SEM-EDX analysis in the source apportionment of particulate matter on Hypogymnia physodes lichen transplants around the Cu smelter and former mining town of Karabash, South Urals, Russia

Scanning electron microscopy with energy-dispersive X-ray analysis (SEM-EDX) of particulate matter on lichen transplant thalli (Hypogymnia physodes) was assessed as a complementary technique to wet chemical analysis for source apportionment of airborne contaminants. Transplants (2 month exposure) stationed in the Cu smelter and former mining town of Karabash were compared with those from a control site 30 km south. Particulate matter in Karabash samples (715 analyses) showed higher levels of S, Pb, Cu, Sn and Zn compared with the control (598 analyses). Complex element associations among the particles confounded detailed mineralogical identifications, and therefore a simplified particle classification scheme was devised for source apportionment. Karabash samples contained high levels of particles classified as mining-related (MRP), and these were also identified in control samples, indicating wide spatial dispersion from the smelter and highlighting the sensitivity of the method. It was noted that MRP <2.5-microm diameter were poorly represented on lichen surfaces suggesting this may limit the usefulness of Hypogymnia transplants as proxies when assessing human health impacts from airborne particulates. Analyses of the lichen thallus surface (away from surface particulates) revealed high levels of Cu, Zn, Fe and Pb associated with organics in the Karabash samples compared with the control, with a proportionate loss of K, interpreted as being due to a stress-related increase in cell membrane permeability. This type of analysis may provide a novel SEM-EDX-based method for assessing lichen vitality. The techniques developed are presented and further implications of the study are discussed.     

Biofilm quantification on stone surfaces: comparison of various methods

In the present study, the efficacy of three different methods of estimating biofilm biomass on stone (amount of chlorophyll a, fluorescein diacetate hydrolysis and changes in colour) is discussed. Stone samples were sprayed with solutions of cyanobacteria and the actual microbial biomass--determined by cell culture--was compared with the biomass estimated using the different methods. Statistically significant differences between actual biomass and that estimated from the fluorescein diacetate hydrolysis method invalidate its use. However, although the results indicated the amount of chlorophyll to be a good estimator of biofilm biomass, problems in the total extraction of chlorophyll from the stone cannot be overlooked. Determination of changes in colour was found to be a good method of quantifying biofilms growing on stone.     

Assessment of environmental tobacco smoke exposure: urinary cotinine concentrations in children are strongly associated with the house dust concentrations of nicotine at home

In the present study the possibility of using nicotine in house dust as an index of environmental tobacco smoke (ETS) exposure was evaluated in an environmental investigation of 23 children with asthma. A standardized procedure for house dust sampling of nicotine with a filter holder connected to a vacuum cleaner, for a defined time and area was developed (F-nicotine). Also, house dust sampling was carried out from the vacuum cleaner bags of the homes (VC-nicotine). There was a larger variation in VC-nicotine (13-655, median 66 microg/g) compared with F-nicotine (15-393 median 156 microg/g). There were statistically significant associations between an inquiry data based ETS exposure index on the one hand, and urinary cotinine concentrations in children (U-cotinine), F-nicotine and VC-nicotine of their homes, on the other. The strong correlation between U-cotinine and F-nicotine (rs = 0.93; P < 0.0001) indicates that the new standardized house dust sampling method should be useful in ETS exposure assessment. However, further validation by a larger sample size with repeated measurements in the same homes is needed.     

Dissolution of copper, tin, and iron from sintered tungsten-bronze spheres in a simulated avian gizzard, and an assessment of their potential toxicity to birds

The rates of dissolution of copper, tin, and iron from sintered tungsten-bronze spheres (51.1%W, 44.4%Cu, 3.9%Sn, 0.6%Fe, by mass) were measured in an in vitro simulated avian gizzard at pH 2.0, and 42C. Most of the spheres had disintegrated completely to a fine powder by day 14. Dissolution of copper, tin, and iron from the spheres was linear over time; all r>0.974; all P<0.001. The mean rate of release of copper, tin, and iron was 30.4 mg, 2.74 mg, and 0.38 mg per g tungsten-bronze per day, respectively. These rates of metal release were compared to those in published studies to determine whether the simultaneous ingestion of eight spheres of 3.48 mm diameter would pose a toxic risk to birds. The potential absorption rates of iron and tin (0.54 mg Fe/day, and 3.89 mg Sn/day) from eight tungsten-bronze spheres of total mass 1.42 g would not prove toxic, based on empirical studies of tin and iron ingestion in waterfowl. The release of 43.17 mg copper/day from eight tungsten-bronze spheres, while exceeding the daily copper requirements of domesticated birds, is far below the levels of copper known to cause copper toxicosis in birds. We conclude that sintered tungsten-bronze material made into gunshot, fishing weights, or wheel balance weights, would not pose a toxic risk to wild birds when ingested.     

Haloacetic acids and trihalomethanes in finished drinking waters from heterogeneous sources

Trihalomethanes (THM) and haloacetic acids (HAA) are the most frequent chlorination by-products (CBP) in finished drinking waters. Traditionally, THM have been used as surrogates for CBP although the quantitative association between THM and other CBP is not well established. This problem is addressed in the present study from the analysis of THM and HAA in drinking water samples from four Spanish regions, representing areas with very different CBP composition, e.g. between 86 and 8.0 microg/l of THM and 50-3.0 microg/l of HAA.The resulting dataset exhibit a statistically significant correlation between total THM and HAA (Pearson's correlation coefficient, r(p)=0.815,p<0.0005). Furthermore, specific HAA are highly correlated with specific THM or their combinations. Accordingly, multivariate linear regression analysis of the concentrations observed show that the levels in total and specific HAA can be predicted from the THM content. These results are relevant for epidemiological studies on health effects from CBP exposure since they usually involve comparison of populations consuming waters of very distinct quality.     

The acute effect of bunker C oil and an oil dispersant on: 1 Serum glucose, serum sodium and gill morphology in both freshwater and seawater acclimated rainbow trout (Salmo gairdneri)

Bunker C oil and an oil dispersant were tested for physiological stress on both freshwater and saltwater acclimated rainbow trout. Both compounds tended to reduce serum glucose levels with Bunker C causing the more significant decrease (P < 0.08), indicating a possible dysfunction of the kidney. The freshwater treatment group showed a significant decrease in sodium levels (P < 0.01) when treated with a dispersant, while under similar conditions, saltwater acclimated fish show a very marked increase in serum sodium concentrations (P < 0.025). Those fluctuations in sodium levels are resultant from direct interference with the energy activated sodium transport systems of the gills. Microphotographs of gill filaments and lamellae show severe damage caused by the dispersant and dispersant/oil mixture with less impairment resultant from Bunker C exposure.     

Does oyster size matter for modelling trace metal bioaccumulation?

The aim of this study was assessing whether trace metal bioaccumulation models for oysters need to account for the effect of size-structured demography. To achieve this, we used a Bayesian generalised additive mixed modelling (BGAMM) approach. This nonparametric regression approach allowed us to estimate potentially nonlinear size effects of the oysters as well as random and structured spatial effects. Native oysters were collected from ten locations around the shoreline of Moreton Bay, a subtropical estuary in southeast Queensland, Australia. The soft-tissue of these sampled oysters were weighed (dry weight basis) and then analysed for Al, Cu, Fe, Mg, Mn and Zn. Of the six trace metals analysed, only Mg was found to have a significant (negative) effect of soft-tissue mass. The correlated spatial effects for Cu, Mn and Zn indicated that the source of these trace metals in the oysters was from anthropogenic inputs while the correlated spatial effects for Al and Mg indicated natural inputs. The correlated spatial effect for Fe was characterised by 'hotspot' concentrations in regions of Moreton Bay where algal blooms have previously occurred and consequently oysters might be useful as an indicator of algal blooms in the Bay. Overall, the absence of an observed size effect for five of the six trace metals indicates that bioaccumulation models do not necessarily need to account for size-structured demography therefore enabling simpler models to be used.     

The effect of depositional history on contaminated bed sediment stability

Experiments were conducted in an annular flume using a commercially available kaolinite clay as well as contaminated bed sediment from Hamilton Harbour (Ontario) to assess their stability against erosion. Critical shear stress for erosion was measured under different conditions of bed formation (quiescently deposited beds and shear deposited beds) as well as with and without the presence of a biostabilized bed. Results suggest that a biostabilized bed and a bed formed under a flowing condition, similar to a river scenario, will be more resistant against erosion than will a non-biostabilized bed and a bed formed under quiescent conditions. Up to three cycles of erosion and flocculation/deposition were observed to occur within one experiment. These results suggest that the depositional history and biostabilization of river bed sediments need to be seriously considered within sediment and contaminant transport models if meaningful estimates of sediment and contaminant source, fate and effect are to be generated and used for the management of our aquatic ecosystems.     

Analysis of point load test as a method for preliminary geotechnical classification of rocks

This paper deals with the analysis of the point load compression test made on irregular-shaped rock fragments, proposed in the late 50’s by Protodiakonov. An ample revision of the literature on the subject has been made, and a series of tests, using seven different types of rock materials, has been developed. The results obtained from these tests showed that parameters generally used in literature to relate the unconfined compressive (or tensile) strength of materials, resulting from point load tests, can lead to scattered results, due mainly to the scale effect, of great evidence in such tests. It seems possible to obtain compressive (and tensile) strength of rocks, if the ratio between failure load and the distance between load application poins, with a 3/2 exponent, is considered, in field work, as being the "point load strength index".

---

Resume L’article présente une analyse critique de l’essai de compression sur échantillons irréguliers de roche, proposé par Protodiakonov vers la fin des années 50. Tout d’abord on a fait une révision de la littérature disponible sur le sujet et, après, une série d’essais sur sept types différents de matériaux. Les résultats obtenus ont montré que les paramètres plus utilisés, dans la littérature, pour définir la résistance à la compression (ou traction) des roches, d’après les essais ponctuels, peuvent provoquer une dispersion très importante des résultats. Cependant, il nous semble valable de suggérer la possibilité de déterminer, sur le chantier, la résistance à la compression (ou traction) des roches, si l’on considère le quotient entre la charge de rupture et la distance entre les deux points d’application de cette charge, le dénominateur à la puissance 3/2, comme "l’indice de charge ponctuelle".

     

Chemical and physical characteristics of a eutrophic reservoir and its tributaries: Long Lake, Washington

Surveillance on the Spokane River and its tributaries has shown that a major source of nutrients influencing the eutrophication of Long Lake was the effluent of the Spokane primary sewage treatment plant. Existing concentrations of nitrate nitrogen and orthophosphate increased threefold and tenfold, respectively, below the effluent. Of the influent phosphate phosphorus, 19 per cent was retained in the reservoir.A density current system was evident in Long Lake which altered vertical and longitudinal distribution of physical and chemical parameters. This current isolated a wedge of water on the reservoir bottom which became anaerobic. During the period of anaerobiosis, phosphate phosphorus and nitrogen maxima were present in the hypolimnion.