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排序方式: 共有563条查询结果,搜索用时 15 毫秒
81.
82.
B Wang J Boyer V Srikantan L Coney R Carrano C Phan M Merva K Dang M Agadjanan L Gilbert 《Canadian Metallurgical Quarterly》1993,12(9):799-805
DNA, or genetic, inoculation mimics aspects of attenuated vaccines in that synthesis of specific foreign proteins is accomplished in the host. These proteins can be processed and presented on the relevant major histocompatibility complex (MHC) antigens and ultimately become the subject of immune surveillance. Very recently, we have described the use of the new technology to generate immune responses in mice against the human immunodeficiency virus type 1 (HIV-1) envelope using a gp160 DNA construct. Further analysis of this technology specifically in regard to HIV vaccine design is clearly important. In this report, we describe the analysis of additional HIV constructs as immunogens in both mice and report the use of this genetic immunization technology in nonhuman primates. In these studies, successful seroconversion occurs in more than 70% of the mice following the second immunization with 100 micrograms of construct DNA; three and four immunizations result in routinely 100% seroconversion of the mice. Furthermore, the same strategy has successfully seroconverted primates following their second inoculation, resulting in the generation of both antiviral and neutralizing antibodies in this animal species. These studies are the first report of which we are aware that demonstrate successful immunization of nonhuman primates through genetic vaccination technology and the first to describe genetic immunization of primates against HIV antigens. This technology has relevance for the development of safe and efficacious immunization strategies against HIV because it provides for relevant antigen production in vivo without the use of infectious agents. 相似文献
83.
TG Phan J Estell G Duggin I Beer D Smith MJ Ferson 《Canadian Metallurgical Quarterly》1998,169(11-12):644-646
Kombucha tea is an alternative therapy that is gaining popularity as a remedy for a diverse range of ailments. We report two cases of symptomatic lead poisoning requiring chelation therapy in a married couple who had been drinking Kombucha tea for six months, brewing the tea in a ceramic pot. We postulate that acids in the tea eluted lead from the glaze pigment used in the ceramic pot, in a manner analogous to elution of lead from crystal decanters by wine and spirits. 相似文献
84.
The sensitivity of a simple root of a polynominal is finite and well defined. For a multiple root, however, the existing definition becomes infinite, even when the root displacements remain finite. In this letter, a well behaved function is proposed to define a multiple-root sensitivity. It is also shown that the new definition, which degenerates to the conventional definition under a special condition, is a meaningful measure of actual root displacements. 相似文献
85.
Quinton L Le Caer JP Phan G Ligny-Lemaire C Bourdais-Jomaron J Ducancel F Chamot-Rooke J 《Analytical chemistry》2005,77(20):6630-6639
The standard analytical procedure for screening the proteomic profile of a venom often relies on an appropriate combination of sample extraction, electrophoresis, reversed-phase high-performance liquid chromatography, mass spectrometry, and Edman degradation. We present in this study a new approach for venom screening based on Fourier transform mass spectrometry (FTMS) analysis directly on the crude venom. The venom chosen is a unique sample from Atractaspis irregularis, a species never studied at the molecular level previously. This snake belongs to the Atractaspidae family that is known to produce highly toxic venoms containing endothelin-like peptides called sarafotoxins (SRTXs). Nanoelectrospray-FTMS spectrum of the crude venom allowed the identification of 60 distinct compounds with molecular masses from 600 to 14,000 Da, which would have been impossible without the resolution of this kind of instrument. De novo sequencing within the entire venom confirmed the sequences of two new families of sarafotoxins, whose precursors had been cloned, and allowed the characterization of a third one. One particularly interesting point was that the propolypeptides appeared processed not in one unique compound, but rather in different length molecules ranging from 15 for the shorter to 30 amino acids for the longer. Moreover, our results clearly establish that in the case of A. irregularis only one copy of mature sarafotoxin emerges from each precursor, which is a totally different organization in comparison of other precursors of SRTXs. 相似文献
86.
BE Sumpio SM Phan FP Gasparro LI Deckelbaum 《Canadian Metallurgical Quarterly》1993,17(6):1010-6; discussion 1016-8
PURPOSE: Restenosis after balloon angioplasty or the intimal hyperplasia occurring at distal anastomoses of bypass grafts severely limits the long-term therapy for peripheral vascular disease. The aim of this study was to evaluate the application of psoralen photochemotherapy with ultraviolet A (UVA)-activated 8-methoxypsoralen (8-MOP) to suppress smooth muscle cell (SMC) proliferation in vitro by the formation of 8-MOP-DNA monoadducts and interstrand cross-links to inhibit DNA synthesis. METHODS: Bovine aorta SMC (2 x 10(4)/cm2) were treated with 8-MOP (0 to 1000 ng/ml) for 30 minutes, followed by UVA (2 joule/cm2) to determine the dose of 8-MOP and UVA that inhibits SMC proliferation. RESULTS: The results show that 8-MOP in the range 30 to 1000 ng/ml in combination with 2 joule/cm2 UVA inhibited SMC proliferation by 40% to 60% 3 days after treatment. In time course studies the growth of SMC treated with 100 ng/ml 8-MOP and 2 joule/cm2 UVA were monitored over 5 days, and this regimen was found to be cytostatic. SMC viability was confirmed by trypan blue exclusion. CONCLUSIONS: Our studies suggest that 8-MOP/UVA photochemotherapy may represent a novel approach to the control of localized SMC proliferation. 相似文献
87.
Networks and Spatial Economics - We revisit a dynamical system for solving variational inequalities. Under strongly pseudomonotone and Lipschitz continuous assumptions of the considered operator,... 相似文献
88.
Buhari Adamu Muhammad Ooi Chee-Pun Baskaran Vishnu Monn Phan Raphael CW Wong KokSheik Tan Wooi-Haw 《Multimedia Tools and Applications》2022,81(7):9151-9176
Multimedia Tools and Applications - The interest in real-time micro-expression recognition has increased with the current trend in human-computer interaction applications. Presently, there are... 相似文献
89.
Duong Huan Thanh Phan Hieu Chi Tran Tu Minh Dhar Ashutosh Sutra 《Neural computing & applications》2021,33(23):16425-16437
Neural Computing and Applications - Predicting the critical buckling loads of functionally graded material (FGM) plates using an analytical method requires solving complex equations with various... 相似文献
90.