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101.
Aída J. Velarde-Salcedo Alberto Barrera-Pacheco Samuel Lara-González Gabriela M. Montero-Morán Agustín Díaz-Gois Elvira González de Mejia Ana P. Barba de la Rosa 《Food chemistry》2013
Bioactive compounds present in foods could potentially have beneficial effects on human health. In this study we report the in vitro inhibitory capacity of peptides released from amaranth seed proteins after enzymatic digestion, against dipeptidyl peptidase IV (DPPIV); an enzyme known to deactivate incretins, hormones involved in insulin secretion. Other seeds, such as soybean, black bean, and wheat were also tested. The highest inhibition of DPPIV was observed with amaranth peptides released after simulated gastrointestinal digestion, showing an IC50 of 1.1 mg/mL in a dose-dependent manner. In silico tryptic digestion of amaranth globulins was carried out releasing peptides larger than 13 residues. Some of these peptides were used for the in silico prediction of their binding modes with DPPIV. Docking models showed that the possible mechanism of globulin peptides to inhibit DPPIV was through blocking the active dimer formation. Peptides were also found inside the major cavity where the natural substrates reach the catalytic site of the enzyme. This is the first report of the identification of inhibitory DPPIV peptides from amaranth hydrolysates and the prediction of their binding modes at the molecular level, leading to their possible use as functional food ingredients in the prevention of diabetes. 相似文献
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Marta Anna Szychlinska Francesca Maria Trovato Michelino Di Rosa Lucia Malaguarnera Lidia Puzzo Rosy Leonardi Paola Castrogiovanni Giuseppe Musumeci 《International journal of molecular sciences》2016,17(3)
Osteoarthritis is the most common human arthritis characterized by degeneration of articular cartilage. Several studies reported that levels of human cartilage glycoprotein chitinase 3-like-1 (CHI3L1) are known as a potential marker for the activation of chondrocytes and the progression of Osteoarthritis (OA), whereas lubricin appears to be chondroprotective. The aim of this study was to investigate the co-expression and co-localization of CHI3L1 and lubricin in normal and osteoarthritic rat articular cartilage to correlate their modified expression to a specific grade of OA. Samples of normal and osteoarthritic rat articular cartilage were analyzed by the Kellgren–Lawrence OA severity scores, the Kraus’ modified Mankin score and the Histopathology Osteoarthritis Research Society International (OARSI) system for histomorphometric evaluations, and through CHI3L1 and lubricin gene expression, immunohistochemistry and double immuno-staining analysis. The immunoexpression and the mRNA levels of lubricin increased in normal cartilage and decreased in OA cartilage (normal vs. OA, p < 0.01). By contrast, the immunoexpression and the mRNA levels of CHI3L1 increased in OA cartilage and decreased in normal cartilage (normal vs. OA, p < 0.01). Our findings are consistent with reports suggesting that these two glycoproteins are functionally associated with the development of OA and in particular with grade 2/3 of OA, suggesting that in the future they could be helpful to stage the severity and progression of the disease. 相似文献
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Rosa María Tremiño Teresa Real-Herraiz Viviana Letelier José Marcos Ortega 《International Journal of Applied Ceramic Technology》2022,19(4):2135-2147
The objective of this research is to study the effects produced by ternary binders which combine the addition of waste brick powder with fly ash, limestone, ground granulated blast furnace slag or waste glass powder in the microstructure and mechanical properties of mortars. In these ternary binders, the ordinary Portland cement was partially replaced by 10% of waste brick powder and 10% of another of the abovementioned additions. Mortars prepared with ordinary Portland cement without additions were also prepared. The microstructure was characterized with mercury intrusion porosimetry, electrical resistivity, and thermogravimetric analyses. Ultrasonic pulse velocity, compressive and flexural strengths were also determined. Mortars made using ternary binders with two active additions showed higher pore refinement and higher electrical resistivity at 250 days. Furthermore, their compressive strength and ultrasonic pulse velocity were relatively similar or even higher than that noted for reference specimens. 相似文献
107.
锁相放大器是用来测量微弱信号的专用仪器.即使噪音上千倍于被测信号,通过锁相放大器,也能得到精确结果.随着数字信号处理技术在仪器中的应用,可编程仪器变得越来越灵活.结合虚拟仪器的技术,论文通过对SR7265的硬件重新编程,在同一个硬件上实现了虚拟锁相放大器、虚拟频谱仪、虚拟阻抗计和虚拟半导体参数分析仪等等功能.本虚拟仪器采用了4层的逻辑结构.同样的功能也在一个FPGA板上成功地部署. 相似文献
108.
Study of thermodegradation and thermostabilization of poly(lactide acid) using subsequent extrusion cycles
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Nathalia S. Q. S. Amorin Gabriela Rosa Jefferson Fernandes Alves Suely P. C. Gonçalves Sandra M. M. Franchetti Guilhermino J. M. Fechine 《应用聚合物科学杂志》2014,131(6)
The thermomechanical degradation and thermostabilization of poly(lactic acid) (PLA) have been studied using subsequent extrusion cycles under different temperature profiles. Primary and secondary antioxidants were used to avoid degradation process during extrusion. Melt flow index (MFI), size exclusion chromatography (SEC), and infrared spectroscopy (FTIR) analysis were used to evaluate the degradation and stabilization of PLA. The MFI and SEC analysis show that the main thermodegradation mechanism of PLA is governed by scission reactions. FTIR analysis confirmed the SEC results and showed that the synergetic effect between primary and secondary antioxidant is a suitable way to thermostabilize the PLA. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2014 , 131, 40023. 相似文献
109.
Rosa Taurino Elena Fabbri Doris Pospiech Alla Synytska Massimo Messori 《Progress in Organic Coatings》2014
Organic–inorganic hybrid coatings on glass substrates with superhydrophobic properties and with improved scratch resistance were obtained by means of applying a multilayer approach including multiple sol–gel processes. The coatings exhibited a water contact angle (WCA) higher than 150°. Ultraviolet (UV)-curable vinyl ester resins and vinyltriethoxysilane (VTEOS) as coupling agent were employed to increase the adhesion between substrate and the inorganic layers. The surfaces were characterized by means of dynamic contact angle and roughness measurements. Indeed, the occurrence of superhydrophobic behavior was observed. The scratch resistance of the hybrid coatings was tested to evaluate the adhesion of the coatings to the glass substrate. The proposed preparation method for scratch resistant, mechanically stable, superhydrophobic coatings is simple and can be applied on large areas of different kinds of substrates. 相似文献