Role of miRNAs in Human T Cell Leukemia Virus Type 1 Induced T Cell Leukemia: A Literature Review and Bioinformatics Approach

Human T cell leukemia virus type 1 (HTLV-1) was identified as the first pathogenic human retrovirus and is estimated to infect 5 to 10 million individuals worldwide. Unlike other retroviruses, there is no effective therapy to prevent the onset of the most alarming diseases caused by HTLV-1, and the more severe cases manifest as the malignant phenotype of adult T cell leukemia (ATL). MicroRNA (miRNA) dysfunction is a common feature of leukemogenesis, and it is no different in ATL cases. Therefore, we sought to analyze studies that reported deregulated miRNA expression in HTLV-1 infected cells and patients’ samples to understand how this deregulation could induce malignancy. Through in silico analysis, we identified 12 miRNAs that stood out in the prediction of targets, and we performed functional annotation of the genes linked to these 12 miRNAs that appeared to have a major biological interaction. A total of 90 genes were enriched in 14 KEGG pathways with significant values, including TP53, WNT, MAPK, TGF-β, and Ras signaling pathways. These miRNAs and gene interactions are discussed in further detail for elucidation of how they may act as probable drivers for ATL onset, and while our data provide solid starting points for comprehension of miRNAs’ roles in HTLV-1 infection, continuous effort in oncologic research is still needed to improve our understanding of HTLV-1 induced leukemia.     

Jack-Bean Starch. 1. Properties of the Granules and of the Pastes

Jack bean (Canavalia ensiformis) starch is composed of granules measuring up to 37 micra. Gelatinization temperatures of the granules range from 67.5 to 78 °C. Its pastes, in concentrations up to 6 % did not show swelling peak which appears only in high concentrations. They are very stable during stirring, both at low and high temperatures. They are also more resistant to α-amylase bacterial action than those of corn starch. The results, both for granules and pastes of this starch, show a great resemblance with the same data for guandu bean.     

Effects of Low-Intensity and Long-Term Aerobic Exercise on the Psoas Muscle of mdx Mice: An Experimental Model of Duchenne Muscular Dystrophy

Duchenne muscular dystrophy (DMD) is a muscle disease characterized by the absence of the protein dystrophin, which causes a loss of sarcolemma integrity, determining recurrent muscle injuries, decrease in muscle function, and progressive degeneration. Currently, there is a need for therapeutic treatments to improve the quality of life of DMD patients. Here, we investigated the effects of a low-intensity aerobic training (37 sessions) on satellite cells, peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1α protein (PGC-1α), and different types of fibers of the psoas muscle from mdx mice (DMD experimental model). Wildtype and mdx mice were randomly divided into sedentary and trained groups (n = 24). Trained animals were subjected to 37 sessions of low-intensity running on a motorized treadmill. Subsequently, the psoas muscle was excised and analyzed by immunofluorescence for dystrophin, satellite cells, myosin heavy chain (MHC), and PGC-1α content. The minimal Feret’s diameters of the fibers were measured, and light microscopy was applied to observe general morphological features of the muscles. The training (37 sessions) improved morphological features in muscles from mdx mice and caused an increase in the number of quiescent/activated satellite cells. It also increased the content of PGC-1α in the mdx group. We concluded that low-intensity aerobic exercise (37 sessions) was able to reverse deleterious changes determined by DMD.     

Monitoring of ochratoxin A exposure of the Portuguese population through a nationwide urine survey — Winter 2007

Ochratoxin A (OTA) is a mycotoxin produced by a variety of fungi, such as Penicillium verrucosum and Aspergillium spp., which has been found to have a wide number of potentially deadly toxic effects, and can enter the human organism through a variety of means. It then finds its way into the bloodstream and, after a lengthy process, is eventually excreted through the urine. It can thus be detected in its original form not only in blood samples but also in this biological medium. As such, and in an attempt to evaluate the exposure of the Portuguese population to this mycotoxin, morning urine samples were collected during the Winter of 2007, from each of five geographically distinct Portuguese locations — Bragança, Porto, Coimbra, Alentejo, and Algarve — and subjected to extraction by immunoaffinity columns and to OTA quantification through liquid chromatography coupled with fluorescence detection. Prevalent incidence was higher than 95% with Coimbra being the exception (incidence of 73.3%). In nearly all locations, the OTA content of most samples was found to be above the limit of quantification (LOQ) of 0.008 ng/ml. Indeed, excluding Coimbra, with an OTA content level of 0.014 ng/ml, all regions featured content values over 0.021 ng/ml.     

Low voltage low power fully differential self‐biased 1.5‐bit quantizer with built‐in thresholds

A new fully differential self‐biased 1.5‐bit flash quantizer with built‐in threshold voltages, suitable for high speed ADCs and low voltage operation, is described. The proposed circuit is very simple, and simulation results in a 65 nm standard CMOS technology demonstrate that, following the suggested design methodology, it is able to achieve low offset, low kickback noise, low metastability probability errors and fast regeneration time with very low power dissipation. Copyright © 2011 John Wiley & Sons, Ltd.     

The Effect of Mismatches and Delay on the Quadrature Error of a Cross-Coupled Relaxation Oscillator

Cross-coupled relaxation oscillators can produce two highly accurate quadrature output signals (Verhoeven, 1992). We present a high-level model of these oscillators in terms of circuit parameters, from which we obtain explicit equations for duty-cycle, oscillation frequency, and quadrature error. They show the influence on the oscillator performance of component mismatches and other nonideal effects, such as delays. The results provide useful guidelines for the design of high performance oscillators. The theoretical results are confirmed by simulation and by measurements on a test chip.     

A tree‐based algorithm for virtual infrastructure allocation with joint virtual machine and network requirements

Cloud providers have introduced the on‐demand provisioning of virtual infrastructures (VIs) to deliver virtual networks of computing resources as a service. By combining network and computing virtualization, providers allow traffic isolation between hosted VIs. Taking advantage of this opportunity, tenants have deployed private VIs with application‐optimized network topologies to increase quality of experience of final users. One of the main open challenges in this scenario is the allocation of physical resources to host VIs in accordance with quality of service computing (eg, virtual CPUs and memory) and network requirements (guaranteed bandwidth and specific network topology). Moreover, a VI can be allocated anywhere atop a network datacenter, and because of its NP‐hard complexity, the search for optimal solutions has a limited applicability in cloud providers as requesting users seek an immediate response. The present work proposes an algorithm to accomplish the VI allocation by applying tree‐based heuristics to reduce the search space, performing a joint allocation of computing and network resources. So as to accomplish this goal, the mechanism includes a strategy to convert physical and virtual graphs to trees, which later are pruned by a grouped accounting algorithm. These innovations reduce the number of comparisons required to allocate a VI. Experimental results indicate that the proposed algorithm finds an allocation on feasible time for different cloud scenarios and VI topologies, while maintaining a high acceptance rate and a moderate physical infrastructure fragmentation.     

Analog circuit synthesis performing fast Pareto frontier exploration and analysis through 3D graphs

This paper presents a technique for performing analog design synthesis at circuit level providing feedback to the designer through the exploration of the Pareto frontier. A modified simulated annealing which is able to perform crossover with past anchor points when a local minimum is found which is used as the optimization algorithm on the initial synthesis procedure. After all specifications are met, the algorithm searches for the extreme points of the Pareto frontier in order to obtain a non-exhaustive exploration of the Pareto front. Finally, multi-objective particle swarm optimization is used to spread the results and to find a more accurate frontier. Piecewise linear functions are used as single-objective cost functions to produce a smooth and equal convergence of all measurements to the desired specifications during the composition of the aggregate objective function. To verify the presented technique two circuits were designed, which are: a Miller amplifier with 96 dB Voltage gain, 15.48 MHz unity gain frequency, slew rate of 19.2 V/μs with a current supply of 385.15 μA, and a complementary folded cascode with 104.25 dB Voltage gain, 18.15 MHz of unity gain frequency and a slew rate of 13.370 MV/μs. These circuits were synthesized using a 0.35 μm technology. The results show that the method provides a fast approach for good solutions using the modified SA and further good Pareto front exploration through its connection to the particle swarm optimization algorithm.     

Technological development of Brazilian telecommunications in past decades

Brazilian telecommunications sector has undergone important modifications in the last 40 years. From a badly structured system of municipal level operators in 1960, with barely one million fixed lines to a large, nation-wide system with about 50 million fixed plus another 75 million mobile lines in 2005. This work paper reviews the institutional framework of telecommunications industrial and technological policy in Brazil in the last four decades, its results and present situation. Infrastructure and services modernisation and expansion process that happened in early 1970s were carried out at the expense of massive import of products and technology. A government strategy was, then, devised to stimulate local industrial and technological development in telecommunications, under the general guidelines of import substitution policy. Essential to this strategy was the creation of an innovation system around TELEBRÁS Research and Development Centre. This technological development model was successful while a protectionist economic policy existed. When economic and political circumstances could not anymore sustain an import substitution approach, in the 1990-decade, the model fell apart and has not been replaced by any other sectorial policy. A critical assessment is carried out, analysing the adopted actions in face of the digital technology maturing process that occurred in meantime.     

The influence of oxygen partial pressure on growth of the (Hg,Re)-1223 intergrain junction

Hg/sub 0.82/Re/sub 0.18/Ba/sub 2/Ca/sub 2/Cu/sub 3/O/sub 8+/spl delta// polycrystalline samples were successfully obtained by using different oxygen partial pressure in the annealing treatment of the precursor ceramic. The doping state was confirmed by X-ray powder diffraction pattern analysis and by observing distinct thermopower values at room temperature. Also, the intergrain regions have shown an improvement in the critical current density when using the precursor preparation with 10% O/sub 2/ and 90% Ar (optimal doped). The optimal doped sample has presented the highest /spl alpha/ exponent of the J/sub c//spl prop/[1-(T/T/sub c/)/sup 2/]/sup /spl alpha// dependence. For the case of (Hg,Re)-1223 polycrystalline superconductor applications, the /spl alpha/ exponent can be used as a junction quality parameter.