Improvement of polyvinyl alcohol/casein blend film properties by adding cellulose nanocrystals

This study aims to prepare and examine the properties of poly(vinyl alcohol)/casein (PVA/CAS) based films reinforced with cellulose nanocrystals (NC), which can be presented as an alternative to petroleum-based polymer packaging materials. PVA/CAS and 0.5–1–3–5 wt% NC containing PVA/CAS biocomposite films were prepared by solution casting method. Afterward, the 1NC film, which exhibited the best mechanical properties, was crosslinked with various amounts of glyoxal. Structural, morphological (polarized optical microscope), mechanical (tensile), thermal (differential scanning calorimetry, thermogravimetric analysis), contact angle, and water vapor transmission rate (WVTR) properties of the samples were investigated. The 1NC film exhibited the highest tensile strength (TS) and elongation values in PVA/CAS/NC films, and its mechanical properties decreased due to agglomeration with increasing NC amount. As expected, crosslinking improved the TS. The thermal stability of the PVA/CAS film was generally improved with the addition of NC and crosslinking. The high WVTR value of the PVA/CAS film decreased with the addition of NC and the 1NC film presented the lowest value. Thanks to the complex structure formed as a result of crosslinking and the reduced free volume, the WVTR of the 1NC film has reduced. The results showed that PVA/CAS-based films with good mechanical properties and water vapor barrier are promising as packaging materials.     

Correlative Light-Ion Microscopy for biological applications

Here we report a new technique, Correlative Light-Ion Microscopy (CLIM), to correlate SEM-like micrographs with fluorescence images. This technique presents significant advantages over conventional methods in enabling topographical and biochemical information to be correlated with nanoscale resolution without destroying the fluorescence signal. We demonstrate the utility of CLIM for a variety of investigations of cell substrate interactions validating its potential to become a routine procedure in biomedical research.     

A fast minimization technique for subspace fitting with arbitraryarray manifolds

A new technique for minimization of a multidimensional subspace fitting criterion is proposed. An approximation is made to the exact criterion function, giving an explicit least squares solution for location parameters. Ideas from alternating projections are employed for initialization. The new technique gives global convergence with a high probability, has a low computational cost, is applicable to general array manifolds, and provides simple solutions for many problems with intricate parameterization     

Damage Identification on the Tilff Bridge by Vibration Monitoring Using Optical Fiber Strain Sensors

Vibration testing is a well-known practice for damage identification of civil engineering structures. The real modal parameters of a structure can be determined from the data obtained by tests using system identification methods. By comparing these measured modal parameters with the modal parameters of a numerical model of the same structure in undamaged condition, damage detection, localization, and quantification is possible. This paper presents a real-life application of this technique to assess the structural health of the 50-year old bridge of Tilff, a prestressed three-cell box-girder concrete bridge with variable height. A complete ambient vibration survey comprising both vertical accelerations and axial strains has been carried out. The in situ use of optical fiber strain sensors for the direct measurement of modal strains is an original contribution of this work. It is a big step forward in the exploration of modal curvatures for damage identification because the accuracy in calculating the modal curvatures is substantially improved by directly measuring modal strains rather than deriving the modal curvatures from acceleration measurements. From the ambient vibrations, natural frequencies, damping factors, modal displacements and modal curvatures are extracted by the stochastic subspace identification method. These modal parameters are used for damage identification which is performed by the updating of a finite element model of the intact structure. The obtained results are then compared to the inspections performed on the bridge.     

Occurrence of aflatoxins (B1, B2, G1, G2) in herbal tea consumed in Turkey

Aflatoxin contents in 12 types of herbal teas were determined by high performance liquid chromatography (HPLC) with fluorescence detector using immunoaffinity column clean-up. Forty eight samples were collected from four local herbal shops in Manisa, Turkey. Of the 48 samples analyzed, 43 were aflatoxin positive. The highest concentration of aflatoxin (34.18 µg/kg) was determined in a sample of camomile tea. The occurrence of AFB1, B2, G1 and G2 was found in samples at levels of 54, 29, 71 and 46 %, respectively. Aflatoxin B1, B2, G1 and G2 contamination levels varied from 0 to 14.2, 0 to 12.4, 0 to 13.5 and 0 to 28.7 µg/kg, respectively. Aflatoxin was not detected in five samples consisting of linseed, lime and fennel tea.     

Links between Painting and Photography in Nineteenth-Century Turkey

It is well known that engravings were used by artists as models for their paintings. Another visual source employed by painters was the photograph. This occurred in Turkey after photography was introduced into Ottoman territories as a result of visits made by Europeans to the Ottoman states. In addition, courses on photography were added to the curricula of the schools. Although photography was initially received negatively by the public, the positive attitude of the Ottoman Sultans determined the popularity and acceptance of this branch of art. The introduction of photography and its adoption by the Istanbul public occurred first during the reign of Sultan Abdülmecid (1839–1861). Sultans Abdülaziz (1861–1876) and Abdulhamid II (1876–1909) played significant roles in the development of Ottoman photography. In addition, Abdulhamid II appointed photographers to document the events, institutions and structures in the empire and had around 800 photograph albums prepared. In these albums important settlements, structures and gardens of the period are documented. In wall paintings and in paintings on canvas, photographs were notably used as sources.     

A Two‐Sided Cumulative Sum Chart for First‐Order Integer‐Valued Autoregressive Processes of Poisson Counts

Count data processes are often encountered in manufacturing and service industries. To describe the autocorrelation structure of such processes, a Poisson integer‐valued autoregressive model of order 1, namely, Poisson INAR(1) model, might be used. In this study, we propose a two‐sided cumulative sum control chart for monitoring Poisson INAR(1) processes with the aim of detecting changes in the process mean in both positive and negative directions. A trivariate Markov chain approach is developed for exact evaluation of the ARL performance of the chart in addition to a computationally efficient approximation based on bivariate Markov chains. The design of the chart for an ARL‐unbiased performance and the analyses of the out‐of‐control performances are discussed. Copyright © 2012 John Wiley & Sons, Ltd.     

Species distribution,molecular characteristics and vancomycin resistance gene profiles of Enterococcus sp. isolates from farmhouse cheeses in western Turkey

Species distribution, virulence traits and vancomycin resistance gene profiles of Enterococcus isolated from 43 home‐made artisan cheese samples collected from open markets, located in Aydin region of Turkey, were investigated. Of the 129 isolates, 95 were identified as Enterococcus sp.; Enterococcus faecium being the most prevalent species (82.1%), followed by Enterococcus faecalis (13.6%) and Enterococcus durans (1.0%). None of the enterococci were harbouring vanA or vanC, while seven isolates (7.3%) were shown to harbour vanB gene by multiplex PCR. gelE (49.4%) being the most prevalent virulence factor was followed by asa1 (27.3%), esp (22.1%), cylA (4.2%) and hyl (3.1%).     

Optimization of particle number,substrate concentration and temperature of batch immobilized reactor system for biohydrogen production by dark fermentation

Immobilized cell bioreactor was operated in batch mode for biohydrogen generation by dark fermentation from acid hydrolyzed waste wheat powder. It was aimed to optimize the fermentation conditions with the purpose of obtaining the highest hydrogen yield (Y_H2) and production rate (HPR) by applying Box–Wilson statistical experimental design method. Particle number (PN = 120–240; X₁), initial total sugar concentration (TS₀ = 10–30 g/l; X₂) and fermentation temperature (T = 35–55 °C; X₃) were selected as independent variables. Polyester fibers with particle diameter “Dp” = 0.5 cm were used as support material to immobilize microorganisms with heat-pretreated sludge. Quadratic equations for production yield and rate were developed by using experimental results. The maximum Y_H2 (3.21 mol H₂/mol glucose) and HPR (73.3 ml H₂/h) were predicted at the optimum conditions of PN = 240, TS₀ = 10 g/l and T = 44.9 °C. Also, analysis of variance, as well as sum of ranking difference test results demonstrated that fitting models were statistically significant.