Hancornia speciosa latex for biomedical applications: physical and chemical properties,biocompatibility assessment and angiogenic activity

The latex obtained from Hancornia speciosa is used in folk medicine for treatment of several diseases, such as acne, warts, diabetes, gastritis and inflammation. In this work, we describe the biocompatibility assessment and angiogenic properties of H. speciosa latex and its potential application in medicine. The physical–chemical characterization was carried out following different methodologies (CHN elemental analyses; thermogravimetric analyses and Fourier transform infrared spectroscopy). The biocompatibility was evaluated through cytotoxicity and genotoxicity tests in fibroblast mouse cells and the angiogenic properties were evaluated using the chick chorioallantoic membrane (CAM) assay model. The physical–chemical results showed that the structure of Hancornia speciosa latex biomembrane is very similar to that of Hevea brasiliensis (commercially available product). Moreover, the cytotoxicity and genotoxicity assays showed that H. speciosa latex is biocompatible with life systems and can be a good biomaterial for medical applications. The CAM test showed the efficient ability of H. speciosa latex in neovascularization of tissues. The histological analysis was in accordance with the results obtained in the CAM assay. Our data indicate that the latex obtained from H. speciosa and eluted in water showed significant angiogenic activity without any cytotoxic or genotoxic effects on life systems. The same did not occur with H. speciosa latex stabilized with ammonia. Addition of ammonia does not have significant effects on the structure of biomembranes, but showed a smaller cell survival and a significant genotoxicity effect. This study contributes to the understanding of the potentialities of H. speciosa latex as a source of new phytomedicines.     

SEM study of the morphology of asymmetric cellulose acetate membranes produced from recycled agro-industrial residues: sugarcane bagasse and mango seeds

Cellulose, obtained both from sugarcane bagasse and mango seeds, was used for synthesizing cellulose acetate in order to produce asymmetric membranes. These were compared to membranes of commercial cellulose acetate (Rhodia). All produced membranes were asymmetric, characterized by the presence of a dense skin and a porous support. Differences regarding the morphology of the surfaces as well as of the porous support can be noticed. Scanning Electron Microscopy (SEM) showed that the morphology of the superficial layer, responsible for transport, depends on the different lignin content of the starting material and also on the viscosity average molecular weight of the cellulose acetates produced from sugarcane bagasse, mango seed, and Rhodia’s commercial cellulose acetate.     

The rapid synthesis of nanostructured orthorhombic KNbO3 particles by a microwave-assisted hydrothermal method and their characterization

We studied the molar ratio effects of niobium and potassium precursors on the structure and morphology of potassium niobate powders prepared via microwave-assisted hydrothermal synthesis (MaHS). KNbO₃ nanostructures in the form of nanotowers and nanocubes were obtained at reduced synthesis times (30–240 min). The products were characterized via XRD, Raman spectroscopy, SEM, and TEM; band gap calculations used diffuse reflectance data. The results indicate that KNbO₃ nanostructures were obtained with crystallite sizes ranging from 33 to 52 nm. An orthorhombic crystalline structure was formed from the increase of KOH at a molar ratio Nb₂O₅:KOH (1:8 to 1:16 M). The band-gap of 3.1–3.3 eV has potential use in photodegradation applications.     

Structural cast-in-place SFRC: technology,control criteria and recent applications in spain

Steel fiber-reinforced concrete (SFRC) has been traditionally applied to pavements or tunnelling. However, classical structural applications have not been developed so fully. This paper examines different reasons which may justify the lack of this application. Several technologies on the fiber dosing system are presented and assessed. The SFRC reception control and acceptance criteria have been developed in accordance with the upcoming Spanish code. Adopting such criteria is justified on previous experiences and test viability under cast-in-place conditions. Some lines of action to promote the use of fibers with structural purposes are proposed. Two examples of recent applications are also set out, and a justification of the convenience of SFRC in terms of structural evaluation and durability under seawater conditions is provided.     

Study of strengthening solutions for glued-laminated wood beams of maritime pine wood

The application of the concept of glued-laminated wood (glulam) to improve the mechanical properties of the solid wood of the maritime pine (Pinus pinaster) is the subject of this work. Two reinforced glued-laminated wood beams are proposed and assessed. The first is based on a concept of laminated wood composite with fiber glass; the second is based on the application of pultruded lamellas glued to the most stressed tensile region of the glued-laminated beams. In order to demonstrate the potential of the proposed strengthening solutions, a comparison between their mechanical behaviors and the mechanical behaviors of the conventional glued-laminated and solid beams are performed. Static load–deflection curves, an equivalent Young’s modulus, the ultimate strain and modulus of rupture are determined using 3-point quasi-static bending tests. In general, it is demonstrated the beneficial effect of the proposed strengthening solutions both in terms of resistance and ductility.     

Forest fire indicators and mercury deposition in an intense land use change region in the Brazilian Amazon (Alta Floresta,MT)

Black carbon, charcoal and mercury fluxes were measured from sediment cores taken in an artificial water dam in an intense land use change area in the Alta Floresta district in the Brazilian Amazon, in order to characterize the differences in the evolution of human occupation patterns in the region during the last 18 years. A positive correlation between the black carbon and charcoal particle fluxes and the evolution of the Brazilian gross domestic production (GDP) was observed. Mercury fluxes showed a positive correlation with gold production and exhibited a distinct evolution pattern when compared to in relation to the forest fires indicators and Brazilian GDP. The fluxes of forest fires markers showed an increase in deforestation activities in the region after 1993. Mercury deposition showed a substantial decrease after 1994. The patterns of distribution in both forest fires tracers and gold mining tracers indicate substitution of the regional economic model. It also marked different antropogenic impact type in the ecosystem.     

A GRASP/VND heuristic for the phylogeny problem using a new neighborhood structure

A phylogeny is a tree that relates taxonomic units, based on their similarity over a set of characters. The phylogeny problem consists in finding a phylogeny with the minimum number of evolutionary steps. We propose a new neighborhood structure for the phylogeny problem. A greedy randomized adaptive search procedure heuristic based on this neighborhood structure and using variable neighborhood descent for local search is described. Computational results on randomly generated and benchmark instances are reported, showing that the new heuristic is quite robust and outperforms the other algorithms in the literature in terms of solution quality and time‐to‐target value.     

Verifying Object-based Graph Grammars

The development of concurrent and reactive systems is gaining importance since they are well-suited to modern computing platforms, such as the Internet. However, the development of correct concurrent and reactive systems is a non-trivial task. Object-based graph grammar (OBGG) is a visual formal language suitable for the specification of this class of systems. In previous work, a translation from OBGG to PROMELA (the input language of the SPIN model checker) was defined, enabling the verification of OBGG models using SPIN. In this paper we extend this approach in two different ways: (1) the approach for property specification is improved, enabling to prove properties not only about possible OBGG derivations, but also about the internal state of involved objects; (2) an approach is defined to interpret PROMELA races as OBGG derivations, generating graphical counter-examples for properties that are not true for a given OBGG model. Another contribution of this paper is (3) the definition of a method for model checking partial systems (isolated objects or a set of objects) using an assume-guarantee approach. A gas station system modeled with OBGGs is used to illustrate the contributions.This work is partially sponsored by projects IQ-MObile (CNPq-Brazil/CNR-Italy) and PLATUS (CNPq).Osmar Marchi dos Santos is partially sponsored by CAPES-Brazil.     

Reinforcement Learning Agents

Reinforcement Learning (RL) is learning through directexperimentation. It does not assume the existence of a teacher thatprovides examples upon which learning of a task takes place. Instead, inRL experience is the only teacher. With historical roots on the study ofbiological conditioned reflexes, RL attracts the interest of Engineersand Computer Scientists because of its theoretical relevance andpotential applications in fields as diverse as Operational Research andIntelligent Robotics.Computationally, RL is intended to operate in a learning environmentcomposed by two subjects: the learner and a dynamic process. Atsuccessive time steps, the learner makes an observation of the processstate, selects an action and applies it back to the process. Its goal isto find out an action policy that controls the behavior of the dynamicprocess, guided by signals (reinforcements) that indicate how badly orwell it has been performing the required task. These signals are usuallyassociated to a dramatic condition – e.g., accomplishment of a subtask(reward) or complete failure (punishment), and the learner tries tooptimize its behavior by using a performance measure (a function of thereceived reinforcements). The crucial point is that in order to do that,the learner must evaluate the conditions (associations between observedstates and chosen actions) that led to rewards or punishments.Starting from basic concepts, this tutorial presents the many flavorsof RL algorithms, develops the corresponding mathematical tools, assesstheir practical limitations and discusses alternatives that have beenproposed for applying RL to realistic tasks.     

Reliable indirect competitive ELISA used for a survey of ochratoxin A in green coffee from the North of Paraná State, Brazil.

The performance of an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) for ochratoxin A (OTA) detection was evaluated in a comparative study with high-performance liquid chromatography (HPLC) analysis using 68 freshly harvested coffee samples from the North of Paraná State, Brazil. The anti-OTA mAb showed high specificity and low cross-reactivity with OTA analogues (OTB and OTalpha), but cross-reacted with OTC. This ic-ELISA showed a detection limit of 3.75 ngg-1 sample, when compared to 0.80 ngg-1 by HPLC, with an ic-ELISA/HPLC correlation coefficient of 0.90. As regards OTA analysis of these coffee samples, natural contamination was detected in 10 samples (14.7%) by both methods, where the ic-ELISA values (range 3.9-7.3 ngg-1) were 1.1 to 1.6-fold higher than HPLC data (2.7-4.7 ngg-1). Five samples (7.4%) were OTA positive (range 0.84-1.30 ngg-1) only by HPLC assay, probably due to the higher detection limit reached by ic-ELISA. OTA was undetectable in 53 samples (77.9%) by both methods, while all positive samples (range 0.84-7.30 ngg-1) showed OTA levels lower than 8 ngg-1 (maximum limit recommended by the European Union). The matrix interference of green coffee was minimized by dilution of sample extracts before carrying out the ELISA assay. This mAb-based ic-ELISA can be effectively applied for OTA screening in coffee, because it is simple, sensitive and sample preparation is easy.