Crystal growth in dental enamel: the role of amelogenins and albumin

Amelogenin-mineral interactions were investigated using an in vitro binding approach. Rat incisor enamel matrix proteins (mainly amelogenins) were dissolved in synthetic enamel fluid and allowed to equilibrate with deproteinised developing enamel crystals. The results showed that amlogenin proteins of 21, 23, 24, 26 and 27-kDa (corresponding to nascent and partially degraded amelogenins) were associated with the crystals whilst the lower Mr amelogenins (< 21 KDa) remained free in the synthetic enamel fluid. These data suggest the nascent and partially degraded amelogenins may interact with developing enamel crystals and could influence their growth. Albumin-mineral interactions were investigated by extracting developing rat incisor enamel with synthetic enamel fluid. Insoluble material (including the enamel crystals) was then further extracted with 0.1 M phosphate buffer (pH 7.4) to desorb any mineral bound proteins. Western blotting using anti-albumin antibodies showed that almost all of the albumin from the secretory stage enamel and a significant proportion of the albumin present in early transition stage was extractable in the synthetic enamel fluid. However, synthetic enamel fluid did not extract albumin from late transition or maturation stage tissue, which could only be removed following further extraction with phosphate buffer. Albumin degradation was apparent during the transition and maturation stages, where it is degraded and ultimately removed. This binding pattern may be related to amelogenin degradation and removal during the transition stage, permitting albumin access to the previously obscured crystal surfaces. That the secretory stage matrix appears to "protect" secretory stage crystals from albumin may be an important consideration in the aetiology of enamel hypoplasias (i.e. incomplete crystal growth) and when using dissociative extraction procedures for the identification of mineral bound proteins.     

A case study of a human reliability assessment for an existing nuclear power plant

This paper concerns a Human Reliability Assessment (HRA) carried out over a period of two years as part of a Nuclear Power Plant Probabilistic Safety Assessment (PSA). The HRA involved all elements of the HRA process, from problem definition and task analysis, through error representation and quantification, to impact assessment, error reduction, quality assurance and documentation. The aim of this paper is to show the methodology of HRA as applied in a real PSA, highlighting various elements of the HRA, and how the HRA interfaced with the PSA. Comments are also given on practical aspects and impacts of the various approaches, and the usefulness of a hybrid HRA/PSA team approach.     

Podcasting in education: Are students as ready and eager as we think they are?

Instructors in higher education are disseminating instructional content via podcasting, as many rally behind the technology’s potential benefits. Others have expressed concern about the risks of deleterious effects that might accompany the adoption of podcasting, such as lower class attendance. Yet, relatively few studies have investigated students’ perceptions of podcasting for educational purposes, especially in relation to different podcasting forms: repetitive and supplemental. The present study explored students’ readiness and attitudes towards these two forms of podcasting to provide fundamental information for future researchers and educators. The results indicated that students may not be as ready or eager to use podcasting for repetitive or supplemental educational purposes as much as we think they are, but they could be persuaded.     

Structure and Recognition of Graphs with No 6-wheel Subdivision

The subgraph homeomorphism problem has been shown by Robertson and Seymour to be polynomial-time solvable for any fixed pattern graph H. The result, however, is not practical, involving constants that are worse than exponential in |H|. Practical algorithms have only been developed for a few specific pattern graphs, the most recent of these being the wheels with four and five spokes. This paper looks at the subgraph homeomorphism problem where the pattern graph is a wheel with six spokes. The main result is a theorem characterizing graphs that do not contain subdivisions of W ₆. We give an efficient algorithm for solving the subgraph homeomorphism problem for W ₆. We also give a strengthening of the previous W ₅ result.     

Benchmarking stereo-derived DSMs to lidar by land cover and slope for resource development in northern Ontario,Canada

Topographic and elevation data are essential in the development of supporting infrastructure around mining sites. The de facto standard for acquiring elevation data is through light detection and ranging (lidar). The high labour and monetary cost of acquiring lidar has fostered more cost-effective approaches for creating elevation models that use stereo photogrammetry. To assess the accuracy of stereo-photogrammetry-derived elevation models and their potential application, we benchmark satellite (Worldview-2) and aircraft (South Central Ontario Orthoimagery Project; SCOOP) stereo-derived digital surface models (DSMs) against a lidar-derived DSM. Our results show that both stereo-derived DSMs have strong monotonic correlations with lidar across a range of land-cover types and slopes. The overall vertical accuracy of Worldview-2 and SCOOP DSMs are similar and do not meet the United States National Digital Elevation Program (NDEP) standards. However, accuracy assessment across land-cover types and slope categories show that specific land cover types (i.e. grass, row crops/pasture, sparse vegetation and marsh) on gently sloping terrain compare well to lidar data and meet NDEP accuracy standards. We situate the presented research in the context of northern resource development and discuss opportunities to improve the vertical accuracy of stereo-derived DSMs, for example, through unmanned aerial systems.     

Cloud motion in the GOCI/COMS ocean colour data

The Geostationary Ocean Colour Imager (GOCI) instrument, on Korea’s Communications, Oceans, and Meteorological Satellite (COMS), can produce a spectral artefact arising from the motion of clouds – the cloud is spatially shifted and the amount of shift varies by spectral band. The length of time it takes to acquire all eight GOCI bands for a given slot (portion of a scene) is sufficient to require that cloud motion be taken into account to fully mask or correct the effects of clouds in all bands. Inter-band correlations can be used to measure the amount of cloud shift, which can then be used to adjust the cloud mask so that the union of all shifted masks can act as a mask for all bands. This approach reduces the amount of masking required versus a simple expansion of the mask in all directions away from clouds. Cloud motion can also affect regions with unidentified clouds – thin or fractional clouds that evade the cloud identification process – yielding degraded quality in retrieved ocean colour parameters. Areas with moving and unidentified clouds require more elaborate masking algorithms to remove these degraded retrievals. Correction for the effects of moving fractional clouds may also be possible. The cloud shift information can be used to determine cloud motion and thus wind at the cloud levels on sub-minute timescales. The beneficial and negative effects of moving clouds should be considered for any ocean colour instrument design and associated data processing plans.     

An alternative objective function for fitting regression trees to functional response variables

Analyses of systems that can be represented by functional responses are becoming common in many scientific disciplines. Functional regression trees (FRT) provide a methodology for modelling such systems. Recent work has focused on fitting models where the response variable is a probability density function, using a splitting criterion that is based on the sum of dissimilarities between the densities. We suggest a different criterion based on deviations of the densities from their mean. We provide motivation and justification for this criterion, and demonstrate its superior performance using an extensive simulation exercise. We discuss the computational aspects of the FRT procedure and show that substantial speed gains can be made through use of a dissimilarity matrix. Our results show that the proposed splitting criterion outperforms both the original and a splitting criterion based on Euclidean distance. Pointwise standard error curves for a predicted functional response can be generated through the fitting procedure, which we demonstrate in a case study with a forestry data set. Supplementary materials are available.     

The Asynchronous Bounded-Cycle model

This paper shows how synchrony conditions can be added to the purely asynchronous model in a way that avoids any reference to message delays and computing step times, as well as system-wide constraints on execution patterns and network topology. Our Asynchronous Bounded-Cycle (ABC) model just bounds the ratio of the number of forward- and backward-oriented messages in certain (“relevant”) cycles in the space-time diagram of an asynchronous execution. We show that clock synchronization and lock-step rounds can be implemented and proved correct in the ABC model, even in the presence of Byzantine failures. Furthermore, we prove that any algorithm working correctly in the partially synchronous Θ-Model also works correctly in the ABC model. In our proof, we first apply a novel method for assigning certain message delays to asynchronous executions, which is based on a variant of Farkas’ theorem of linear inequalities and a non-standard cycle space of graphs. Using methods from point-set topology, we then prove that the existence of this delay assignment implies model indistinguishability for time-free safety and liveness properties. We also introduce several weaker variants of the ABC model, and relate our model to the existing partially synchronous system models, in particular, the classic models of Dwork, Lynch and Stockmayer and the query-response model by Mostefaoui, Mourgaya, and Raynal. Finally, we discuss some aspects of the ABC model’s applicability in real systems, in particular, in the context of VLSI Systems-on-Chip.