To monitor cellular processes in individual cells, it is important to measure the concentrations of intracellular metabolites and to retrieve them for analysis. The use of functionalized polyelectrolyte microcapsules as intracellular sensors for in vivo reporting is persented. Capsules loaded with streptavidin-rhodamine, which was introduced into fibroblasts by electroporation, autonomously escaped from an endocytic compartment and efficiently recruited biotin-fluorescein from the cytosol. This work demonstrates the utility of polyelectrolyte microcapsules for intracellular capture of metabolites and eventually for drug delivery on an organismic level. 相似文献
The study aimed to investigate the utilisation of prebiotic D-tagatose by probiotic lactobacilli (L. rhamnosus GG, L. casei, L. acidophilus and L. fermentum) and enteric pathogens (E. coli and S. typhimurium) to determine synbiotic potential of D-tagatose and lactobacilli combination. The antimicrobial efficacy of the synbiotic preparations (D-tagatose with lactobacillus) was assessed against selected pathogens in co-culture assays. Evidently, D-tagatose supported growth of selected lactobacilli, especially the L. rhamnosus GG and L. casei, but not the enteric pathogens. The tested synbiotic preparations completely inhibited growth of both the pathogens, even in the presence of D-glucose in co-cultures. The well-diffusion assay demonstrated the presence of antimicrobial activity as recorded by drop of culture pH. The study substantiated that D-tagatose could be an effective prebiotic component for formulation of potential synbiotic combinations with L. rhamnosus GG or L. casei that can be used as an alternative to antibiotics against enteric pathogens. 相似文献
The present investigation studied the effects of enhancements on the learning, retention, transfer to the unlearned form, and use of Blissymbols in 40 normal 3-year-old children. The subjects, seen individually, learned either 12 standard Blissymbols (SBS) or the same 12 symbols in the enhanced form (EBS). The symbols were introduced with short explanations. The number of trials taken to reach > 90% correct identification, the number of symbols selected appropriately to complete a communicative act, the number of symbols correctly identified a week after the acquisition phase was completed, and the number of symbols correctly identified in the untrained form of Blissymbols were determined. The results demonstrated that the subjects learned EBS faster than SBS, remembered more EBS than SBS in the retention task, did not differ in the communicative use of SBS and EBS, and were affected more negatively when presented with SBS than EBS in a task where the untrained form was presented. The results are discussed in terms of how very young children might benefit more from an illustration system such as EBS than from an orthographic system such as SBS. 相似文献
Cu-44Ni-5Cr alloy has been subjected to thermomechanical treatment which consisted of plastic deformation of as-quenched material by 50, 65 and 80% reduction in thickness followed by ageing in the interval of 500 to 650 °C for various durations of time. Progress in age-hardening was studied by means of hardness measurement and X-ray diffraction studies. The wavelength of composition modulation and strain amplitude were measured. It was found that age-hardening was a result of interaction between spinodal decomposition and recovery processes. Prior deformation was found to enhance the kinetics of both spinodal decomposition and coarsening. It was concluded that this resulted from increased vacancy concentration and increased coherency strain in the cold-worked material. 相似文献
Heart is an important and hardest working muscular organ of the human body. Inability of the heart to restore normal perfusion to the entire body refers to cardiac failure, which then with symptoms results in manifestation of congestive heart failure (CHF). Impairment in systolic function associated with chronic dilation of left ventricle is referred as dilated cardiomyopathy (DCM). The clinical examination, surface electrocardiogram (ECG), chest X-ray, blood markers and echocardiography play major role in the diagnosis of CHF. Though the ECG manifests chamber enlargement changes, it does not possess sensitive marker for the diagnosis of DCM, whereas echocardiographic assessment can effectively reveal the presence of asymptomatic DCM. This work proposes an automated screening method for classifying normal and CHF echocardiographic images affected due to DCM using variational mode decomposition technique. The texture features are extracted from variational mode decomposed image. These features are selected using particle swarm optimization and classified using support vector machine classifier with different kernel functions. We have validated our experiment using 300 four-chamber echocardiography images (150: normal, 150: CHF) obtained from 50 normal and 50 CHF patients. Our proposed approach yielded maximum average accuracy, sensitivity and specificity of 99.33%, 98.66% and 100%, respectively, using ten features. Thus, the developed diagnosis system can effectively detect CHF in its early stage using ultrasound images and aid the clinicians in their diagnosis.
Wireless Networks - This paper proposes a new technique based on Direct Sequence Code Division Multiple Access for underwater acoustically wireless transmission with excessive transmission rate.... 相似文献