γ-氨基丁酸受体基因的系统性调控网络

提出一种多学科交叉结合的策略,试图初步获得系统性调控γ-氨基丁酸(gamma-aminobutyric acid,GABA)受体基因的转录因子线索.利用基于功能基因组学方法的DNA元件百科全书计划已发表的数据,系统性地获得GABA受体基因的开放染色质序列,并以此作为固相化探针,捕获与其特异性相互作用的蛋白质分子,并利用质谱分析鉴定蛋白.结果发现,对不同脑区获得的核蛋白,探针都能捕获到同样的特异性条带,然而,作为对照的天门冬氨酸(N-methyl-D-aspartate,NMDA)受体基因相关的开放染色质探针,在不同脑区中并未检获上述特异信号,说明结合蛋白是特异的.质谱分析表明,与GABA受体基因相关的开放染色质特异相互作用的蛋白是核膜血影重复蛋白-1(nuclear envelop spectrin repeatprotein-1,Nesprin-1),又称synaptic nuclear envelope-1(SYNE-1).进一步的调控网络生物信息学分析表明,Nesprin-1可能与MAFA、IRX2、BCL6、CEBPA以及RP58等转录因子形成复合物,并与GABA受体基因GABRA5、GABRA6、GABBR1和GABBR2等共表达.表明GABA受体基因在不同脑区是通过相同的转录调控机制进行表达的,Nesprin-1可能与MAFA、IRX2、BCL6、CEBPA以及RP58等转录因子形成复合物进而调控GABA受体基因表达,该特异的转录因子调控网络有望用于诱导多能干细胞或是前体细胞直接分化为GABA能神经元.     

Growth characteristics and kinetics of niobium carbide coating obtained on AISI 52100 by thermal-reactive diffusion technique

Niobium carbide coating was produced by thermal-reactive diffusion technique on AISI 52100 steel in salt bath at 1 123 K, 1 173 K, and 1 223 K for 1, 2, 4, and 6 hours. The salt consisted of borax, sodium fl uoride, boron carbide, and niobium pentoxide. The presence of NbC phase on the steel surface was confi rmed by X-ray diffraction analysis. Microscopic observation showed that niobium carbide coating formed on the substrate was smooth and compact. There was a distinct and fl at interface between the coating and substrate. The micro-hardness of niobium carbide coating was 2892±145HV. The thickness of coating ranged from 1.6 μm to 14μm. The forming kinetics of niobium carbide coating was revealed. Moreover, a contour diagram derived from experimental data was graphed for correct selection of process parameters. Some mathematical equations were built for predicting the coating thickness with predetermined processing temperature and time. The results showed that these mathematical equations are very practical as well as the kinetics equation.     

一类修正的q-Baskakov算子的逼近性质

Baskakov型算子是研究函数逼近问题的一种很重要的工具。该文基于Gupta所提出的q-Baskakov算子,介绍了一类修正的q-Baskakov算子,给出了该类算子的一些基本性质,并且得到了该类算子的一致收敛定理,从而进一步推广了q-Baskakov算子的一些结论。     

知识开源化背景下信息安全类课程教学改革初探

本文针对当前知识开源化的现状,结合信息安全类相关课程在北京电子科技学院授课的实际情况,以利用知识开源化所带来的优势,改善课程的教学效果、提高学生的动手实践能力为目的,研究和探讨了信息安全类部分课程的教学模式改革方式和所取得的效果。     

Effect of zirconia content and particle size on the properties of 3D-printed alumina-based ceramic cores

Alumina-based ceramic cores are used to manufacture the internal structures of hollow alloy blades, requiring both high precision and moderate properties. In this work, zirconia is regarded as a promoter to improve the mechanical properties of sintered ceramic. The effect of zirconia content and particle size on the microstructure and mechanical properties of ceramics was evaluated. The results indicate that the flexural strength of sintered ceramics reached the maximum of 14.5 ± 0.5 MPa when 20 wt% micron-sized (10 μm) zirconia (agglomerate size, consistent with the alumina particle size) was added, and 26.5±2.5 MPa when 15 wt% 0.3 μm zirconia was added. Zirconia with submicron-sized (0.3 μm) particles effectively filled the pores between alumina particles, thus leading to the maximum flexural strength with a relatively low content. The corresponding sintered ceramics had a bulk density of 2.0 g/cm³ and open porosity of 59.6%.     

Multiple Venting Behavior of Inner Dust Explosions

Combustion, Explosion, and Shock Waves - Explosion venting experiments of corn starch are carried out in a small-scale container. With the help of a high-speed camera and a pressure sensor, an...     

显微⁃傅里叶变换红外光谱鉴别分析微塑料

利用显微?傅里叶变换红外光谱技术分析鉴别了不同粒径及不同种类的微塑料（粒径小于5 mm的塑料、纤维或橡胶碎片）,系统阐述了反射、透射、衰减全反射3种测量模式及其微区成像技术在微塑料鉴别分析中的优缺点。基于显微?傅里叶变换红外光谱衰减全反射技术,分析了北京景观水样中的微塑料,结果表明该方法简单、准确、可靠。     

D杯形砂轮修整碟形金刚石砂轮试验研究

本文采用了D杯形砂轮与碟形砂轮对磨法来修整大直径树脂结合剂碟形金刚石砂轮。在分析研究杯形砂轮修整碟形砂轮的修整原理及修整方式的基础上，用自行研制的专用修整装置，从主轴转速、修整深度、修整工具结合剂类型等方面进行了对比工艺试验研究，总结了杯形砂轮修整碟形砂轮的不同工艺参数与修整效率、修整质量之间的工艺规律。试验结果表明，在本试验条件下主轴转速500r／min，修整深度0．02mm的修整效率较好；细粒度、中等浓度的青铜结合剂杯形金刚石砂轮与粗粒度、高浓度的杯形砂轮修整碟形砂轮相比，后者具有较好的修形效果和修整效率；D杯形砂轮与GC杯形砂轮交替配合使用可以大大提高碟形金刚石砂轮的修形效率和修锐效果。     

基于车磨试验台的超高速陶瓷CBN砂轮研究

本文论述了一个用于超高速磨削试验的陶瓷CBN砂轮的研制过程.通过ANSYS分析我们发现,对于砂轮基盘的材料,钛合金是本次试验最佳的选择;锥形是砂轮形状最佳的选择.基于分析结果和试验台的限制得出了砂轮基盘的精确截型.而且,根据试验数据,我们选择了30%的高韧性CBN500和70%的中等韧性CBN Ⅰ混合磨料及一种低温高强陶瓷结合剂.通过810℃烧结温度得到了理想的砂轮贴片.并经良好的粘结技术制出了用于200m/秒超高速磨削试验的CBN砂轮.最后,该砂轮的良好磨削性能得到了验证.     

高强度耐候钢焊接性分析与研究

通过对高强度耐候钢焊接性分析，采用与之匹配的焊接材料，对高强度耐候钢与不同钢种间焊接接头进行低温抗裂性、力学性能、耐大气腐蚀性能和焊接工艺性等进行试验，并对焊接接头金相组织进行分析，提出了高强度耐候钢焊接工艺及适宜的焊接材料。