Endometrial Epithelial ARID1A Is Required for Uterine Immune Homeostasis during Early Pregnancy

A growing body of work suggests epigenetic dysregulation contributes to endometriosis pathophysiology and female infertility. The chromatin remodeling complex subunit AT-rich interaction domain 1A (ARID1A) must be properly expressed to maintain normal uterine function. Endometrial epithelial ARID1A is indispensable for pregnancy establishment in mice through regulation of endometrial gland function; however, ARID1A expression is decreased in infertile women with endometriosis. We hypothesized that ARID1A performs critical operations in the endometrial epithelium necessary for fertility besides maintaining gland function. To identify alterations in uterine gene expression resulting from loss of epithelial ARID1A, we performed RNA-sequencing analysis on pre-implantation uteri from Ltf^iCre/+Arid1a^f/f and control mice. Differential expression analysis identified 4181 differentially expressed genes enriched for immune-related ingenuity canonical pathways including agranulocyte adhesion and diapedesis and natural killer cell signaling. RT-qPCR confirmed an increase in pro-inflammatory cytokine and macrophage-related gene expression but a decrease in natural killer cell signaling. Immunostaining confirmed a uterus-specific increase in macrophage infiltration. Flow cytometry delineated an increase in inflammatory macrophages and a decrease in uterine dendritic cells in Ltf^iCre/+Arid1a^f/f uteri. These findings demonstrate a role for endometrial epithelial ARID1A in suppressing inflammation and maintaining uterine immune homeostasis, which are required for successful pregnancy and gynecological health.     

Structural Analysis of Mitochondrial Dynamics—From Cardiomyocytes to Osteoblasts: A Critical Review

Mitochondria play a crucial role in cell physiology and pathophysiology. In this context, mitochondrial dynamics and, subsequently, mitochondrial ultrastructure have increasingly become hot topics in modern research, with a focus on mitochondrial fission and fusion. Thus, the dynamics of mitochondria in several diseases have been intensively investigated, especially with a view to developing new promising treatment options. However, the majority of recent studies are performed in highly energy-dependent tissues, such as cardiac, hepatic, and neuronal tissues. In contrast, publications on mitochondrial dynamics from the orthopedic or trauma fields are quite rare, even if there are common cellular mechanisms in cardiovascular and bone tissue, especially regarding bone infection. The present report summarizes the spectrum of mitochondrial alterations in the cardiovascular system and compares it to the state of knowledge in the musculoskeletal system. The present paper summarizes recent knowledge regarding mitochondrial dynamics and gives a short, but not exhaustive, overview of its regulation via fission and fusion. Furthermore, the article highlights hypoxia and its accompanying increased mitochondrial fission as a possible link between cardiac ischemia and inflammatory diseases of the bone, such as osteomyelitis. This opens new innovative perspectives not only for the understanding of cellular pathomechanisms in osteomyelitis but also for potential new treatment options.     

Multi-cue hand detection and tracking for a head-mounted augmented reality system

With the recent developments in wearable augmented reality (AR), the role of natural human–computer interaction is becoming more important. Utilization of auxiliary hardware for interaction introduces extra complexity, weight and cost to wearable AR systems and natural means of interaction such as gestures are therefore more desirable. In this paper, we present a novel multi-cue hand detection and tracking method for head-mounted AR systems which combines depth, color, intensity and curvilinearity. The combination of different cues increases the detection rate, eliminates the background regions and therefore increases the tracking performance under challenging conditions. Detected hand positions and the trajectories are used to perform actions such as click, select, etc. Moreover, the 6 DOF poses of the hands are calculated by approximating the segmented regions with planes in order to render a planar menu (interface) around the hand and use the hand as a planar selection tool. The proposed system is tested on different scenarios (including markers for reference) and the results show that our system can detect and track the hands successfully in challenging conditions such as cluttered and dynamic environments and illumination variance. The proposed hand tracker outperforms other well-known hand trackers under these conditions.     

Multifinality in implicit choice.

This research examines the possibility that people's choices in the service of an explicit focal goal may also reflect their tendency to fulfill implicit background goals and in that sense are multifinal. We carried out 5 experimental studies to investigate this notion. In Experiment 1, a primed implicit goal affected individuals' choices even when those avowedly served an explicit “focal” goal. Experiment 2 replicated this finding with a different type of implicit goals. Experiment 3 found that primed implicit goals had no effect on choices where the options that served them undermined the explicit goal. Experiment 4 found that a primed implicit goal served by a multifinal option does not privilege it over a unifinal option if that goal had been already attained by a different means. Finally, Experiment 5, via 3 types of control groups, showed that choices were affected by both the explicit and implicit goals in isolation, and they shifted toward multifinality when these goals were conjointly present. The discussion considers the integrative potential of the multifinality framework and its implications for a variety of phenomena in the domain of motivated cognition. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Remote plasma etching of titanium nitride using NF3/argon and chlorine mixtures for chamber clean applications

To avoid plasma induced erosion of chamber hardware, the application of remote plasma sources to activate the etch gases was introduced. We present results on the etch behaviour of titanium nitride (TiN) using mixtures of NF₃, Cl₂ and argon. The gas mixture was excited in a remote plasma source and then routed through a reaction chamber to study the etch behaviour of TiN samples which simulate the situation at the chamber walls. The dependency of the TiN etch rate on temperature, gas flow, composition and pressure was examined. While the temperature (studied in the range 25-300 °C) turned out to be the most sensitive parameter, the general etch rate was mainly dependent on the availability of atomic fluorine. Etch products and NF₃/Cl₂ dissociation have been monitored by quadrupole mass spectrometry and infrared spectroscopy. While NF₃ showed a high decomposition up to 96%, chlorine decomposition was not observed. However the addition of chlorine increased the etch rates up to 260% in the low pressure/low temperature regime. Surface effects of chlorine addition are indicated by X-Ray Photoelectron Spectrometry and REM surface analysis.     

A Fourth Order Bandpass Delta-Sigma Modulator with Digitally Programmable Passband Frequency

The design and implementation of a fourth order switched-capacitorbandpass delta-sigma modulator with digitally programmable passbandis described. The quantization noise null can be programmed from0.4 (0.2f_s) to 0.6(0.3f_s) in steps of 0.01 (f_s/200)by changing digital switch settings. This design enables theA/D conversion of a bandpass signal with digital tuning of thecenter frequency for application in systems such as a transceiverIF stage. The modulator IC measures 4.8mm² in a2µ m CMOS process and achieves an SNR of 47 and59 dB over a 0.01 bandwidth at sampling ratesof 2.358 MHz and 1.25 MHz, respectively.     

Inverted Organic Solar Cells with Sol–Gel Processed High Work‐Function Vanadium Oxide Hole‐Extraction Layers

For large‐scale and high‐throughput production of organic solar cells (OSCs), liquid processing of the functional layers is desired. We demonstrate inverted bulk‐heterojunction organic solar cells (OSCs) with a sol–gel derived V₂O₅ hole‐extraction‐layer on top of the active organic layer. The V₂O₅ layers are prepared in ambient air using Vanadium(V)‐oxitriisopropoxide as precursor. Without any post‐annealing or plasma treatment, a high work function of the V₂O₅ layers is confirmed by both Kelvin probe analysis and ultraviolet photoelectron spectroscopy (UPS). Using UPS and inverse photoelectron spectroscopy (IPES), we show that the electronic structure of the solution processed V₂O₅ layers is similar to that of thermally evaporated V₂O₅ layers which have been exposed to ambient air. Optimization of the sol gel process leads to inverted OSCs with solution based V₂O₅ layers that show power conversion efficiencies similar to that of control devices with V₂O₅ layers prepared in high‐vacuum.     

Numerical Simulation of Waveguides of Arbitrary Cross-Section

Finite difference equations are derived for the simulation of dielectric waveguides using an H_z-E_z formulation defined on a nonuniform triangular grid. The resulting equations may be solved as a banded eigenproblem for waveguide structures of arbitrary shape composed of regions of piecewise constant isotropic dielectric, and all transverse fields then computed from the solutions. Benchmark comparisons are presented for problems with analytic solutions, as well as a sample calculation of the propagation loss of a hollow Bragg fiber.     

Correction for ambiguous solutions in factor analysis using a penalized least squares objective

Factor analysis is a powerful tool used for the analysis of dynamic studies. One of the major drawbacks of factor analysis of dynamic structures (FADS) is that the solution is not mathematically unique when only nonnegativity constraints are used to determine factors and factor coefficients. In this paper, a method to correct for ambiguous FADS solutions has been developed. A nonambiguous solution (to within certain scaling factors) is obtained by constructing and minimizing a new objective function. The most common objective function consists of a least squares term that when minimized with nonnegativity constraints, forces agreement between the applied factor model and the measured data. In our method, this objective function is modified by adding a term that penalizes multiple components in the images of the factor coefficients. Due to nonuniqueness effects, these factor coefficients consist of more than one physiological component. The technique was tested on computer simulations, an experimental canine cardiac study using 99mTc-teboroxime, and a patient planar 99mTc-MAG3 renal study. The results show that the technique works well in comparison to the truth in computer simulations and to region of interest (ROI) measurements in the experimental studies.