the neurodegeneration have yet to be understood. Upon a certain size threshold, expanded CAG repeats become unstable on transmission and a modest degree of somatic mosaicism is apparent. Similarly, the molecular basis of the instability and its tissue specificity has yet to be unravelled. Recent reports describing the first mouse models of CAG/polyglutamine disorders indicate that it will be possible to model both the pathogenic mechanism and the CAG repeat instability in the mouse. This has great potential and promise for uncovering the molecular basis of these diseases and developing therapeutic interventions.     

Carcinogenic polycyclic aromatic hydrocarbons increase intracellular Ca2+ and cell proliferation in primary human mammary epithelial cells

A method was developed to determine in eggs 2 components [4,6-dimethyl-2-hydroxypyrimidine and 1,3-bis(4-nitrophenyl)urea] of the anticoccidial drug nicarbazin, used to treat poultry. Samples were extracted with acetonitrile, and the extracts were washed with hexane and evaporated to dryness before analysis by liquid chromatography/mass spectrometry with atmospheric pressure chemical ionization. By switching from positive to negative ion monitoring and using gradient elution, both components were measured within one run. The limit of quantitation of the assay was 10 ng/g for each component. The results of a preliminary feeding trial in which chickens were fed contamination levels of the drug are also reported.     

Reduction of triceps muscle force after shortening of the distal humerus: a computational model

A retrospective case reference study examining the use of alcohol and tobacco in 303 women aged 40 or over suffering from oral or oropharyngeal cancer was conducted in the south-west Netherlands. Both alcohol and tobacco consumption are important in the development of oral and oropharyngeal cancer with increased consumption of both markedly increasing the risks of cancer, but alcohol having the greater effect.     

A method to convert AVHRR Normalized Difference Vegetation Index time series to a standard viewing and illumination geometry

The bi-directional reflectance distribution function (BRDF) alters the seasonal and inter-annual variations exhibited in Advanced Very High Resolution Radiometer (AVHRR) Normalized Difference Vegetation Index (NDVI) data and this hampers the detection and, consequently, the interpretation of temporal variations in land-surface vegetation. The magnitude and sign of bi-directional effects in commonly used AVHRR data sets depend on land-surface properties, atmospheric composition and the type of atmospheric correction that is applied to the data. We develop an approach to estimate BRDF effects in AVHRR NDVI time series using the Moderate Resolution Imaging Spectrometer (MODIS) BRDF kernels and subsequently adjust NDVI time series to a standard illumination and viewing geometry. The approach is tested on NDVI time series that are simulated for representative AVHRR viewing and illumination geometry. These time series are simulated with a canopy radiative transfer model coupled to an atmospheric radiative transfer model for four different land cover types—tropical forest, boreal forest, temperate forest and grassland - and five different atmospheric conditions - turbid and clear top-of-atmosphere, turbid and clear top-of-atmosphere with a correction for ozone absorption and Rayleigh scattering applied (Pathfinder AVHRR Land data) and ground-observations (fully corrected for atmospheric effects). The simulations indicate that the timing of key phenological stages, such as start and end of growing season and time of maximum greenness, is affected by BRDF effects. Moreover, BRDF effects vary with latitude and season and increase over the time of operation of subsequent NOAA satellites because of orbital drift. Application of the MODIS kernels on simulated NVDI data results in a 50% to 85% reduction of BRDF effects. When applied to the global 18-year global Normalized Difference Vegetation Index (NDVI) Pathfinder data we find BRDF effects similar in magnitude to those in the simulations. Our analysis of the global data shows that BRDF effects are especially large in high latitudes; here we find that in at least 20% of the data BRDF errors are too large for accurate detection of seasonal and interannual variability. These large BRDF errors tend to compensate, however, when averaged over latitude.     

步进电机自动升降速闭环控制的单片机实现

本文提出了一种步进电机闭环控制原理。根据这一原理 ,介绍了由单片机实现对步进电机自动升降速闭环控制的方法。     

200MW 机组高压加热器给水大旁路经济性分析

目前国产高参数大容量机组的高压加热器均采用给水大旁路系统。从节能角度出发,以国产200MW 机组为例,采用等效焓降法,对高压加热器给水系统采用大旁路或小旁路进行了热经济性对比分析。若用小旁路系统,计算表明,一台200MW 机组按目前运行工况可节约标煤2112t。