Efficient Sequence Alignment with Side-Constraints by Cluster Tree Elimination

Aligning DNA and protein sequences is a core technique in molecular biology. Often, it is desirable to include partial prior knowledge and conditions in an alignment. Going beyond prior work, we aim at the integration of such side constraints in free combination into alignment algorithms. The most common and successful technique for efficient alignment algorithms is dynamic programming (DP). However, a weakness of DP is that one cannot include additional constraints without specifically tailoring a new DP algorithm. Here, we discuss a declarative approach that is based on constraint techniques and show how it can be extended by formulating additional knowledge as constraints. We take special care to obtain the efficiency of DP for sequence alignment. This is achieved by careful modeling and applying proper solving strategies. Finally, we apply our method to the scanning for RNA motifs in large sequences. This case study demonstrates how the new approach can be used in real biological problems. A prototypic implementation of the method is available at .     

Quantification of the size-dependent energy gap of individual CdSe quantum dots by valence electron energy-loss spectroscopy

Valence electron energy-loss spectroscopy (VEELS) performed in a monochromated scanning transmission electron microscope was used to measure the energy gaps of individual quantum dots (QDs). The gap energies of a series of CdSe QDs measured by VEELS reveal the expected quantum confinement effect; the gap energy increases with decreasing particle size. However, the values derived from these first VEELS measurements of single QDs are larger than the values commonly measured by optical spectroscopy. As standard optical methods lack the spatial resolution to probe individual nanoparticles, the particle-size distribution influences the optical response. It is suggested that the impact of the particle-size distribution accounts for the discrepancy between the energy-gap values derived from VEELS of single QDs and from optical methods of ensembles of QDs.     

Muscular capabilities and workload of flight attendants for pushing and pulling trolleys aboard aircraft

Increasingly in the recent years, passengers’ services are extended into the ascent and descent flight phases on short distance flights. Trolleys containing the required meal and beverage items are used for these service operations and pushed or pulled along the aisles of the aircraft. Flight attendants reported about increased musculo-skeletal disorders and had been complaining about high physical workload from handling trolleys. In order to ensure acceptable load levels for pushing or pulling operations of trolleys, the physical capabilities of the collective “flight attendants” had been investigated by means of force measurements at maximum voluntary contraction (MVC) level and associated relevant anthropometrical and biometrical data. CEN and ISO standards as well as international and national German methods were used to derive recommended force limits for pushing/pulling operations with respect to the physical capabilities of the target group “flight attendants”. Comparing these recommended limits with the force requirements per shift under various conditions of trolley handlings (inclination of the floor, type and weight of trolley, mode of handling, frequency of operation) showed that especially for higher floor inclinations and trolley weights, flight attendants work (substantially) above recommended limits. It became also apparent that the handling of half-size trolleys is unexpectedly high demanding due to high vertical force components, caused by the unfavorable location of their center of gravity.

Relevance to industry

On short-distance flights, passengers’ services are performed by means of trolleys from the ascent to the descent flight phases. Flight attendants had been complaining about high physical workload. This study offers recommendations on maximum force limits for the handling of trolleys with respect to the muscular capacities and work situations of flight attendants on short-distance flights.     

Physical workload of flight attendants when pushing and pulling trolleys aboard aircraft

The musculoskeletal loads from moving trolleys aboard aircraft were assessed by observation of trolley handling on planes and by physical workload analyses of pushing and pulling of trolleys in a laboratory set up. Trolley handling by a total of 15 female flight attendants was observed on 10 short- and medium-distance flights in different types of aircraft. About 25 selected flight attendants (22 females; 3 males) of five German airlines took part in the laboratory study, which comprised three-dimensional (3D) measurements of posture and hand forces during pushing and pulling of trolleys in a variety of configurations. From the on-flight observations performed, between 150 and 250 trolley movements can be projected for a work shift. The greatest physical workload is to be expected at the beginning of service: The trolleys are fully laden then, and the cabin floor can still be inclined up to 8°, as the aircraft is still climbing, particularly on short-distance flights. The laboratory investigation revealed that the musculoskeletal workload from pushing and pulling depends essentially on the trolley load and on the gradient of the cabin floor. In addition, the degree of stressing depends significantly on the trolley type, mode of handling and personal dexterity. The up/down force component perpendicular to the direction of motion often achieved considerable amplitudes-in some cases equal to or exceeding the force in the direction of movement. The posture analysis demonstrated that pulling forced the flight attendants to adopt ergonomically unfavourable postures such as pronounced flexion of the back, particularly among female subjects. The highest values for flexion of the back occurred while pulling the half-size trolley. The results demonstrate that female flight attendants are likely to overload themselves if they frequently have to move heavily laden trolleys unaided on an inclined cabin floor.

Relevance to industry

On short distance flights, flight attendants have been complaining increasingly of high physical workload from manoeuvring trolleys. On the basis of the presented data airline companies may improve the trolley handling skills of their flight attendances by practical trainings and may ergonomically optimize the general service procedures aboard aircrafts.     

Time-of-flight sensor and color camera calibration for multi-view acquisition

This paper presents a multi-view acquisition system using multi-modal sensors, composed of time-of-flight (ToF) range sensors and color cameras. Our system captures the multiple pairs of color images and depth maps at multiple viewing directions. In order to ensure the acceptable accuracy of measurements, we compensate errors in sensor measurement and calibrate multi-modal devices. Upon manifold experiments and extensive analysis, we identify the major sources of systematic error in sensor measurement and construct an error model for compensation. As a result, we provide a practical solution for the real-time error compensation of depth measurement. Moreover, we implement the calibration scheme for multi-modal devices, unifying the spatial coordinate for multi-modal sensors. The main contribution of this work is to present the thorough analysis of systematic error in sensor measurement and therefore provide a reliable methodology for robust error compensation. The proposed system offers a real-time multi-modal sensor calibration method and thereby is applicable for the 3D reconstruction of dynamic scenes.     

Office task effects on comfort and body dynamics in five dynamic office chairs

In the present study, we investigated the effect of office tasks on posture and movements in field settings, and the comfort rating for chair characteristics and correlation with type of task. The tasks studied were: computer work, telephoning, desk work and conversation. Postures, movements, chair part inclinations and comfort rating data were collected from 12 subjects. Computer work showed the lowest physical activity, together with upright trunk and head position and low backrest inclination. Conversation shows the highest activity of head legs and low back together with the highest cervical spine extension. In contrast, desk work provoked the most cervical spine flexion and showed the second lowest activity. The telephoning tasks showed medium activity and the highest kyphosis. Conversation showed the highest backrest inclination. Positive comfort relations were found for computer work and a "swing system" chair, for telephoning and an active longitudinal seat rotation, and for desk work and a chair with a three-dimensionally moveable seat.     

Predicting Future Antisocial Personality Disorder in Males From a Clinical Assessment in Childhood.

It is essential to identify childhood predictors of adult antisocial personality disorder (APD) to target early prevention. It has variously been hypothesized that APD is predicted by childhood conduct disorder (CD), attention-deficit/hyperactivity disorder (ADHD), or both disorders. To test these competing hypotheses, the authors used data from a single childhood diagnostic assessment of 163 clinic-referred boys to predict future APD during early adulthood. Childhood Diagnostic and Statistical Manual of Mental Disorders (3rd ed., rev.; American Psychiatric Association, 1987) CD, but not ADHD, significantly predicted the boys' subsequent APD. An interaction between socioeconomic status (SES) and CD indicated that CD predicted APD only in lower SES families, however. Among children who met criteria for CD, their number of covert but not overt CD symptoms improved prediction of future APD, controlling for SES. (PsycINFO Database Record (c) 2010 APA, all rights reserved)