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SA Gould EE Moore FA Moore JB Haenel JM Burch H Sehgal L Sehgal R DeWoskin GS Moss 《Canadian Metallurgical Quarterly》1997,43(2):325-31; discussion 331-2
We have previously documented the safety of 1 unit (50 gram) of human polymerized hemoglobin (Poly SFH-P) in healthy volunteers. This report describes the first patient trial to assess the therapeutic benefit of Poly SFH-P in acute blood loss. Thirty-nine patients received 1 (n = 14), 2 (n = 2), 3 (n = 15), or 6 (n = 8) units of Poly SFH-P instead of red cells as part of their blood replacement after trauma and urgent surgery. There were no safety issues related to the infusion of Poly SFH-P. The plasma hemoglobin concentration ([Hb]) after the infusion of 6 units (300 gram) of Poly SFH-P was 4.8 +/- 0.8 g/dL (mean +/- SD). Although the red cell [Hb] fell to 2.9 +/- 1.2 g/dL, the total [Hb] was maintained at 7.5 +/- 1.2 g/dL. Poly SFH-P maintained total [Hb], despite the marked fall in red cell [Hb] due to blood loss. The utilization of O2 (extraction ratio) was 27 +/- 16% from the red cells and 37 +/- 13% from the Poly SFH-P. Twenty-three patients (59%) avoided allogeneic transfusions during the first 24 hours after blood loss. Poly SFH-P effectively loads and unloads O2 and maintains total hemoglobin in lieu of red cells after acute blood loss, thereby reducing allogeneic transfusions. Poly SFH-P seems to be a clinically useful blood substitute. 相似文献
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SJ Gould 《Canadian Metallurgical Quarterly》1998,353(1366):307-314
For principled and substantially philosophical reasons, based largely on his reform of natural history by inverting the Paleyan notion of overarching and purposeful beneficence in the construction of organisms, Darwin built his theory of selection at the single causal level of individual bodies engaged in unconscious (and metaphorical) struggle for their own reproductive success. But the central logic of the theory allows selection to work effectively on entities at several levels of a genealogical hierarchy, provided that they embody a set of requisite features for defining evolutionary individuality. Genes, cell lineages, demes, species, and clades-as well as Darwin's favoured organisms-embody these requisite features in enough cases to form important levels of selection in the history of life. R. A. Fisher explicitly recognized the unassailable logic of species selection, but denied that thsi real process could be important in evolution because, compared with the production of new organisms within a species, the origin of new species is so rare, and the number of species within most clades so low. I review this and other classical arguments against higher-level selection, and conclude (in the first part of this paper) that they are invalid in practice for interdemic selection, and false in principle for species selection. Punctuated equilibrium defines the individuality of species and refutes Fisher's classical argument based on cycle time. In the second part of the paper, I argue that we have failed to appreciate the range and power of selection at levels above and below the organismic because we falsely extrapolate the defining properties of organisms to these other levels (which are characterized by quite different distinctive features), and then regard the other levels as impotent because their effective individuals differ so much from organisms. We would better appreciate the power and generality of hierarchical models of selection if we grasped two key principles: first, that levels can interact in all modes (positively, negatively, and orthogonally), and not only in the negative style (with a higher level suppressing an opposing force of selection from the lower level) that, for heuristic and operational reasons, has received almost exclusive attention in the existing literature; and second, that each hierarchical level differs from all others in substantial and interesting ways, both in the style and frequency of patterns in change and causal modes. 相似文献
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Previously we showed that cGMP hydrolysis in rat whole retinal homogenates exhibited a dose-dependent inhibition following developmental lead exposure and a concentration-dependent inhibition with direct Pb2+ exposure. Additionally, developmental lead exposure resulted in a dose-dependent increase in retinal cGMP and rod Ca2+ levels. To determine whether Pb2+ or Ca2+ directly inhibited the rod-specific cGMP phosphodiesterase (PDE) and to examine the kinetic mechanism of this inhibition, purified bovine rod cGMP PDE was assayed in the presence of varying concentrations of cGMP, and Mg2+, Pb2+, and/or Ca2+. Increasing concentrations of the substrate, cGMP, resulted in a shift of the Pb2+ and Ca2+ concentration-response curves to the left, indicating a decrease in the half-maximal inhibitory concentrations of Pb2+ from nanomolar to picomolar levels. Increasing concentrations of the cofactor, Mg2+, resulted in a shift of the Pb2+ and Ca2+ concentration-response curves to the right, indicating a decrease in the inhibition of PDE activity by Pb2+ or Ca2+. A plot of 1/velocity vs 1/Mg2+ as a function of Pb2+ revealed that picomolar concentrations of Pb2+ competitively inhibited PDE relative to millimolar concentrations of Mg2+. Consistent with this finding, Mg2+ reversed the Pb(2+)-induced inhibition of PDE. Our recent kinetic analysis showed that Mg2+ and cGMP bind at interacting sites on the PDE in a random order. The present results reveal that Pb2+ may bind at the same site but with 4-6 log units higher affinity than Mg2+, thus preventing the hydrolysis of cGMP. These findings provide a novel mechanism for understanding the Pb(2+)-induced inhibition of cGMP PDE. These results may have implications for other enzymes using Mg2+ as a cofactor and suggest that Mg2+ may be useful in these situations for reversing the inhibition by Pb2+. 相似文献
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In an empirical case study of software tools, two participants used the ITS style designer's language to implement a general purpose, executable, rule-based user interface style. This language allows style designers to select, combine and modify rule prototypes in order to construct a rule-based user interface style. The participants implemented the entire IBM CUA-2 user interface style, plus nine additional human-computer interaction techniques, in 5–7 person-weeks. This is impressive productivity. Typically the time to complete a single CUA-2 application is measured in person-years, not person-weeks. The style implemented here is reusable by any ITS-implemented application. The achievement reported here shows that about half the work of all ITS-implemented CUA-2 applications has been completed in a few person-weeks. This result demonstrates the power and productivity of the ITS approach and tools. The results of this case study showed that key ‘ready-mades’ (e.g. named attribute groups) provided in the style designer's toolkit could be used intact, which is important for importing or exporting interaction techniques from one style to another style. The results generalize to other computer-literate designers who may want to use these tools to create other user interface styles. 相似文献
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The potential of a nickel-ceria-zirconia based catalyst was examined for autothermal reforming of n-dodecane, tetralin and their mixture into a hydrogen-rich product stream suitable for high-temperature fuel cells. n-Dodecane and tetralin were chosen as representative compounds for alkanes and bicyclic compounds in jet fuel. It was possible to reach conversions greater than 90% for both components, as long as sufficiently high oxygen concentrations were maintained in the feed. Tetralin gave larger yields of reforming products than n-dodecane. During the transient start-up phase of the reactor, large temperature excursions were observed, suggesting that the reaction starts with complete combustion of fuel, giving way to autothermal reforming after a few minutes on stream. These high-temperature excursions during reactor start-up are large enough to change the catalyst surface areas. Interestingly, the mixture of tetralin and n-dodecane did not behave as a linear combination of the two pure components, but showed reforming characteristics similar to pure tetralin. The non-linear behavior of the mixture provides a caveat that investigations of single component model compounds may not be able to capture the reforming behavior of more complex fuel mixtures. 相似文献