Dynamics of cardiac and skeletal muscle lactate dehydrogenase activity following a single exposure to an alternating magnetic field]

Analysis of records of microscopic slides from 33 patients with pure squamous carcinoma of the bladder who underwent radical cystectomy and lymphadenectomy disclosed a 5-year survival rate of 48 per cent. This survival rate correlated closely with pathologic stage and histologic grade of tumor and was significantly better than for each corresponding stage of transitional cell carcinoma of the bladder. The favorable survival rate and reasonably low operative mortality rate support aggressive therapy of this uncommon lesion.     

Coronary venous arterialization: acute hemodynamic, metabolic, and chronic anatomical observations

Power-spectrum analysis of the electroencephalogram and inhaled-exhaled concentrations of halothane (when used), nitrous oxide, and carbon dioxide of 36 patients during surgical operations under general anesthesia were monitored. Electroencephalographic arousal reactions were detected in 24 patients and these were accompanied by irregular respirations in nine patients. Cardiac arrhythmias occurred following an arousal reaction in eight patients. The authors conclude that this electroencephalographic arousal phenomenon occurs frequently and deserves further investigation to define its clinical implications and how it might be avoided.     

Immunohistochemical localization of epidermal growth factor receptors, epidermal-growth-factor-like and transforming-growth-factor-alpha-like peptides in chicken ovarian follicles

The purpose of this study was to determine the presence of epidermal growth factor receptor and its potential ligands epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha) in the tissues of the maturing follicles in the ovary of laying ISA-Brown hens using peptide-specific immunohistochemical methods. Cryostat sections, 6-8 microns thick, were made from fresh-frozen tissues of F1-F4 (largest to fourth largest) and large white follicles and they were immunostained for epidermal growth factor receptor, epidermal growth factor or transforming growth factor alpha using specific polyclonal antibodies. The EGF receptor and both ligands were detected in the granulosa, theca interna and theca externa layers of the follicles. The EGF receptor was localized both in the plasma membrane and cytoplasm of all cell types. EGF was predominantly cytosolic, whereas TGF-alpha was found in the plasma membranes and perinuclear areas of all cell types. The concentration of the receptor and both ligands decreased with follicular maturation. This observation is consistent with our previous observation that the response to EGF and TGF-alpha decreases as follicles mature, and thus provides further evidence that the receptor or the ligands may have a regulatory role in avian ovarian function.     

Role of Chylamydia in the pathology of pregnancy and chronic gynecological diseases

For the elucidation of the role of chlamydial infection in pathological pregnancies, serological examination by the CFT with group-specific and species ornithosis antigen was carried out on 723 blood serum specimens from women with obstetrical pathology, 124 specimens from patients with various chronic gynecological diseases and 124 control blood serum specimens from women with nromal pregnancy and favourable obstetrical anamnesis. The complement-fixing antibody (CF) was detected in 72 (9.9%) serum specimens from women with obstetrical pathology and chronic gynecological diseases. No CF antibody was found in control sera. Negative results of the CFT were obtained with species ornithosis antigen in all sera. In parallel examinations of the blood serum specimens from women with obstetrical and gynecological pathology by the CFT and intradermal test, correlation of positive results was demonstrated in 62.5% of the patients. Among the women with positive reactions for chlamydial infection, in 52.8% thee pregnancy was pathological. Among 52 sero-positive pregnant women the death of the fetus was observed in the perinatal period in 21.2%. Among 111 babies born alive after previous pregnancies 55 babies (49.5%) died at the age under 1 year. Thus, chlamydial infection may possibly have causative relation to pathological pregnancy, death of the fetus in the perinatal period, and death of the babies under 1 year.     

Evaluation of treatment for the prevention of acute gastric lesions in stress, in rats

Since portal hypertension affects the gastric mucosa, leading to congestive gastropathy and thus to increased incidence of bleeding, it is one of the possible causes of increased permeability of gastric mucosal capillaries. The aim of this study was the quantitative assessment of the permeability of the gastric mucosal endothelial cells. Eight CCl4-induced cirrhotic rats and eight matched controls were subjected to i.v. injection of FITC-albumin, and a morphometric evaluation of fluorescence in serial histological sections of the gastric mucosa was performed by a video image analysis system. Fluorescence was found to be 0.351 +/- 0.01% of the area scanned in experimental animals versus 0.073 +/- 0.005% in controls, i.e. it was significantly increased by the treatment, which implies a significant endothelial leakage into the extravascular space.