GELYMAC: a Macintosh application for calculating DNA fragment size from gel electrophoresis migration data

A program called GELYMAC takes data on the distances migrated by DNA fragments in a one-dimensional electrophoretic gel and, using a cubic-spline best-fit of marker fragment distance migrated versus molecular size, calculates the molecular sizes of the fragments. Written in the Rascal (Real-time Pascal) programming language, the program runs on the Macintosh family of microcomputers. Rapid entry of marker and experimental fragment migration data is afforded using a scroll bar system adjacent to a graphic representation of a gel. Output includes tabular listing of the data, graphic cartoons of the gel, and the fragment locations and molecular sizes for individual gel lanes, and the calibration curve used in data computations.     

Mechanism of void formation in cable covering

Solidification after extrusion of coaxial cables has been experimentally simulated. Solidification tests were performed with two HDPE grades and under several pressures; the extrusion velocity was varied in a suitable range so as to detect the critical velocity for void formation. A qualitative analysis of the void formation mechanism is carried out, and the predicted trends favorably compare with the critical velocity data.     

Phase Studies Concerning Sintering in Aluminas Doped with Ti4+

Previous solid state analyses of sintering in Ti⁴⁺-doped-commercial alumina are shown to be in error because a liquid phase exists in the appropriate region of the Al₂O₃−TiO₃−Na₂Ophase diagram at least by 1350^°C, a temperature lower than that at which "solid state" studies were conducted. It is suggested that liquid-phase sintering is a much more common occurrence than was realized formerly.     

The function of bleaching earths in the processing of palm,palm kernel and coconut oils

The results presented in the literature, which attempt to elucidate the mechanisms by which triglyceride oils are bleached by earths, are reviewed. The impact of this work and how the mechanistic proposals affect changes in oil properties are considered, with particular emphasis on the needs of the palm oil processor. Important properties include color, metals and phosphorus content and oxidative stability of the oil. Investigations made in our own laboratories have been aimed at elucidating the effect of varying physical and chemical properties of the bleaching earth on the quality of bleached and deodorized oils. Techniques used in this work are pore-size distribution, surface area, scanning and transmission electron microscopy and a variety of chemical and X-ray analysis methods. The ability to vary such parameters in montmorillonite clays by alteration of process conditions to give materials with specific performance characteristics is demonstrated. Comparisons are made between acid-activated montmorillonites and other clay types.     

Monitoring of human enteric viruses and coliform bacteria in waters after urban flood in Jakarta, Indonesia.

Floodwaters in Kampung Melayu village, Jakarta, Indonesia, as well as river water and consumable water (including groundwater and tap water) samples in flooded and non-flooded areas, were quantitatively analysed to assess occurrence of viruses and total coliforms and E. coli as bacterial indicators after flooding event. High numbers of enterovirus, hepatitis A virus, norovirus (G1, G2) and adenovirus were detected at high concentration in floodwaters and waters sampled from Ciliwung River which runs across metropolitan Jakarta and is used widely for agriculture and domestic purposes by poor residents. One out of three groundwater wells in the flooded area was contaminated with all viruses tested while no viruses were found in groundwater samples in non-flooded areas and tap water samples. The results revealed that human enteric viruses, especially hepatitis A virus and adenovirus, were prevalent in Jakarta, Indonesia. This study suggested that flooding posed a higher risk of viral infection to the people through contamination of drinking water sources or direct contact with floodwaters.