On the potential of combining neutron activation analysis and particle induced X-ray emission (PIXE) for micro- and microdistribution analysis of hard metals in human tissues

Tungsten carbide and cobalt are the main components of hard metal alloy while other metals such as chromium, niobium, tantalum, titanium and vanadium are sometimes added in smaller amounts. Exposure to hard metal dusts can induce a lung fibrosis with cobalt playing a major role. In order to provide information on the role that each metal may have in causing this disease, determination of the total content and the distribution of inhaled metals in lung tissue of hard metal workers is of paramount importance. However, samples such as transbronchial biopsy and bronchoalveolar lavage (BAL), often used in the medical diagnosis of pneumoconiosis, only allow for a small amount of material. This calls for sensitive and accurate analytical procedures for microdetermination and distribution of metals in pulmonary tissue and cellular material, such as macrophages. This work proposes a combination of sophisticated analytical techniques such as neutron activation analysis (NAA), currently applied to the determination of the total concentration of more than 30 elements in biological specimens, and PIXE analysis, particularly microPIXE, which has a great potential for microdistribution analysis in small biological samples. Principles and perspectives for the combined use of these techniques for the analysis of human tissue are outlined and discussed. NAA: determination of hard metals in lung tissue are carried out by neutron irradiation (2 × 10¹⁴ neutrons cm⁻² s⁻¹) in the HFR reactor of Petten. After neutron activation, radiochemical separations of ⁶⁰Co, ¹⁸⁷W, ¹⁸²Ta, ⁵¹Cr followed by computer-based high resolution gamma ray spectrometry allow the measurement of these elements in pulmonary tissues with sensitivities ranging from 10⁻⁴ μg (Cr) to 10⁻⁶ μg (W). PIXE: this technique is multielemental and of relatively high sensitivity (μg/g) even in small total sample masses of from 10 to 100 μg, thus allowing the analysis of parts of needle biopsies. Whereas PIXE has been successfully applied to many medical problems, its usefulness is limited in the total samples analysis for cobalt-related hard metal disease, due to the low levels of cobalt in tissue combined with severe element interferences from the generally more abundant metal, iron. Nevertheless, microPIXE, a special variety of the method scanning over the sample with a focussed ion beam of about 2 × 2 μm², could complement the NAA findings in total samples in the sense of achieving a microdistribution analysis of hard metals (including cobalt) in suitable thin tissue sections.The availability of specialized facilities at the JRC such as the powerful HFR reactor (Petten), the NAA laboratories (Ispra) and the microPIXE facility (Geel) could represent a European ‘reference pole’ for the study of metals in tissues of hard metal diseased subjects.     

Trace element analysis of Cretan wines and wine products

The object of this research is to investigate the ways and the degree of contamination of Cretan grapes from the area of Chania and their alcoholic products, with the elements aluminium, arsenic, cadmium, copper, chromium, iron, lead, manganese, nickel and zinc. Fifteen samples of grapes were collected and used for the production of experimental wines from rinsed and unrinsed grapes. A microwave furnace was used for the digestion and dissolution of the experimental wines, the precipitates that originated in these wines, as well as the wines of the corresponding producers. The analyses of all mentioned samples as well as 34 local alcoholic distillates were performed using total reflection X-ray fluorescence and graphite furnace atomic absorption spectrometry. The concentrations for all the elements that were determined were almost in all cases, well below the maximum permissible levels by the Greek and the European Union legislation.     

Soil salinity of urban turf areas irrigated with saline water: I. Spatial variability

With increasing use of saline water for irrigating urban landscapes, soil salinization is becoming a concern. This study examined spatial variation in soil salinity over the length of selected fairways at five golf courses, and of large turf areas at two public parks located in west Texas and southern New Mexico. Salinity of water used for irrigation ranged from 680 to 2700 mg L⁻¹, and the sites consisted mostly of Aridisols (upland soils) or Entisols (alluvial soils). Soil salinity distribution at sites consisting of deep Aridisols was spatially independent with the coefficient of variability (CV) ranging from 24 to 42%. The sites consisting of shallow Aridisols over a calcic horizon had erratic and random soil salinity distributions with an average CV of 37%. Since soil salinity distributions in Aridisols and Alfisols appeared to be spatially independent within the distance of fairways, soil sampling adequacy can be determined by the conventional probability statistics. However, salinity distributions at Entisols sites were spatially dependant to a length of 100 m or more. In addition, the clayey Entisols used for public parks had high levels of salt accumulation (>10 dS m⁻¹ in the saturation extract) with the CV exceeding 60%. When salinity readings were stratified by soil type distribution, the CV was reduced to 28% on the average. The number of samples required to obtain the mean salinity over a typical length of fairways (250–300 m) within a deviate range of 20% averaged 6 in deep sandy Aridisols and Alfisols, and 13 each in other cases, provided that sampling of Entisols is made based on soil type distributions. These sampling requirements are greater than those for field soil moisture or saturation water contents. The spacing to collect the required number of samples over the prevailing length of fairways was between 23 and 38 m. Soil sampling for salinity appraisal is most problematic in Entisols, but can be made simple if a detailed soil map is available. In Aridisols containing a calcic horizon, it is probable to have saline spots over poorly permeable caliche.     

A contribution to the study of some greek marbles

The use of marble in Architecture began on the 7th century B.C. and continued to be used without stop untill today. Greece and especially the East Macedonia region is an extremely privileged area on marble deposits both in quantities and qualities and colours. In the present work the main physical and engineering properties of some varieties of greek marbles are given and a comparison with the international standards is made. The experimental material (marbles) was from East Macedonia, Argolida-Arcadia and Magnessia, three of the biggest marble deposit areas in Greece. From the results obtained, it is concluded that the marbles examined satisfy all the international standard codes and thus they can be used as building stones and decoration materials.     

Post-crash fire testing of titanium fuselages

The problem … maintain fuselage integrity and a survivable cabin environment during a post-crash fire. Its high melting point made titanium an attractive candidate for the large-scale test discussed here. Note: This paper is an adaptation of Report No. FAA-RD-71-3, “Titanium Fuselage Environmental Conditions in Post-Crash Fires,” prepared by the author for the Federal Aviation Administration. The report was also the basis for Mr. Sarkos’ presentation at the 75th Annual Meeting of the National Fire Protection Association in San Francisco on May 18, 1971.     

Stress-Strain State of Weak Saturated Clay Beds of Embankments

Results are presented for laboratory and field tests of weak saturated soils serving as beds for embankments in Ho Chi Min City and various regions of the Mekong River Delta (Vietnam). A method is outlined for quantitative evaluation of the stress-strain state (SSS) of the weak saturated clayey beds (plane problem). A method is described for quantitative evaluation of the deformation and stability of weak beds in the initial, intermediate, and stabilized stages of the formation and transformation of the SSS. __________ Translated from Osnovaniya, Fundamenty i Mekhanika Gruntov, No. 5, pp. 2–6, September–October, 2005.     

Lead contamination in tap water of households with children in Lower Saxony, Germany

Lead has numerous acute and chronic adverse effects on human beings. This is especially true for infants and children. The main path of lead ingestion in children can be different according to housing and living situation. The intake of lead through drinking water is commonly due to metal corrosion. The users plumbing can be an important factor. In recent years, many lead pipes in Germany have been replaced by pipes made of an alternative material. The aim of this study is to assess the present state of drinking water contamination and the resulting exposure of infants to lead. For this purpose mothers of new-born babies were offered a free examination of their drinking water. After a written declaration of consent had been obtained and after the infant in question had reached an age of 3 months, a stagnation sample of cold tap-water after overnight stagnation together with a random daytime sample was obtained from the family. The collected samples were analysed by atomic absorption spectrometry for their lead concentration. In total, 1485 samples from households were collected. Of the 1434 stagnation samples, 3.1% had lead concentrations greater than 0.01 mg/l (recommended limit of the WHO) and 0.6% had concentrations above the limit of the German drinking water regulation (0.04 mg/l). The values for the 1474 random daytime samples were 2.1% above 0.01 mg/l and 0.2% greater than 0.04 mg/l, respectively. By region, the areas Bovenden, Friedland, Duderstadt, Northeim and Rosdorf were particularly affected. The highest measured concentrations of lead in the stagnation samples were 0.11 mg/l and 0.15 mg/l in the random daytime samples, respectively.     

Energy and environmental comparison of three variants of a family house during its whole life span

The aim of this study is to analyse and compare three variants of a family house in order to evaluate the total environmental impacts produced during the whole building life cycle. The first variant corresponds to the standard in force in Switzerland, the second alternative to the requirements of a quality control label for houses with low energy consumption and the third case is a very low energy consumption building. The three variants have the same architectural aspect but different insulation thicknesses and types, different energy production systems and the use of different renewable energies. The calculation of the environmental impacts is carried out by means of a life cycle analysis, which includes not only the impact related to the energy consumption during the occupancy stage, but also the materials manufacture, transport, replacement and elimination at the end of the building lifetime. The results are expressed with the Swiss and the UCTE (Union for the Co-ordination of Transmission of Electricity) electricity mix to analyse how they influence the building impact.