Intracellular localization of samarium in the lactating mammary gland cells: ultrastructural and microanalytical study

The frequent use of some rare earths in the medical and industrial domains make us worry about their intracellular behavior into the body. Reason for which we have investigated the subcellular localization of one of these elements, the samarium, in the mammary gland of lactating female wistar rats using two very sensitive methods of observation and microanalysis, the transmission electron microscopy and the secondary ion mass spectrometry. The ultrastructural study showed the presence of electron dense deposits in the lactating mammary glandular epithelial cell lysosomes of the samarium-treated rats, but no loaded lysosomes were observed in those of control rats. The microanalytical study allowed both the identification of the chemical species present in those deposits as samarium isotopes ((152) Sm(+)) and the cartography of its distribution. Our results confirm the previous ones showing that lysosomes of the glandular epithelial cells are the site of the intracellular concentration of foreign elements such as gallium. The intralysosomal deposits observed in the mammary glandular cells of the samarium-treated rats are similar in their form and density to those observed with the same element in other varieties of cells, such as liver, bone marrow, and spleen cells. Our ultrastructural and microanalytical results and those obtained in previous studies allow deducing that the intralysosomal deposits are very probably composed of an insoluble samarium phosphate salt.     

GPU Shape Grammars

GPU Shape Grammars provide a solution for interactive procedural generation, tuning and visualization of massive environment elements for both video games and production rendering. Our technique generates detailed models without explicit geometry storage. To this end we reformulate the grammar expansion for generation of detailed models at the tesselation control and geometry shader stages. Using the geometry generation capabilities of modern graphics hardware, our technique generated massive, highly detailed models. GPU Shape Grammars integrate within a scalable framework by introducing automatic generation of levels of detail at reduced cost. We apply our solution for interactive generation and rendering of scenes containing thousands of buildings and trees.     

Finding Induced Paths of Given Parity in Claw-Free Graphs

The Parity Path problem is to decide if a given graph contains both an induced path of odd length and an induced path of even length between two specified vertices. In the related problems Odd Induced Path and Even Induced Path, the goal is to determine whether an induced path of odd, respectively even, length between two specified vertices exists. Although all three problems are NP-complete in general, we show that they can be solved in $\mathcal{O}(n^{5})$ time for the class of claw-free graphs. Two vertices s and t form an even pair in G if every induced path from s to t in G has even length. Our results imply that the problem of deciding if two specified vertices of a claw-free graph form an even pair, as well as the problem of deciding if a given claw-free graph has an even pair, can be solved in $\mathcal{O}(n^{5})$ time and $\mathcal{O}(n^{7})$ time, respectively. We also show that we can decide in $\mathcal{O}(n^{7})$ time whether a claw-free graph has an induced cycle of given parity through a specified vertex. Finally, we show that a shortest induced path of given parity between two specified vertices of a claw-free perfect graph can be found in $\mathcal {O}(n^{7})$ time.     

Cognitive conflict in human-automation interactions: a psychophysiological study

The review of literature in sociology and distributed artificial intelligence reveals that the occurrence of conflict is a remarkable precursor to the disruption of multi-agent systems. The study of this concept could be applied to human factors concerns, as man-system conflict appears to provoke perseveration behavior and to degrade attentional abilities with a trend to excessive focus. Once entangled in such conflicts, the human operator will do anything to succeed in his current goal even if it jeopardizes the mission. In order to confirm these findings, an experimental setup, composed of a real unmanned ground vehicle, a ground station is developed. A scenario involving an authority conflict between the participants and the robot is proposed. Analysis of the effects of the conflict on the participants' cognition and arousal is assessed through heart-rate measurement (reflecting stress level) and eye-tracking techniques (index of attentional focus). Our results clearly show that the occurrence of the conflict leads to perseveration behavior and can induce higher heart rate as well as excessive attentional focus. These results are discussed in terms of task commitment issues and increased arousal. Moreover, our results suggest that individual differences may predict susceptibility to perseveration behavior.     

The cascaded nature of lexical selection and integration in auditory sentence processing.

An event-related brain potential experiment was carried out to investigate the temporal relationship between lexical selection and the semantic integration in auditory sentence processing. Participants were presented with spoken sentences that ended with a word that was either semantically congruent or anomalous. Information about the moment in which a sentence-final word could uniquely be identified, its isolation point (IP), was compared with the onset of the elicited N400 congruity effect, reflecting semantic integration processing. The results revealed that the onset of the N400 effect occurred prior to the IP of the sentence-final words. Moreover, the factor early or late IP did not affect the onset of the N400. These findings indicate that lexical selection and semantic integration are cascading processes, in that semantic integration processing can start before the acoustic information allows the selection of a unique candidate and seems to be attempted in parallel for multiple candidates that are still compatible with the bottom-up acoustic input. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Relative hazard rate estimation for right censored and left truncated data

In medical research differences among treatment groups are a common focus of study. The concept of relative hazard rate is a tool for comparing two groups in terms of their difference in risk rates. A kernel estimator is proposed in the case where both samples are subject to left truncation and right censoring and an iid representation is obtained in this setup. The asymptotic distribution and the asymptotic mean squared error of the estimator are obtained. An application to the famous Channing House data set illustrates the theory. Research supported in part by MCyT Grants PB98-0182-C02-01 and BFM2002-00265 (ERDF support included) and XUGA Grant PGIDT00PX110501 PN for the first author and by the Ministry of the Flemish Community (Project BIL00/28, International Scientific and Technological Cooperation) and by the IAP research network nr P5/24 of the Belgian State (Federal Office for Scientific, Technical and Cultural Affairs) for the last two authors.     

Quantitative and reliable in vitro method combining scanning electron microscopy and image analysis for the screening of osteotropic modulators

The increased generation and up-regulated activity of bone resorbing cells (osteoclasts) play a part in the impairment of bone remodeling in many bone diseases. Numerous drugs (bisphosphonates, calcitonin, selective estrogen receptor modulators) have been proposed to inhibit this increased osteoclastic activity. In this report, we describe a pit resorption assay quantified by scanning electron microscopy coupled with image analysis. Total rabbit bone cells with large numbers of osteoclasts were cultured on dentin slices. The whole surface of the dentin slice was scanned and both the number of resorption pits and the total resorbed surface area were measured. Resorption pits appeared at 48 h and increased gradually up to 96 h. Despite the observation of a strong correlation between the total resorption area and the number of pits, we suggest that area measurement is the most relevant marker for osteoclastic activity. Osteotropic factors stimulating or inhibiting osteoclastic activity were used to test the variations in resorption activity as measured with our method. This reproducible and sensitive quantitative method is a valuable tool for screening for osteoclastic inhibitors and, more generally, for investigating bone modulators.     

Vibration analysis of rolling element bearings with various defects under the action of an unbalanced force

In this paper, a method based on the finite element vibration analysis is presented for defect detection in rolling element bearings with single or multiple defects on different components of the bearing structure using the time and frequency domain parameters. A dynamic loading model is proposed in order to create the nodal excitation functions used in the finite element vibration analysis as external loading. A computer code written in Visual Basic programming language with a graphical user interface is developed to create the nodal excitations for different cases including the outer ring, inner ring or rolling element defects. Forced vibration analysis of a bearing structure is performed using the commercial finite element package I-DEAS under the action of an unbalanced force transferred to the structure via a ball bearing. Time and frequency domain parameters such as rms, crest factor, kurtosis and band energy ratio for the frequency spectrum of the enveloped signals are used to analyse the effect of the defect location and the number of defects on the time and frequency domain parameters. The role of the receiving point for vibration measurements is also investigated. The vibration data for various defect cases including the housing structure effect can be obtained using the finite element vibration analysis in order to develop an optimum monitoring method in condition monitoring studies.     

Psychological constructs in driving automation: a consensus model and critical comment on construct proliferation

As automation in vehicles becomes more prevalent, the call for understanding the behaviour of the driver while driving an automated vehicle becomes more salient. Although a variety of driver behaviour models exist, and various psychological constructs have been said to be influenced by automation, an empirically testable psychological model of automated driving has yet to be developed. Building upon Stanton and Young's model of driving automation, this article presents an updated model of interrelated psychological constructs. The proposed model was created based upon a systematic literature search of driving automation papers and a subsequent quantification of the number of reported links between a selected set of psychological constructs. A secondary aim of this article is to reach consensus in the use of psychological constructs regarding driving automation. Henceforth special attention is paid to resolving the issue of construct proliferation.