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81.
Landahl EC Antipova O Bongaarts A Barrea R Berry R Binder LI Irving T Orgel J Vana L Rice SE 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》2011,649(1):184-187
We describe an instrument to record x-ray diffraction patterns from diseased regions of human brain tissue by combining an in-line visible light fluorescence microscope with an x-ray diffraction microprobe. We use thiazine red fluorescence to specifically label and detect the filamentous tau protein pathology associated with Pick's disease, as several labs have done previously. We demonstrate that thiazine red-enhanced regions within the tissue show periodic structure in x-ray diffraction that is not observed in healthy tissue. One observed periodicity (4.2 ?) is characteristic of cross-beta sheet structure, consistent with previous results from powder diffraction studies performed on purified, dried tau protein. 相似文献
82.
The growth conditions, the composition and the structural, optical and electrical properties of thin films of CuGaSe2 and CuGaTe2 have been studied using “flash” and “slow” evaporation in vacuum. Single phase films, when analyzing the absorption coefficient, present several energy gaps. For CuGaSe2, they are 1.59, 1.66, 2.03 and 2.11 eV, for CuGaTe2 1.23 and 1.89 eV. Both the CuGaSe2 and CuGaTe2 evaporated films are p-type; the resistivities, carrier densities and mobilities are appropriate for thin film solar cells. 相似文献
83.
84.
Julian Gutirrez Jorge A. Prez Camilo Rueda Frank D. Valencia 《Electronic Notes in Theoretical Computer Science》2007,171(2):117
In this paper we present our first approach to model and verify biological systems using ntcc, a concurrent constraint process calculus. We argue that the partial information constructs in ntcc can provide a suitable language for such systems. We also illustrate how ntcc may provide a unified framework for the analysis of biological systems, as they can be described, simulated and verified using the elements provided by the calculus. 相似文献
85.
De la Torre C Illa I Faulkner G Soria L Robles-Cedeño R Dominguez-Perles R De Luna N Gallardo E 《Proteomics. Clinical applications》2009,3(4):486-497
The muscular dystrophies are a large and heterogeneous group of neuromuscular disorders that can be classified according to the mode of inheritance, the clinical phenotype and the molecular defect. To better understand the pathological mechanisms of dysferlin myopathy we compared the protein-expression pattern in the muscle biopsies of six patients with this disease with six patients with limb girdle muscular dystrophy 2A, five with facioscapulohumeral dystrophy and six normal control subjects. To investigate differences in the expression levels of skeletal muscle proteins we used 2-DE and MS. Western blot or immunohistochemistry confirmed relevant results. The study showed specific increase expression of proteins involved in fast-to-slow fiber type conversion (ankyrin repeat protein 2), type I predominance (phosphorylated forms of slow troponin T), sarcomere stabilization (actinin-associated LIM protein), protein ubiquitination (TRIM 72) and skeletal muscle differentiation (Rho-GDP-dissociation inhibitor ly-GDI) in dysferlin myopathy. As anticipated, we also found differential expression of proteins common to all the muscular dystrophies studied. This comparative proteomic analysis suggests that in dysferlin myopathy (i) the type I fiber predominance is an active process of fiber type conversion rather than a selective loss of type II fibers and (ii) the dysregulation of proteins involved in muscle differentiation further confirms the role of dysferlin in this process. 相似文献
86.
R Lo-Man P Rueda C Sedlik E Deriaud I Casal C Leclerc 《Canadian Metallurgical Quarterly》1998,28(4):1401-1407
Hybrid virus-like particles (VLP) were prepared by self-assembly of the modified porcine parvovirus (PPV) VP2 capsid protein carrying a CD8+ or CD4+ T cell epitope. Immunization of mice with a single dose of these hybrid pseudo-particles, without adjuvant, induced strong cytotoxic T lymphocyte and T helper (Th) responses against the reporter epitope. The Th response was characterized by a Th1 phenotype. We also analyzed in vitro the uptake mechanism of these parvovirus-like particles and the processing requirements associated with presentation by MHC molecules. Although previously shown to be presented by MHC class I molecules, these particles also enter very efficiently the MHC class II endocytic pathway, and behave as conventional exogenous antigens. Indeed, the processing of chimeric PPV:VLP was performed in endosomal/lysosomal acidic vesicles and the presentation of the foreign epitope carried by these particles was sensitive to brefeldin A and cycloheximide, showing that the foreign peptide was loaded on nascent MHC class II molecules. These results give some indication of how PPV:VLP can be presented by MHC class I and class II molecules, and underscore the wide potency of such VLP system to deliver foreign antigens for vaccine design. 相似文献
87.
A design-for-testability (DFT) methodology for switched-capacitor (SC) filters is presented, based on an architecture using some additional circuitry and providing extra capabilities for both off- and online test. A programmable biquad is used for on-chip comparison of the transfer functions for every filter stage. Test area overhead consists of the programmable biquad, a set of switches, and a finite-sequential-machine (FSM) control part. The design and implementation of an example filter are included to assess the potential usefulness of this approach 相似文献
88.
We studied changes during lactation in the relative concentration of individual gangliosides in human milk from mothers delivering preterm and term infants. The relative content of G(D3) was higher in colostrum than in mature milk, and tended to be higher in preterm colostrum than in term colostrum, whereas the relative content of G(M3) was higher in mature milk than in colostrum, and was also higher in term than in preterm milk. As G(D3) is usually detected in developing tissues whereas G(M3) is more abundant in mature tissues, these results suggest a relationship between the presence of individual gangliosides in human milk and immaturity of the mammary gland in mothers of preterm infants. 相似文献
89.
Raul Jimenez Zambrano Francisco A. Rubinelli Wim M. Arnoldbik Jatindra K. Rath Ruud E. I. Schropp 《Solar Energy Materials & Solar Cells》2004,81(1):73-86
A new band gap profile (exponential profile) for the active layer of the a-SiGe:H single junction cell has been designed and experimentally demonstrated. By computer simulations we show how bending the grading of the band gap in the i-layer contributes to the enhancement of the carrier collection, improving the fill factor and efficiency. The differences observed between experiments and simulations are studied using Rutherford Backscattering Spectrometry (RBS). The results highlight weak points during the deposition process, whose control enables us to bring together experimental and computational results. 相似文献
90.
Woods Steven Paul; Rippeth Julie D.; Conover Emily; Gongvatana Assawin; Gonzalez Raul; Carey Catherine L.; Cherner Mariana; Heaton Robert K.; Grant Igor 《Canadian Metallurgical Quarterly》2005,19(1):35
Methamphetamine (MA) dependence is associated with deficits in episodic verbal memory, but the cognitive mechanisms underlying such impairments are not known. The authors evaluated a component process model of episodic verbal memory in 87 persons with MA dependence (MA+) and 71 demographically similar non-MA-using controls (MA-). Compared with MA- controls, MA+ participants demonstrated deficient overall learning, free recall, and utilization of semantic clustering, as well as higher rates of repetitions and intrusions. No between-groups differences were evident on measures of serial clustering, retention, or recognition discrimination. Taken together, these findings indicate that MA dependence is associated with deficient strategic (i.e., executive) control of verbal encoding and retrieval, which is consistent with the sequelae of MA-related prefronto-striatal circuit neurotoxicity. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献