Biohythane production from organic waste: Recent advancements,technical bottlenecks and prospects

The availability of fossil fuels is a major factor that determines the economy of a country. However, possible exhaustion of fossil fuel deposits as well as increased pollution, and other adverse effects on the environment has prompted us to search for alternative fuels. This resulted in the development of hythane, a blend of hydrogen with methane, at concentrations of 10%–30%. The breakdown of organic substrates using sequential dark fermentation (DF) and anaerobic digestion (AD) leads to biohythane production. The quality and quantity of biohythane can be improved by altering the following aspects: selection, development, and/or genetic engineering of suitable microbial consortium; the use of cheap, appropriate substrates; improved design of bioreactors; and the implementation of two-stage fermentation system. This review focusses on the mechanism of biohythane production and the different aspects involved in increasing both its production rate and quality. A comparative study has also been done to demonstrate the superiority of biohythane over other biofuels.     

Rising sludge in secondary settlers due to denitrification

High suspended solids concentrations in settler effluents can be caused by rising sludge, which is the effect of flotation of solids by nitrogen gas resulting from biological denitrification. Many factors influence the nitrogen gas bubble evolution. The most important factor is the rate of biological denitrification. Factors like nitrogen gas solubility and oxygen concentration in settler influent only play a minor role. The hydraulic retention time in the bottom part of the settler is, for all practical purposes, so high that sufficient nitrogen gas will be generated at temperatures above 20°C, if the nitrate content in the influent to the settler is above the critical one. For temperatures around 20°C the critical nitrate-nitrogen concentration is 6–8 g NO₃⁻-N/m³. The best measure in order to avoid rising sludge is to denitrify the wastewater in the treatment process ahead of the settler.     

Microstructure and mechanical properties of the superalloy ATI Allvac 718Plus™

ATI Allvac^® 718Plus™ is a novel nickel-based superalloy, which was designed for heavy-duty applications in aerospace turbines. In the present study the high-resolution investigation techniques, atom probe tomography, electron microscopy and in situ high-temperature small-angle neutron scattering were used for a comprehensive microstructural characterization. The alloy contains nanometer-sized spherical γ′ phase precipitates (Ni₃(Al,Ti)) and plate-shaped δ phase precipitates (Ni₃Nb) of micrometer size. The precipitation kinetics of the γ′ phase can be described by a classical model for coarsening. The precipitation strongly influences the mechanical properties and is of high scientific and technological interest.     

Oxidation of Silicon Carbide Fibers During Static Fatigue in Air at Intermediate Temperatures

The static fatigue of SiC-based fiber bundles and single fibers has been examined in previous papers, with emphasis placed on the analysis of the stress–rupture time data, and on the modelling of delayed failure from slow crack growth. The present paper investigates the oxidation of the fibers during static fatigue, at temperatures in the intermediate temperature range (500°–800°C). Two oxidation-induced phenomena have been evidenced: the formation of a thin silica film at the surface of fibers and the delayed failure of fiber bundles and single filaments. The stress–rupture time data are interpreted with respect to the chemical and structural characteristics of fibers, and to the oxide film growth rate. The structural analysis of the fibers was carried out using scanning electron microscopy and Auger electron spectroscopy. Delayed failure was found to result from slow crack propagation from surface defects, as a result of the consumption of the free carbon at grain boundaries and the local stresses induced by the SiC→SiO₂ transformation at the crack tip. The respective contributions of these phenomena to static fatigue are discussed.     

System fault-tolerance analysis of COTS-based satellite on-board computers

Fault-tolerance analysis reveals possible system behavior under the influence of faults. Such analysis is essential for satellites where faults might be caused by space radiation and autonomous recovery is needed. In this paper we present a statistical simulation approach for fault-tolerance analysis of satellite On-Board Computers (OBCs) that are based on Commercial Off-The-Shelf (COTS) components. Since the logic level of COTS electronics is unknown to satellite designers, a new higher-level fault-tolerance analysis is required. We propose such technique that relies on OBC modeling and fault modeling, based on the modeling principle of Single-Event Upsets (SEUs). For the first time we can compare the efficiency of fault-tolerance techniques implemented in software and Field-Programmable Gate Array (FPGA). In addition, our approach enables to analyze system fault-tolerance at early development stages. In a case study the approach is applied to an OBC with a Microsemi SmartFusion SoC, that executes a satellite attitude control algorithm. The gained statistical simulation results enabled 50% reduction in the hardware overhead of the implemented memory scrubbing technique without loss in fault-tolerance. Our method revealed critical fault-tolerance drawbacks of the initial system design that could have lead to satellite mission failure.     

Phytochelatin synthase activity as a marker of metal pollution

The synthesis of phytochelatins is catalyzed by γ-Glu-Cys dipeptidyl transpeptidase called phytochelatin synthase (PCS). Aim of this study was to suggest a new tool for determination of phytochelatin synthase activity in the tobacco BY-2 cells treated with different concentrations of the Cd(II). After the optimization steps, an experiment on BY-2 cells exposed to different concentrations of Cd(NO₃)₂ for 3 days was performed. At the end of the experiment, cells were harvested and homogenized. Reduced glutathione and cadmium (II) ions were added to the cell suspension supernatant. These mixtures were incubated at 35 °C for 30 min and analysed using high performance liquid chromatography coupled with electrochemical detector (HPLC-ED). The results revealed that PCS activity rises markedly with increasing concentration of cadmium (II) ions. The lowest concentration of the toxic metal ions caused almost three fold increase in PCS activity as compared to control samples. The activity of PCS (270 fkat) in treated cells was more than seven times higher in comparison to control ones. K_m for PCS was estimated as 2.3 mM.     

Aligned nanowires and nanodots by directed block copolymer assembly

The directed self-assembly of block copolymers (BCPs) is a promising route to generate highly ordered arrays of sub-10 nm features. Ultradense arrays of a monolayer of spherical microdomains or cylindrical microdomains oriented parallel to the surface have been produced where the lateral ordering is guided by surface patterning and the lattice defined by the patterning can be commensurate or incommensurate with the natural period of the BCP. Commensurability between the two can be used to elegantly manipulate the lateral ordering and orientation of the BCP microdomains so as to form well-aligned arrays of 1D nanowires or 2D addressable nanodots. No modification of the substrate surface, aside from the patterning, was used, making the influence of lattice mismatch and pattern amplification on the size, shape and pitch of the BCP microdomains more transparent. A skew angle between incommensurate lattices, defining a stretching or compression of the BCP chains to compensate for the lattice mismatch, is presented.     

Proteomics identification of differentially expressed proteins in the muscle of dysferlin myopathy patients

The muscular dystrophies are a large and heterogeneous group of neuromuscular disorders that can be classified according to the mode of inheritance, the clinical phenotype and the molecular defect. To better understand the pathological mechanisms of dysferlin myopathy we compared the protein-expression pattern in the muscle biopsies of six patients with this disease with six patients with limb girdle muscular dystrophy 2A, five with facioscapulohumeral dystrophy and six normal control subjects. To investigate differences in the expression levels of skeletal muscle proteins we used 2-DE and MS. Western blot or immunohistochemistry confirmed relevant results. The study showed specific increase expression of proteins involved in fast-to-slow fiber type conversion (ankyrin repeat protein 2), type I predominance (phosphorylated forms of slow troponin T), sarcomere stabilization (actinin-associated LIM protein), protein ubiquitination (TRIM 72) and skeletal muscle differentiation (Rho-GDP-dissociation inhibitor ly-GDI) in dysferlin myopathy. As anticipated, we also found differential expression of proteins common to all the muscular dystrophies studied. This comparative proteomic analysis suggests that in dysferlin myopathy (i) the type I fiber predominance is an active process of fiber type conversion rather than a selective loss of type II fibers and (ii) the dysregulation of proteins involved in muscle differentiation further confirms the role of dysferlin in this process.     

An overview of the development of the first wall and other principal components of a laser fusion power plant

This paper introduces the JNM Special Issue on the development of a first wall for the reaction chamber in a laser fusion power plant. In this approach to fusion energy a spherical target is injected into a large chamber and heated to fusion burn by an array of lasers. The target emissions are absorbed by the wall and encapsulating blanket, and the resulting heat converted into electricity. The bulk of the energy deposited in the first wall is in the form of X-rays (1.0-100 keV) and ions (0.1-4 MeV). In order to have a practical power plant, the first wall must be resistant to these emissions and suffer virtually no erosion on each shot. A wall candidate based on tungsten armor bonded to a low activation ferritic steel substrate has been chosen as the initial system to be studied. The choice was based on the vast experience with these materials in a nuclear environment and the ability to address most of the key remaining issues with existing facilities. This overview paper is divided into three parts. The first part summarizes the current state of the development of laser fusion energy. The second part introduces the tungsten armored ferritic steel concept, the three critical development issues (thermo-mechanical fatigue, helium retention, and bonding) and the research to address them. Based on progress to date the latter two appear to be resolvable, but the former remains a challenge. Complete details are presented in the companion papers in this JNM Special Issue. The third part discusses other factors that must be considered in the design of the first wall, including compatibility with blanket concepts, radiological concerns, and structural considerations.