Paracellular calcium transport across Caco-2 and HT29 cell monolayers

Intestinal calcium absorption has been shown to include two processes, a saturable transcellular movement and a non-saturable paracellular pathway. The potential utility of cell monolayers for studying transepithelial intestinal calcium transport has already been demonstrated; however, simultaneous evaluation of the contribution of the saturable transcellular and of the non-saturable paracellular processes to the total transepithelial transport has not yet been attempted. The aim of this study was to investigate the contribution both of transcellular and paracellular transport processes to the total transepithelial calcium transport in two cell culture monolayers. Caco-2 cells and a clone derived from HT-29 cells (HT29-Cl.19A), two cell lines derived from colon adenocarcinomas which are known to be able to exhibit typical enterocytic differentiation, were used. Cell monolayers were grown on a permeable support and used after 15 days of culture when these cells express enterocytic differentiation and high transepithelial resistance. Isotopic transport rate measurements were performed in the absence of a chemical gradient. The paracellular route was evaluated using [3H]mannitol. Calcium and [3H]mannitol transport rates across cell monolayers were not significantly different. Augmentation of calcium uptake by 200 mM sorbitol did not significantly increase calcium or mannitol transepithelial transport; however, calcium accumulation in the cells was increased by about 200%. Modulation of the monolayer permeability by addition of 10 nM vasoactive intestinal polypeptide (VIP) or 0.5 mM carbachol treatment, which respectively increased and decreased the transepithelial resistance, consequently modified calcium and mannitol transport in a parallel manner. Our results show that Caco-2 and HT29-Cl.19A cell monolayers are good models for studying the calcium paracellular transport pathway.     

ROLE OF COMPOSITION ON THE VISCOSITY AND SHEAR STRESS TEMPERATURE BEHAVIOUR OF SOME LUBE OIL BASE STOCKS

Lubricating oil base stocks differ greatly in their chemical composition and consequently in their physical properties and applications. Influence of waxes and various hydrocarbon type constituents viz saturates and aromatic components on the viscosity and shear stress as a function of temperature in different lube oil base stocks has been studied in the present paper.     

Mathematical analysis of dynamic channel selection in indoor mobilewireless communication systems

Dynamic channel selection (DCS) is an algorithm for flexible resource sharing in mobile radio systems. The digital enhanced cordless telecommunications (DECT) standard implements a version of DCS based on time as well as frequency multiplexing. In this paper, mathematical models are developed to evaluate the probabilities of channel availability, desired carrier power, and the carrier-to-interference ratio (CIR) for a constant traffic load. These results can be used to compute the call setup blocking probability. The models reported in this study are based on a decentralized DCS according to the DECT standard specifications. The results show that blocking due to availability of a channel (resource blocking), not interference blocking, generally is the most important factor in overall call blocking. Furthermore, it is seen that resource blocking is sensitive to the implementation of portable sets. Also, with homogeneous static traffic, this type of DCS ran be as good as fixed channel allocation (FCA) with respect to resource blocking     

The effect of multiple time scales and subexponentiality in MPEGvideo streams on queueing behavior

Guided by the empirical observation that real-time MPEG video streams exhibit both multiple time scale and subexponential characteristics, we construct a video model that captures both of these characteristics and is amenable to queueing analysis. We investigate two fundamental approaches for extracting the model parameters: using sample path and second-order statistics-based methods. The model exhibits the following two canonical queueing behaviors. When strict stability conditions are satisfied, i.e., the conditional mean of each scene is smaller than the capacity of the server, precise modeling of the interscene dynamics (long-term dependency) is not essential for the accurate prediction of small to moderately large queue sizes. In this case, the queue length distribution is determined using quasistationary (perturbation theory) analysis. When weak stability conditions are satisfied, i.e., the conditional mean of at least one scene type is greater than the capacity of the server, the dominant effect for building a large queue size is the subexponential (long-tailed) scene length distribution. In this case, precise modeling of intrascene statistics is of secondary importance for predicting the large queueing behavior. A fluid model, whose arrival process is obtained from the video data by replacing scene statistics with their means, is shown to asymptotically converge to the exact queue distribution. Using the transition scenario of moving from one stability region to the other by a change in the value of the server capacity, we synthesize recent queueing theoretic advances and ad hoc results in video modeling, and unify a broad range of seemingly contradictory experimental observations found in the literature. As a word of caution for the widespread usage of second-order statistics modeling methods, we construct two processes with the same second-order statistics that produce distinctly different queueing behaviors     

Itraconazole for treatment of oral candidosis in pediatric cancer patients

The objective of this study was to determine the frequency, degree of curvature, and the configuration of mesiobuccal and mesiolingual root canals of mandibular first molars. The degree of curvature and configuration of root canals creates some technical difficulties to the clinician during biomechanical preparation. Therefore this knowledge is essential for successful endodontic therapy. Six hundred and ninety-seven freshly extracted mandibular first molars were used in this study. After introducing 0.8 to 15 K reamers into the mesial root canals, the teeth were radiographed in buccolingual (clinical) and mesiodistal (proximal) directions. All samples showed curvatures at varying degrees in both views. There was a significant correlation between the degree of primary curvature of mesiobuccal and mesiolingual canals in clinical directions for total samples. When the primary curvature values of the mesial root canals were evaluated in Vertucci classification, the mesiobuccal and mesiolingual canals in Vertucci type VI were found as significantly correlated (r = 0.7173, p < 0.05). A significant correlation was seen between the secondary curvature values of clinical and proximal views of mesiolingual canal in Vertucci type VI (r = 0.9891, p < 0.05). The secondary curvature values in the clinical views of mesiobuccal and mesiolingual canals were found as significantly correlated in Vertucci type II.     

The functional block of TNF but not of IL-6 prevents bone loss in ovariectomized mice

BACKGROUND: In response to the increasing rate of skin cancer, particularly melanoma in the United States, the Environmental Protection Agency, the National Weather Service, the Centers for Disease Control and Prevention, National Association of Physicians for the Environment, and the American Academy of Dermatology, developed the Ultraviolet Index (UVI) to inform the public of the strength of the sun's rays and advise on methods for sun protection. OBJECTIVE: Our purpose was to evaluate the extent to which television stations and newspapers reported the UVI and assess the public's response to it. METHODS: To evaluate the effect of this effort, we surveyed television weather forecasters at 185 stations and examined weather pages in 54 newspapers in 58 cities that received the UVI reports. We also conducted a population probability telephone survey of 700 white adults (18 years of age and older) in these 58 cities. RESULTS: Seventy-one percent of the 169 stations that provided survey data for both 1994 and 1995 broadcast the UVI; 61% of newspapers reported the UVI. Nearly 64% of the 700 respondents (n = 445) had heard of the UVI. Of these respondents, 38% (n = 170) stated that they or their family changed their sun protection practices as a result of the UVI. CONCLUSION: The majority of television weather forecasters and newspapers reported the UVI. Most of the public was aware of the UVI, causing some to change sun protection practices. Further evaluation is required to maximize the effect of the UVI on sun protection practices.     

Object-oriented H.263 compatible video coding platform forconferencing applications

This paper describes a prototype video coding platform meant for the conception and testing of multimedia products such as next-generation videophones. The platform is largely based on ITU-T Recommendation H.263, with a number of additional object-oriented quality enhancement features which make it especially well suited for very low bit-rate coding of “head-and-shoulders” video material typical of real-time multimedia applications, video teleconferencing, and video telephony. These features consist of: (1) segmentation into objects of interest, (2) segmentation-based prefiltering, (3) model-assisted rate control, (4) adaptive vector quantization, and finally (5) segmentation-based postfiltering. In the spirit of Recommendation H.263, these enhancements are modular and can be selectively turned on or off, thereby enabling a wide variety of coding modes