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While information on multidimensional constructs and empirical methods has become more accessible, there remain substantial challenges to theorizing about their form and implications. There are at least two ostensible reasons for such difficulties. First is the issue of terminology; many different terms are currently used to represent the same structural concept, and there is no evidence of standardization taking place around a single set of terms. Second, many studies do not clearly explain the theoretical reasons for choosing the specific multidimensional form of their constructs. To address these deficiencies, we use concepts from the research methods literature, and illustrations from the information systems (IS) literature, to review definitions and issues related to conceptualizing and operationalizing structural models that include multidimensional constructs. Such advice is necessary if we are going to develop and test increasingly sophisticated theoretical models in IS research. We also offer guidelines about how to conceptualize specific forms of multidimensional constructs. By lending greater conceptual clarity to the literature, we believe that this paper provides a foundation for future research incorporating multidimensional constructs in empirical analysis. 相似文献
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Pomegranate oil is the unique edible oil produced from the seed of the pomegranate fruit (Punica granatum L.), which can be extracted by various methods including supercritical CO2, superheated hexane, cold pressing, microwaves and ultrasound. The oil composition of pomegranate seed has received particular attention recently due to its content of antioxidant and anticancer agents, which may be important components for the production of new medicines and cosmetics. 相似文献
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The numbers of English public houses or “pubs” have reduced significantly in the last two decades. Politicians have called on the planning system to resist their closure but, at the same time, demand further controls over high-street bars. This paper explores the reasons for the decline and the rationale for supporting the continuation of the “traditional” English pub. Using evidence drawn from a wider study of the relationships between places and youth drinking cultures, the paper discusses young adults' use of pubs. While recognising the adverse effects of heavy drinking, arguments are put forward in support of the traditional pub as a site for restrained and responsible social interaction for young adults. The paper discusses the issues this raises for the UK planning system in the context of responsibilities for social sustainability and public health. 相似文献
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Mechanisms of Enhanced Catalysis in Enzyme–DNA Nanostructures Revealed through Molecular Simulations and Experimental Analysis
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Dr. Yingning Gao Christopher C. Roberts Aaron Toop Prof. Chia‐en A. Chang Prof. Ian Wheeldon 《Chembiochem : a European journal of chemical biology》2016,17(15):1430-1436
Understanding and controlling the molecular interactions between enzyme substrates and DNA nanostructures has important implications in the advancement of enzyme–DNA technologies as solutions in biocatalysis. Such hybrid nanostructures can be used to create enzyme systems with enhanced catalysis by controlling the local chemical and physical environments and the spatial organization of enzymes. Here we have used molecular simulations with corresponding experiments to describe a mechanism of enhanced catalysis due to locally increased substrate concentrations. With a series of DNA nanostructures conjugated to horseradish peroxidase, we show that binding interactions between substrates and the DNA structures can increase local substrate concentrations. Increased local substrate concentrations in HRP(DNA) nanostructures resulted in 2.9‐ and 2.4‐fold decreases in the apparent Michaelis constants of tetramethylbenzidine and 4‐aminophenol, substrates of HRP with tunable binding interactions to DNA nanostructures with dissociation constants in the micromolar range. Molecular simulations and kinetic analysis also revealed that increased local substrate concentrations enhanced the rates of substrate association. Identification of the mechanism of increased local concentration of substrates in close proximity to enzymes and their active sites adds to our understanding of nanostructured biocatalysis from which we can develop guidelines for enhancing catalysis in rationally designed systems. 相似文献
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Monitoring the performance of systems installed to protect against/halt the corrosion of reinforcing steel in concrete is essential in ensuring that the reinforcing bar (rebar) is adequately protected and remains in a non‐corroding state. A chloride contaminated concrete test block containing a hybrid anode system has been periodically monitored over the past 4.5 years to assess the anode current output and thus, the protection offered to the steel. De‐polarisation and impedance analysis data have been acquired to calculate steel corrosion rates. Both tests indicated that the steel was passive in the concrete environment and that the anode was able to protect all of the embedded steel despite the difficult geometry of the sample. It was also found that after 2 years it was still possible to drive a high current from the installed anode. 相似文献