Limonoid Debittering of Citrus Juice Sera By Immobilized Cells of Corynebacterium fascians

Corynebacterium fascians cells capable of metabolizing limonoids were prepared conveniently with inexpensive carbon sources such as fructose, galactose and citric acid. Cells thus obtained were immo-bilized in acrylamide gel and used in a biological debittering process previously developed. The process significantly reduced limonin and nomilin contents of citrus juice sera. It was particularly effective toward the reduction of nomilin. The debittering treatment did not have adverse effects on the composition of other citrus constituents such as citric, malic, ascorbic acids, fructose, glucose and sucrose.     

Neo-liberal or Third Way? What Planners from Glasgow,Melbourne and Toronto Say

Given the long and well-documented decline in the existence and relevance of place-dependent urban community, we explore the use of an idealised version of community of place in the marketing and implementation of a new suburban master planned estate (MPE) in Australia. In a social policy environment where strong communities of place are idealised, we find residents lack the resources or inclination to devote to the establishment of civic norms or durable social networks within the boundaries of the development and, for the most part, they are content to leave the establishment of the symbols of community to the property developer. It is the idea, rather than the reality, of community that is important, but in order to generate that idea, the developer needs to go to some lengths to create an ‘illusion’, or ‘sense’ of strong local community in the estate through marketing, the creation of nostalgic physical reminders, and selected community interventions such as cyclical events and celebrations. These all play an important role in the establishment of subjective ontological security for residents; to the benefit of both resident and developer.

     

Outcomes from Community Engagement in Urban Regeneration: Evidence from England's New Deal for Communities Programme

This article assesses the potential for non-statutory planning tools, Local Landscape Designations (LLDs), to deliver rural development in Scotland. This research is motivated by new Scottish guidance which encourages designations in general, and LLDs in particular, to be more positive, strategic tools for rural development activities. LLDs were originally conceived to protect and enhance significant areas of valued countryside yet they remain neglected in contemporary planning research and policy. A critical assessment of LLDs across Scotland was undertaken using both primary and secondary data. The results reveal a significant mismatch between positive national guidance and local authority policy and planner attitudes, the latter characterised by more protectionist positions and limited strategic planning. Although case study findings in Aberdeenshire reveal that more planning applications in LLDs have conditional approvals as opposed to the wider countryside, key differences have more to do with the subjective views and priorities of planning committees rather than the efficacy of LLDs per se. The article concludes that the current conceptualisation, operation and performance of LLDs needs to be more robust, and better aligned to reflect a positive focus on community and rural development matters in order to differentiate them from wider countryside policies. However, augmenting resources to reflect the increased attention to the landscape agenda in Scotland is a core prerequisite.     

Accessing osteocyte lacunar geometrical properties in human jaw bone on the submicron length scale using synchrotron radiation μCT

The architectural properties of the osteocyte cell network provide a valuable basis for understanding the mechanisms of bone remodelling, mineral homeostasis, ageing and pathologies. Recent advances in synchrotron microtomography enable unprecedented three‐dimensional imaging of both the bone lacunar network and the extracellular matrix. Here, we investigate the three‐dimensional morphological properties of osteocyte lacunae in human healthy and bisphosphonate‐related osteonecrotic jaw bone based on synchrotron X‐ray computed tomography images, with a spatial isotropic voxel size of 300 nm. Bisphosphonate‐related osteonecrosis of the jaw is a relatively new disease with increasing incidence, which remains poorly understood. A step forward in elucidating this malady is to assess whether, and how, the morphology of the osteocyte lacunar network is modified in the affected jaw tissue. We evaluate thousands of cell lacunae from five specimens of which three originate from patients diagnosed with bisphosphonate‐associated osteonecrosis. In this exploratory study, we report three‐dimensional quantitative results on lacunar volumes (296–502 μm³), shape (approximated by an ellipsoidal shape with principal axes a > b > c, such that a = 2.2b and a = 4c) and spatial distribution (i.e., 50% of the mineralized matrix volume is located within 12 μm to the closest lacunar boundary) at submicron resolution on such specimens. We observe that the average lacunar volumes of the bisphosphonate‐related osteonecrotic jaw specimens were within the range of volumes found in the two specimens originating from healthy donors and conclude that lacunar volumes are not the key element in the course of bisphosphonate‐related osteonecrotic jaw. In three out of five specimens we observe lacunar volume sizes in segmented osteons to be significantly different compared to lacunar volumes in the adjacent tissue regions. Furthermore, we quantify the number of lacunae containing small dense objects (on average 9%). In contrast to lacunar morphology we report the lacunar density (16 000–50 000 per mm³) to be different in jaw bone tissue compared to what has been reported in femoral sites.     

WHEY SEPARATION IN ACID SKIM MILK GELS MADE WITH GLUCONO-δ-LACTONE: EFFECTS OF HEAT TREATMENT AND GELATION TEMPERATURE

The effects of heat treatment and gelation temperature on whey separation in acid milk gels made with glucono-δ-lactone were studied using three empirical methods, two of which were developed specifically to quantify spontaneous whey separation from set acid gels. Gels were made in volumetric flasks and petri dishes and the amount of surface whey produced by gels after 16 h was compared with the amount of supernatant expressed by low speed centrifugation (100 g X 10 min) of gels made in tubes. A central composite experiment design and response surface methodology were used. Using regression analysis a second order polynomial model satisfactorily predicted the effect of heat treatment and gelation temperature on whey separation for gels made in volumetric flasks (R²= 0.89). Whey separation was significantly increased by heat treatment (P < 0.001), gelation temperature (P < 0.01) and the quadratic term for heat treatment (P < 0.01). A significant (P < 0.01) positive correlation (r = 0.71) was observed between whey separation in volumetric flasks and petri dishes. It was suggested that high heat treatments and gelation temperatures favour more rearrangements of the network just after formation, making gels unstable and sensitive to spontaneous whey separation.     

Palatability and Storage Characteristics of Precooked Beef Roasts

The palatability and storage characteristics of fresh, frozen and precooked beef chuck roasts prepared using added phosphate-salt and vacuum cooking bags were evaluated. Palatability attributes (tenderness, juiciness, beef flavor intensity, and off-flavor intensity) of precooked roasts prepared in vacuum cooking bags with phosphate-salt were as good or better than freshly cooked roasts. Precooked roasts in vacuum cooking bags had the lowest total bacterial counts after storage for 14 and 28 days. A palatable precooked roast beef product was developed that can be stored for 28 days at 4°C.     

Emulsion Stability as Affected by Competitive Adsorption Between an Oil-Soluble Emulsifier and Milk Proteins at the Interface

The effect of an oil-soluble emulsifier upon adsorption properties of β-casein and β-lactoglobulin at the oil-water interface was studied. Addition of emulsifier improved the emulsification properties, but lowered emulsion stability. Higher concentrations decreased emulsification capacity, due to an increase in oil phase viscosity, and interfered with several protein assays, except the o-phthaldialdehyde (OPA) assay. Depletion measurements revealed that the emulsifier displaced β-casein more readily than β-lactoglobulin from the surface of droplets, which correlated with emulsification and stability measurements. Displacement of proteins was not complete due to emulsifier-induced formation of a multiple w-o-w emulsion, which accounted for the missing protein in the continuous phase.     

跃变比判别热解Si-C-N陶瓷EELS定量分析的有效性

电子能量损失谱（EELS）分析方法的运用为材料研究领域提供了有力的定性和定量手段。通过对内壳层电子激发谱跃变比的计算,能够获得样品相对厚度的参考信息。这一方法弥补了实验条件在同步采集等离子激发谱和内壳层电子激发谱方面的局限性,并为辨别EELS定量的可靠性提供依据。本文以Si3N4为例,对跃变比随样品相对厚度的变化规律进行了研究,并在低于最佳样品厚度的范围内得到了可靠的定量结果。类似的跃变比变化规律能够运用于成份均一的Si-C-N热解样品,然而对于非均一的Si-C-N退火样品则不适用。     

Joint lot-sizing and scheduling for multi-stage multi-product flow shops

Here we consider a multi-product flow shop under capacity constraints and develop an efficient heuristic procedure to determine joint lot sizes, production sequence and production schedule. We treat setup costs as fixed in the short run and thus independent of the number of setups. Loss of production capacity due to setup times is explicitly accounted for and the transfer of portions of a production lot between stages is permitted. The procedure is based on identifying the bottleneck workcentre and synchronizing production schedules at all other workcentres with the bottleneck workcentre such that product throughput requirements are met with minimal inventory costs. A compact procedure for constructing the Gantt charts is also presented. Conversion of the lot si2es and transfer batches into an information control system with kanbans is also presented.     

Effects of Postmortem Storage and Temperature on Muscle Protein Degradation: Analysis by SDS Gel Electrophoresis

The proteolytic breakdown of the major contractile proteins of bovine longissimus muscle was examined during postmortem storage by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. Samples of muscle stored at 4°C for 14 days exhibited little proteolysis of the major contractile proteins; however, samples stored at 37°C for 1 day showed significant degradation of myosin heavy chains and almost complete proteolysis of this protein by day 14. Major degradation products of the myosin heavy chains included a series of polypeptides having molecular weights between 145,000 and 125,000. These experiments demonstrate that substantial degradation of the myosin heavy chain and other muscle proteins can occur during the storage of meat, and this phenomenon was highly temperature dependent.