全文获取类型
收费全文 | 513164篇 |
免费 | 8276篇 |
国内免费 | 1771篇 |
专业分类
电工技术 | 8479篇 |
综合类 | 2649篇 |
化学工业 | 76146篇 |
金属工艺 | 21461篇 |
机械仪表 | 17013篇 |
建筑科学 | 11476篇 |
矿业工程 | 2751篇 |
能源动力 | 13253篇 |
轻工业 | 36101篇 |
水利工程 | 5702篇 |
石油天然气 | 8360篇 |
武器工业 | 21篇 |
无线电 | 59787篇 |
一般工业技术 | 103323篇 |
冶金工业 | 85652篇 |
原子能技术 | 9545篇 |
自动化技术 | 61492篇 |
出版年
2022年 | 2822篇 |
2021年 | 4619篇 |
2020年 | 3552篇 |
2019年 | 4384篇 |
2018年 | 21086篇 |
2017年 | 20038篇 |
2016年 | 17419篇 |
2015年 | 5791篇 |
2014年 | 8506篇 |
2013年 | 22488篇 |
2012年 | 16201篇 |
2011年 | 25666篇 |
2010年 | 21204篇 |
2009年 | 20066篇 |
2008年 | 21781篇 |
2007年 | 22228篇 |
2006年 | 13518篇 |
2005年 | 13035篇 |
2004年 | 12286篇 |
2003年 | 12171篇 |
2002年 | 10910篇 |
2001年 | 10203篇 |
2000年 | 9690篇 |
1999年 | 10036篇 |
1998年 | 26971篇 |
1997年 | 18305篇 |
1996年 | 13862篇 |
1995年 | 10147篇 |
1994年 | 8911篇 |
1993年 | 8908篇 |
1992年 | 6235篇 |
1991年 | 5903篇 |
1990年 | 5868篇 |
1989年 | 5506篇 |
1988年 | 5213篇 |
1987年 | 4561篇 |
1986年 | 4460篇 |
1985年 | 5000篇 |
1984年 | 4583篇 |
1983年 | 4116篇 |
1982年 | 3799篇 |
1981年 | 3885篇 |
1980年 | 3567篇 |
1979年 | 3443篇 |
1978年 | 3432篇 |
1977年 | 3919篇 |
1976年 | 5225篇 |
1975年 | 2926篇 |
1974年 | 2727篇 |
1973年 | 2800篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
121.
Emna Ouni Sbastien Pyr dit Ruys Marie-Madeleine Dolmans Gaëtan Herinckx Didier Vertommen Christiani A. Amorim 《International journal of molecular sciences》2020,21(23)
Currently, the extracellular matrix (ECM) is considered a pivotal complex meshwork of macromolecules playing a plethora of biomolecular functions in health and disease beyond its commonly known mechanical role. Only by unraveling its composition can we leverage related tissue engineering and pharmacological efforts. Nevertheless, its unbiased proteomic identification still encounters some limitations mainly due to partial ECM enrichment by precipitation, sequential fractionation using unfriendly-mass spectrometry (MS) detergents, and resuspension with harsh reagents that need to be entirely removed prior to analysis. These methods can be technically challenging and labor-intensive, which affects the reproducibility of ECM identification and induces protein loss. Here, we present a simple new method applicable to tissue fragments of 10 mg and more. The technique has been validated on human ovarian tissue and involves a standardized procedure for sample processing with an MS-compatible detergent and combined centrifugation. This two-step protocol eliminates the need for laborious sample clarification and divides our samples into 2 fractions, soluble and insoluble, successively enriched with matrisome-associated (ECM-interacting) and core matrisome (structural ECM) proteins. 相似文献
122.
Emma Layton Anna-Marie Fairhurst Sam Griffiths-Jones Richard K. Grencis Ian S. Roberts 《International journal of molecular sciences》2020,21(23)
In eukaryotes, microRNAs (miRNAs) have roles in development, homeostasis, disease and the immune response. Recent work has shown that plant and mammalian miRNAs also mediate cross-kingdom and cross-domain communications. However, these studies remain controversial and are lacking critical mechanistic explanations. Bacteria do not produce miRNAs themselves, and therefore it is unclear how these eukaryotic RNA molecules could function in the bacterial recipient. In this review, we compare and contrast the biogenesis and functions of regulatory RNAs in eukaryotes and bacteria. As a result, we discovered several conserved features and homologous components in these distinct pathways. These findings enabled us to propose novel mechanisms to explain how eukaryotic miRNAs could function in bacteria. Further understanding in this area is necessary to validate the findings of existing studies and could facilitate the use of miRNAs as novel tools for the directed remodelling of the human microbiota. 相似文献
123.
124.
125.
126.
127.
128.
129.
130.