Biodegradable Thin Metal Foils and Spin‐On Glass Materials for Transient Electronics

Biodegradable substrates and encapsulating materials play critical roles in the development of an emerging class of semiconductor technology, generally referred as “transient electronics”, whose key characteristic is an ability to dissolve completely, in a controlled manner, upon immersion in ground water or biofluids. The results presented here introduce the use of thin foils of Mo, Fe, W, or Zn as biodegradable substrates and silicate spin‐on‐glass (SOG) materials as insulating and encapsulating layers, with demonstrations of transient active (diode and transistor) and passive (capacitor and inductor) electronic components. Complete measurements of electrical characteristics demonstrate that the device performance can reach levels comparable to those possible with conventional, nontransient materials. Dissolution kinetics of the foils and cytotoxicity tests of the SOG yield information relevant to use in transient electronics for temporary biomedical implants, resorbable environmental monitors, and reduced waste consumer electronics.     

Family B DNA polymerase from a hyperthermophilic archaeon Pyrobaculum calidifontis: cloning, characterization and PCR application

The 2352 bp gene coding for 783 amino acid family B DNA polymerase from Pyrobaculum calidifontis was cloned and expressed in Escherichia coli. Expression of the gene resulted in the production of Pca-Pol in soluble fraction. After heat denaturation of the host proteins, the Pca-Pol was further purified by ion exchange and hydrophobic interaction chromatographies. Activity gel analysis showed the presence of a catalytically active polypeptide of about 90 kDa. The mass of the protein, determined by Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry was found to be 89,156 Da. The isoelectric point of the enzyme was found to be 6.13. The optimal pH and magnesium ion concentration for the enzyme activity were 8.5 and 4mM, respectively. Unlike other commercially available DNA polymerases the enzyme activity of Pca-Pol was inhibited by monovalent cations such as ammonium and potassium. The half-life of the polymerase at 95 °C and 100 °C was 4.5h and 0.5h, respectively. The enzyme possessed 3'→5' exonuclease activity and was able to amplify, under suitable conditions, up to 7.5 kb DNA fragments by polymerase chain reaction which makes it a potential candidate for amplification of long DNA fragments.     

Development of a PCR-restriction fragment length polymorphism protocol for rapid detection and differentiation of four cockroach vectors (group I "Dirty 22" species) responsible for food contamination and spreading of foodborne pathogens: public health importance

Assessing the adulteration of food products and the presence of filth and extraneous materials is one of the measures that the U.S. Food and Drug Administration (FDA) utilizes in implementing regulatory actions of public health importance. To date, 22 common pest species (also known as the "Dirty 22" species) have been regarded by this agency as the spreaders of foodborne diseases. We have further categorized the Dirty 22 species into four groups: I has four cockroach species, II has two ant species, III has 12 fly species, and IV has four rodent species. The presence of any Dirty 22 species is also considered an indicator of unsanitary conditions in food processing and storage facilities. In this study, we describe the development of a two-step nested PCR protocol to amplify the small subunit ribosomal gene of group I Dirty 22 species that include four cockroach species: Blattella germanica, Blatta orientalis, Periplaneta americana, and Supella longipalpa, along with the development of a PCR-restriction fragment length polymorphism method for rapid detection and differentiation of these violative species. This method will be utilized when the specimen cannot be identified with conventional microscopic taxonomic methods, especially when only small body parts are separated and recovered from food samples for analysis or when these body parts are in a decomposed state. This new PCR-restriction fragment length polymorphism will provide correct identification of group I Dirty 22 species; this information can then be used in regulation and prevention of foodborne pathogens.     

Velocity field measurement of a round jet using quantitative schlieren

This paper utilizes the background oriented schlieren (BOS) technique to measure the velocity field of a variable density round jet. The density field of the jet is computed based on the light deflection created during the passage of light through the understudy jet. The deflection vector estimation was carried out using phase-based optical flow algorithms. The density field is further exploited to extract the axial and radial velocity vectors with the aid of continuity and energy equations. The experiment is conducted at six different jet-exit temperature values. Additional turbulence parameters, such as velocity variance and power spectral density of the vector field, are also computed. Finally, the measured velocity parameters are compared with the hot wire anemometer measurements and their correlation is displayed.     

Simplified gateway selection scheme for multihop relay in vehicular ad hoc network

Integrating vehicular ad hoc network (VANET) and universal mobile telecommunication system (UMTS) could be a promising architecture for future machine‐to‐machine applications. This integration can help vehicles have steady Internet connection through the UMTS network and in communicating with other vehicles. However, dead spot areas and unsuccessful handoff processes caused by the high speed of vehicles can disrupt the implementation of this kind of architecture. In this paper, a new simplified gateway selection (SGS) scheme for multihop relay in a VANET‐UMTS integration network is proposed. The proposed scheme extends the coverage or/and calming of the frequent handoff process and allows vehicles to continue to be connected to the UMTS infrastructure network. An integrated simulation environment that combines VanetMobiSim and NS2 is used to simulate and evaluate the proposed scheme. The simulation results show that the SGS scheme performed better when it was implemented with ad hoc on a demand distance vector routing and destination‐sequenced distance vector routing protocols. Furthermore, the SGS scheme is compared with other cluster‐based gateway selections used in the previous works. The results show that our SGS scheme outperforms other cluster‐based gateway selections scheme in terms of selection delay, control packet overhead, packet delivery ratio, and overall throughput. Copyright © 2013 John Wiley & Sons, Ltd.     

Polynomial approximation of shape function gradients from element geometries

A method is presented for the polynomial approximation of shape function gradients based solely on the geometry of finite element boundaries. The method is founded on a least squares approach which leads to an integration scheme satisfying a necessary condition for convergence. In its simplest form the method reduces to the well‐known uniform strain approach for finite elements. The method is applicable to a broad class of problems such as connecting dissimilar meshes, mesh adaptivity and transitioning, and the construction of finite elements with variable topologies. Finite elements based on the polynomial approximations are shown to pass patch tests of various orders. In contrast to standard elements, higher‐order patch tests are passed without the need for nodes internal to element boundaries. Less sensitivity to volumetric locking under plane strain conditions is demonstrated through comparisons with a standard element formulation. Copyright © 2001 John Wiley & Sons, Ltd.     

Flame development characteristics at variable swirl level inductions in a stratified CNG direct injection combustion engine

The study of flame development characteristics is crucial in the study of flame propagation, extinction, and for the investigation of combustion cyclic variability in SI engine. The aim of this study is to investigate the characteristics of flame development in a lean-stratified combustion of Natural Gas Engine （CNG） in a single cylinder direct injection （DI） engine at a specific motor speed, and fixed injection timing and air-fuel ratio by varying only the swirl level at the intake. The engine was set to run at 1800 rpm with half-load throttled. The ignition advance was set at 21.5 BTDC, and to create an overall lean and stratified mixture, injection timing was set at 61 BTDC with an air-fuel-ratio of 40.5 （λ=2.35）. Variable turbulent flow conditions near spark-plug were created by positioning the swirl control valves （SCV） at the intake port just before the two intake valves. This was done by setting one of the valves at full open position and the other one at 0% closed, 50% closed and 100% closed positions in order to achieve medium tumble （no swirl）, medium swirl and high swirl flows in the cylinder, respectively. An endoscope and CCD camera assembly was utilized to capture the flame images from the tumble plane at the intake side of the engine ever）, 2 CA degrees after ignition timing （AIT） for 40 CAs. It was observed that flame growth rate and flame convection velocity are increasing with increasing the swirl level. The total combustion duration is, thus, shorter in swirl induced combustion than without. However, COV in IMEP is greater in swirl induced flow cases than the medium tumble.     

Role of fever in infection: has induced fever any therapeutic potential in HIV infection?

Ancient societies had no rational understanding of fever. The Greeks were the first to recognise that it may be part of nature's method of effecting cure in some diseases. How best to assist nature went through many trials and errors. Appreciation of the prognostic value of fever and how it may be controlled was slow to appear. That there was a place in the therapeutic arsenal for induced fever came only with the 20th century. Finding a suitable, safe, and satisfactory means came slowly. The curative power of well controlled and reproducible levels of fever was proved by the arrest of one deadly and incurable complication of a sexually transmitted disease in the first half of this century. The purpose of this review is to promote discussion and, hopefully, well ordered laboratory and clinical trials aimed at learning whether or not induced fevers have a place in the care of patients with HIV/AIDS.     

Upon sector elements for two-dimensional problems

Different sector elements for two-dimensional problems for linear elastic analysis are developed and their utility for different types of problems is discussed. One of these elements (Type 1) fulfils the rigid body criterion for the two translatory motions as well as interelement compatibility conditions. This element is used together with a triangular element for the solution of Kirsch's problem. A considerable accuracy of the results is obtained with a relatively small number of elements.     

The relation between ketone bodies, glucose and minerals contents in bovine blood under various physiological conditions

The interrelationship between the concentration of ketone bodies, glucose, minerals (Na, K, Ca, Mg, P, Cl) in serum and in TCA soluble whole blood under different physiological conditions in 110 cows was investigated. There was a certain relationship (partly high significant) between the mineral content, ketone bodies and glucose levels in blood. However, there is no clear explanation for the qualification of blood minerals as an indicator of the content of ketone bodies and glucose in serum and whole blood. The age of animals and the seasons had no influence on the concentration of ketone bodies in serum and whole blood. Significant positive relationship was found between the time of lactation, pregnancy and serum glucose and negative relation with ketone bodies. There was no correlation between milk production and ketone bodies. The hours after feeding were positively correlated with ketone bodies.