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221.
Strength of Materials - Creep constitutive equations have been derived for the materials that exhibit the properties of orthotropy (transversal isotropy) and transient creep under cyclic loading. A...  相似文献   
222.
Chorismate and isochorismate constitute branch-point intermediates in the biosynthesis of many aromatic metabolites in microorganisms and plants. To obtain unnatural compounds, we modified the route to menaquinone in Escherichia coli. We propose a model for the binding of isochorismate to the active site of MenD ((1R,2S, 5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxycyclohex-3-ene-1-carboxylate (SEPHCHC) synthase) that explains the outcome of the native reaction with α-ketoglutarate. We have rationally designed variants of MenD for the conversion of several isochorismate analogues. The double-variant Asn117Arg–Leu478Thr preferentially converts (5S,6S)-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylate (2,3-trans-CHD), the hydrolysis product of isochorismate, with a >70-fold higher ratio than that for the wild type. The single-variant Arg107Ile uses (5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylate (2,3-trans-CHA) as substrate with >6-fold conversion compared to wild-type MenD. The novel compounds have been made accessible in vivo (up to 5.3 g L−1). Unexpectedly, as the identified residues such as Arg107 are highly conserved (>94 %), some of the designed variations can be found in wild-type SEPHCHC synthases from other bacteria (Arg107Lys, 0.3 %). This raises the question for the possible natural occurrence of as yet unexplored branches of the shikimate pathway.  相似文献   
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224.
Journal of Communications Technology and Electronics - Approximate formulas for calculating the coefficient of reflection from an artificial magnetic conductor (AMC) based on a capacitive lattice...  相似文献   
225.
An effective practical approach that allows not only a significant reduction in the scope of practical experiments in the course of studying suspension separation processes in hydrocyclones, but also makes it possible to assess the intensity of random components of the processes and define the interrelation between such components and hydrodynamics of flows in a hydrocyclone is presented. Within the frames of the developed probabilistic‐statistical model of suspension separation in hydrocyclones on the basis of statistical self‐similarity properties, a relationship was found between determined and random components of the processes. This allowed transitioning from three‐parameter probability density functions for suspension particles in hydrocyclones to two‐parameter functions; thus significantly improving the efficiency of practical application of the developed model.  相似文献   
226.
Power Technology and Engineering - The monitoring of hydraulic structures as high-risk facilities is discussed. Monitoring should ensure the reliability and functioning of a hydraulic structure...  相似文献   
227.
Theoretical Foundations of Chemical Engineering - A choline chloride–sulfosalicylic acid deep eutectic solvent (DES) is proposed for dissolving the oxides Co(II), Cu(II), Zn(II), Fe(III),...  相似文献   
228.
Atomic Energy - The results of calculations and experimental determination of the neutronics characteristics of the IRT-T research reactor are presented. The IRT-T reactor is a pool reactor with...  相似文献   
229.
Protection of Metals and Physical Chemistry of Surfaces - In this work, a universal sorbent has been obtained that works according to the mechanisms of chemisorption and adsorption by impregnating...  相似文献   
230.
Journal of Communications Technology and Electronics - Microstrip band-pass filters (BPFs) containing quarter-wavelength resonators and a Π-shaped half-wavelength resonator placed next to each...  相似文献   
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