Teichoic acid content in the cell wall of Actinomyces rimosus LST-118 in the process of ontogeny

Polymeric compounds of the cell walls of Act. rimosus were studied during the actinomycete growth. It was found that the content of teichoic acid decreased 2 times by the end of development of Act. rimosus, while content of glycane polymer remained approximately at the same level. The molar ratios of teichoic acid and glycopeptide glycane were determined.     

Modeling the atomic diffusion coefficient of channeled particles

The atomic diffusion coefficient of channeled particles has been constructed within the Doyle–Turner approximation of the potential of an isolated atom. It is shown that the atomic diffusion coefficient seeks its minimum value in the region of the maximum nuclear density of the atomic chain where the Kitagawa–Ohtsuki diffusion coefficient reaches its maximum value. Atomic diffusion coefficients have been modeled with the TROPICS software that was upgraded for calculations on heterogeneous structures.     

Discovery of the improved antagonistic prolactin variants by library screening

Prolactin (PRL), a potent growth stimulator of the mammary epithelium, has been suggested to be a factor contributing to the development and progression of breast and prostate cancer. Several PRL receptor (PRLR) antagonists have been identified in the past decades, but their in vivo growth inhibitory potency was restricted by low receptor affinity, rendering them pharmacologically unattractive for clinical treatment. Thus, higher receptor affinity is essential for the development of improved PRLR antagonistic variants with improved in vivo potency. In this study, we generated Site 1 focused protein libraries of human G129R-PRL mutants and screened for those with increased affinity to the human PRLR. By combining the mutations with enhanced affinities for PRLR, we identified a novel G129R-PRL variant with mutations at Site 1 that render nearly 50-fold increase in the antagonistic potency in vitro.     

Hemosorption in the prevention of severe forms of hemolytic disease of the newborn in rhesus sensitization

A total of 164 hemoperfusions were administered to 60 pregnant patients with Rh sensitization and a history of perinatal death of the newborns from hemolytic disease. Hemoperfusion was found to decelerate the progress of hemolytic disease of the newborns promoting delivery of babies with more benign forms of the disease. The method efficacy was 76%. Dynamic follow-up of fetal functional status over the course of hemosorption treatment by examinations of biophysical profiles and ultrasonic dopplerometry has shown no negative effects of this treatment modality on fetal status.     

Calculation of Structural Characteristics Evolution during Superplastic Forming of Shells by

The applications of commercial computer program set "SPLEN-O" based on finite elements method (FEM) for the simulation of superplastic forming (SPF) technology of shells are described. The changes in characteristics of ultrafine-grained (UFG) structure during deformation are taken into account in function of viscosity and solving algorithm. Some examples are shown to illustrate the possibilities of FEM simulation for prediction of numerical grain-size data on different SPF stages of shells.     

Expression Profile of miRs in Mesial Temporal Lobe Epilepsy: Systematic Review

Temporal lobe epilepsy (TLE) is one of the most common forms of focal epilepsy in children and adults. TLE is characterized by variable onset and seizures. Moreover, this form of epilepsy is often resistant to pharmacotherapy. The search for new mechanisms for the development of TLE may provide us with a key to the development of new diagnostic methods and a personalized approach to the treatment. In recent years, the role of non-coding ribonucleic acids (RNA) has been actively studied, among which microRNA (miR) is of the greatest interest. (1) Background: The purpose of the systematic review is to analyze the studies carried out on the role of miRs in the development of mesial TLE (mTLE) and update the existing knowledge about the biomarkers of this disease. (2) Methods: The search for publications was carried out in the databases PubMed, Springer, Web of Science, Clinicalkeys, Scopus, OxfordPress, Cochrane. The search was carried out using keywords and combinations. We analyzed publications for 2016–2021, including original studies in an animal model of TLE and with the participation of patients with TLE, thematic and systemic reviews, and Cochrane reviews. (3) Results: this thematic review showed that miR‒155, miR‒153, miR‒361‒5p, miR‒4668‒5p, miR‒8071, miR‒197‒5p, miR‒145, miR‒181, miR‒199a, miR‒1183, miR‒129‒2‒3p, miR‒143‒3p (upregulation), miR–134, miR‒0067835, and miR‒153 (downregulation) can be considered as biomarkers of mTLE. However, the roles of miR‒146a, miR‒142, miR‒106b, and miR‒223 are questionable and need further study. (4) Conclusion: In the future, it will be possible to consider previously studied miRs, which have high specificity and sensitivity in mTLE, as prognostic biomarkers (predictors) of the risk of developing this disease in patients with potentially epileptogenic structural damage to the mesial regions of the temporal lobe of the brain (congenital disorders of the neuronal migration and neurogenesis, brain injury, neuro-inflammation, tumor, impaired blood supply, neurodegeneration, etc.).     

Polymorph prediction of organic pigments

The crystal structure of several industrially important organic pigments namely C.I. Pigment Violet 23, C.I. Pigment Red 202 and C.I. Pigment Yellow 139 were predicted using the Cerius² Polymorph Predictor in combination with XRPD patterns. After generation and energy minimisation of the candidate structures for each pigment, their calculated powder patterns were compared with those obtained experimentally using X-ray diffraction. The structures which best fitted the experimental powder patterns were regarded as possible structures of the compound; finally, a rigid body Rietveld refinement was performed to validate the choice of the structure. The structure of C.I. Pigment Violet 23 has not previously been published while the predicted structures of C.I. Pigment Red 202 and C.I. Pigment Yellow 139 were in accordance with the published structures. The work demonstrates that pigment crystal structure can be predicted using a low quality X-ray powder diffraction pattern.     

Magneto-impedance element

The magneto-impedance (MI) effect is a phenomenon in which the voltage induced by a high frequency current source in a ferromagnetic wire changes with the application of an external field. A giant MI effect was found in amorphous magnetic wires having a composition of (Fe _0.06Co_0.94)_72.5Si_12.5B ₁₅ and a magnetostriction of (-10^-7). The amplitude of the wire voltage decreased by 40% at 1 MHz, 60% (600 kHz) and 50% (150 kHz), for wires having diameters 30 μm, 50 μm and 124 μm, respectively, under the influence of an external longitudinal field of about 10 Oe (800 A/m). A highly sensitive and quick-response field sensor was constructed using a 200 MHz resonant multivibrator bridge-circuit combining two MI-effect elements of 1 mm length with two field effect transistors (FET). Highly sensitive flux detection was carried out by using the small MI sensor head on a rotary encoder magnet having 512 poles and a diameter of 30 mm. Discussion of a mechanism for the MI effect considers the skin effect in an amorphous wire with high circumferential anisotropy