Selective isolation of reversible cold sensitive variants from Chinese hamster ovary cell cultures

The fine structure and cellular associations of the large pigment cells (LPC's) of the compound eye of the house fly were studied with high voltage and conventional electron microscopy. Depending on the sector of the compound eye, the facets are either rectangular or hexagonal. The underside of each facet has indentations exactly aligned with those on top into which inserts an angulated sleeve of LPC's. Under the rectangular lens facet 6 or 8 small compact (in cross section) LPC's join four elongate LPC's. Clusters of compact cells alternate in this ring with elongate ones. Compact cells compress together and become quadrangular (in cross section) several microns below their insertion into the lens and form "building block" corners while elongate cells form "side rails" for the rectangular type of distal pseudocone enclosure. Beneath hexagonal facets all LPC's are rather elongate with out corner cells. In both facet types LPC's enclose the pseudocone for a longitudinal distance of 4 mum and then are displaced as bordering cells by a sleeve of two corneal pigment cells (CPC's), each of which encloses half of the proximal pseudocone. For the following 6 mum of longitudinal distance these concentric sleeves of CPC's and LPC's form a double layer around the pseudocone. At about 10 mum below lens base the two sleeves separate; LPC's become attenuated and extend cable-like to the basement membrane and CPC's enclose the proximal pseudocone, Semper cells and distal retinula. The junction between lens and LPC's has critical structural value in that (1) this is the sole anchorage to the lens by the lengthy remainder of the ommatidium, and (2) LPC's enclose the semiliquid pseudocone in the most distal portion of the pseudocone. In addition to vertical support, the LPC's send out numerous lateral processes that make structural contact among themselves, with the corneal pigment cells and the photoreceptor cells. The structural features of this array are discussed relative to possible physiological roles.     

Distributed Job Scheduling in Rings

We give a distributed approximation algorithm for job scheduling in a ring architecture. In contrast to many other parallel scheduling models, the model we consider captures the influence of the underlying communications network by specifying that task migration from one processor to another takes time proportional to the distance between those two processors in the network. As a result, our algorithm must balance computational load and communication time. The algorithm is simple, requires no global control, and yields schedules of length at most 4.22 times optimal. We also give a lower bound on the performance of any distributed algorithm and the results of simulation experiments which suggest better performance than does our worst-case analysis.     

A Mouse-Based Method to Monitor Wheat Allergens in Novel Wheat Lines and Varieties Created by Crossbreeding: Proof-of-Concept Using Durum and A. tauschii Wheats

Wheat allergies are potentially life-threatening because of the high risk of anaphylaxis. Wheats belong to four genotypes represented in thousands of lines and varieties. Monitoring changes to wheat allergens is critical to prevent inadvertent ntroduction of hyper-allergenic varieties via breeding. However, validated methods for this purpose are unavailable at present. As a proof-of-concept study, we tested the hypothesis that salt-soluble wheat allergens in our mouse model will be identical to those reported for humans. Groups of Balb/cJ mice were rendered allergic to durum wheat salt-soluble protein extract (SSPE). Using blood from allergic mice, a mini hyper-IgE plasma bank was created and used in optimizing an IgE Western blotting (IEWB) to identify IgE binding allergens. The LC-MS/MS was used to sequence the allergenic bands. An ancient Aegilops tauschii wheat was grown in our greenhouse and extracted SSPE. Using the optimized IEWB method followed by sequencing, the cross-reacting allergens in A. tauschii wheat were identified. Database analysis showed all but 2 of the durum wheat allergens and all A. tauschii wheat allergens identified in this model had been reported as human allergens. Thus, this model may be used to identify and monitor potential changes to salt-soluble wheat allergens caused by breeding.     

A Sharp Interface Model of Intermediate-Phase Growth Under the Influence of Electromigration

A sharp interface model has been developed to model intermediate-phase growth under the influence of electromigration in a binary system. Simulation results show that the phase growth rate depends on both the magnitude and the direction of the applied current. With the current density fixed, at early times, there is a parabolic growth behavior for the intermediate phase, while at longer times, there is a linear phase growth behavior when the electron flow aids diffusion of atoms; however, a limiting thickness is found when the electron flow hinders diffusion. Qualitative analysis shows that the longer time behavior also holds in a binary system with multiple intermediate phases present.     

Extending and operationalizing construction productivity measurement on building projects

Measuring construction productivity in an accurate, repeatable and reliable way represents a significant problem at a building project level of enquiry. The research develops a measurement framework that balances simplified data gathering against explanatory ability. It aims to assist analytically driven process improvement. Debate and discourse are used to draw upon the extant literature in creating a logic-based flow of ideas to develop the framework. Targeted use of empirical cost and site process data from case studies are presented to punctuate the discourse. The derived approach advocates multifactor measurement inclusive of labour and temporary production infrastructure inputs, set against Gross Floor Area outputs. Emphasis is placed on separately measuring homogenous and heterogeneous parts of work processes. A reductionist approach is used to operationalize measurement where projects are categorized according to fitness-for-purpose criteria and links made between systems, activities and tasks. Emphasizing measurement of homogenous parts of the work is new to the literature. It enables delineated measurement between work involving mass economies of scale (homogenous work), versus customized work (heterogeneous work). The approach enables improved benchmarking, diagnostic and predicative options. A course of ongoing research is provided to assist ongoing development and implementation.     

Sustainable construction: life cycle energy analysis of construction on sloping sites for residential buildings

In 2010, the Australian residential construction sector contributed about 28% of the value of all construction and was responsible for 8% of the total energy consumption. Residential construction will continue to increase to cope with the demand due to population growth. Owing to land scarcity, construction on sloping sites has become a common construction method for residential development in Australia. This method has economic benefits but poses environmental issues as it damages topsoil, disturbs natural drainage and groundwater pathways and imposes additional stress on soil under fill. The life cycle energy consumption of the construction process is examined in relation to residential projects on sloping sites on a range of slopes and soil types in New South Wales, Australia. Forty-one detached dwellings were selected and a service life of 60 years assumed for the study. The research findings reveal that the slope for each type of soil has a positive correlation with life cycle energy consumption. As part of the onsite construction process, the results also show that the energy consumption of construction on sloping sites plays a significant factor in the life cycle energy analysis of a building.     

Guest editorial–special issue on ground control in mining in 2020

正Ground control is the science of studying and controlling the behavior of rock strata in response to mining operations.Ground-control-related research has seen significant advancements over the last 40 years, and these accomplishments are well documented in the proceedings of the annual International Conference on Ground Control in Mining (ICGCM)[1].     

The distribution, abundance and diversity of birds in Manila's last greenspaces

Urban environments are not considered areas with conservation importance. However greenspaces in cities have been previously identified as areas with significant avian biodiversity. We investigated the distribution and diversity of birds in what are increasingly considered as Metropolitan Manila's last greenspaces; the University of the Philippines campus, military cemeteries and two government operated parks. Using species–area analysis, abundance and diversity indices, TWINSPAN ordination and logistic regression to determine important landscape features for species presence, we describe the distribution of bird communities and diversity in Metropolitan Manila. Two major bird community groupings were observed. These are the urban exploiters and the urban adaptable with the former occurring in high abundances and the latter in low abundances in greenspaces. The number of built and natural spatial entities determines abundances. Species area analysis suggests that the greenspaces are distinct habitats that preserve faunal uniqueness whereas urbanization tends to decrease diversity. These observations suggest that greenspaces harbor significant avian biodiversity as well as the presence of endemic and threatened species. As the greenspaces possess remnant wetland and wooded habitat we recommend that they be preserved and maintained by ensuring that these habitats are incorporated in any urban development plan.