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61.
Khoshmanesh K Akagi J Nahavandi S Kalantar-zadeh K Baratchi S Williams DE Cooper JM Wlodkowic D 《Analytical chemistry》2011,83(8):3217-3221
Development of the dielectrophoretic (DEP) live cell trapping technology and its interfacing with the environmental scanning electron microscopy (ESEM) is described. DEP microelectrode arrays were fabricated on glass substrate using photolithography and lift-off. Chip-based arrays were applied for ESEM analysis of DEP-trapped human leukemic cells. This work provides proof-of-concept interfacing of the DEP cell retention and trapping technology with ESEM to provide a high-resolution analysis of individual nonadherent cells. 相似文献
62.
Khoshmanesh K Akagi J Nahavandi S Skommer J Baratchi S Cooper JM Kalantar-Zadeh K Williams DE Wlodkowic D 《Analytical chemistry》2011,83(6):2133-2144
Quantification of programmed and accidental cell death provides useful end-points for the anticancer drug efficacy assessment. Cell death is, however, a stochastic process. Therefore, the opportunity to dynamically quantify individual cellular states is advantageous over the commonly employed static, end-point assays. In this work, we describe the development and application of a microfabricated, dielectrophoretic (DEP) cell immobilization platform for the real-time analysis of cancer drug-induced cytotoxicity. Microelectrode arrays were designed to generate weak electro-thermal vortices that support efficient drug mixing and rapid cell immobilization at the delta-shape regions of strong electric field formed between the opposite microelectrodes. We applied this technology to the dynamic analysis of hematopoietic tumor cells that represent a particular challenge for real-time imaging due to their dislodgement during image acquisition. The present study was designed to provide a comprehensive mechanistic rationale for accelerated cell-based assays on DEP chips using real-time labeling with cell permeability markers. In this context, we provide data on the complex behavior of viable vs dying cells in the DEP fields and probe the effects of DEP fields upon cell responses to anticancer drugs and overall bioassay performance. Results indicate that simple DEP cell immobilization technology can be readily applied for the dynamic analysis of investigational drugs in hematopoietic cancer cells. This ability is of particular importance in studying the outcome of patient derived cancer cells, when exposed to therapeutic drugs, as these cells are often rare and difficult to collect, purify and immobilize. 相似文献
63.
This paper presents a methodology to identify robust operating regions through the selection of controllable factory variables, using discrete event simulation. A casting plant melt facility was used as an industrial test bed to develop these techniques. A robust system design was determined by response surface analysis of key production parameters. Furthermore, robust operating policies that maximise throughput, while minimizing work-in-progress and thus energy consumption were identified. 相似文献
64.
Frederick Chee Andrey V Savkin Tyrone L Fernando Saeid Nahavandi 《IEEE transactions on bio-medical engineering》2005,52(10):1625-1631
The theory of H infinity optimal control has the feature of minimizing the worst-case gain of an unknown disturbance input. When appropriately modified, the theory can be used to design a "switching" controller that can be applied to insulin injection for blood glucose (BG) regulation. The "switching" controller is defined by a collection of basic insulin rates and a rule that switches the insulin rates from one value to another. The rule employed an estimation of BG from noisy measurements, and the subsequent optimization of a performance index that involves the solution of a "jump" Riccati differential equation and a discrete-time dynamic programming equation. With an appropriate patient model, simulation studies have shown that the controller could correct BG deviation using clinically acceptable insulin delivery rates. 相似文献
65.
José C. García Juan A. Montiel-Nelson Saeid Nooshabadi 《Analog Integrated Circuits and Signal Processing》2009,60(1-2):35-42
This paper reports a series of symmetric high performance, low to full swing level converters (udld1-converter to udld5-converter) for recovering signal levels at the receiver end of the global interconnects with large capacitive loads. The proposed udld5-converter provides a matching receiver for the up-down low swing voltage driver (UDLD) signaling style for driving the global interconnect lines. When implemented on 0.13 μm CMOS 1.2 V technology, the udld5-converter performs 16% faster, reduces the energy per switching event by 4%, the energy-delay product by 19%, and the active area by 10%, when compared with a counterpart up low swing voltage driver (ULD) level converter (uld-converter). The proposed level converter receivers, each provide a different performance energy saving trade off. The paper also provides comparative performance evaluation of the various proposed level converters and uld-converter. 相似文献
66.
Influence of hydrothermal modification of fibers on some physical and mechanical properties of medium density
fiberboard (MDF) 总被引:1,自引:0,他引:1
Any treatment of fibers can influence properties of medium density fibreboard (MDF). In this research, the effects of hydrothermal treatment on physical and mechanical properties of MDF were studied. Industrial fibers were hydrothermally treated in a stainless steel reactor at 120, 150 and 180 °C for 0, 30 and 90 min as holding time. Test boards were made based on 0.7 g/cm3 target density, with 10 mm thickness under a pressure of 30 bar and at a temperature of 170 °C and a press time of 10 min. The boards were tested for internal bonding (IB), moduli of elasticity (MOE) and rupture (MOR), thickness swelling and water absorption. Results showed that the water absorption was not affected by the hydrothermal treatment; while the thickness swelling was improved and the boards became dimensionally stable. The MOE was slightly reduced due to the hydrothermal treatment. The MOR and the IB were significantly decreased by the hydrothermal treatment. 相似文献
67.
In this work, styrene‐maleic anhydride (St‐MA) copolymer was successfully grafted onto poly(vinyl chloride) (PVC) by means of chemical method in cyclohexanone medium. In this manner, the effects of various parameters such as total monomer content, monomers ratio, and initiator concentration on the grafting percentage (GP) and acid value (AV) were examined. The graft copolymers were characterized by Fourier‐transform infrared and nuclear magnetic resonance spectroscopy methods. Afterward, the cross‐linking reaction was carried out through MA hydrolysis and condensation reaction between maleic acid and produced diamines, by toluene diisocyanate (TDI) at the presence of hot water. The results showed that the GP and AV of PVC‐g‐(St‐MA) copolymers were considerably higher than those of PVC‐g‐MA and PVC‐g‐St with significant molecular weight. A gel content of 56% was attained with 1 phr TDI in PVC‐g‐(St‐MA) copolymer. The glass transition temperatures and mechanical properties of PVC‐g‐(St‐MA) samples were increased compared to pure PVC. Cross‐linked PVC‐g‐(St‐MA) showed improved mechanical properties than other samples, but the glass transition temperature of PVC backbone in this cross‐linked copolymer was disappeared due to its heavily dense structure. J. VINYL ADDIT. TECHNOL., 25:377–384, 2019. © 2019 Society of Plastics Engineers 相似文献
68.
Francesco Baino Elisa Fiume Jacopo Barberi Saeid Kargozar Juliana Marchi Jonathan Massera Enrica Verné 《International Journal of Applied Ceramic Technology》2019,16(5):1762-1796
Bioactive glasses exhibit the unique ability of bone bonding, thus creating a stable interface by stimulating bone cells toward mechanisms of regeneration and self-repair activated by ionic dissolution products. Therefore, 3D glass-derived scaffolds can be considered ideal porous templates to be used in bone tissue engineering strategies and regenerative medicine. This review provides a comprehensive overview of all technological aspects relevant to the fabrication of bioactive glass scaffolds, including the fundamentals of materials processing, a summary of the conventional porogen, and template-based methods and of recent additive manufacturing technologies, which are promising for large-scale production of highly reproducible and reliable implants suitable for a wide range of clinical applications. 相似文献
69.
70.
Telecommunication Systems - Queueing models play a significant role in analysing the performance of power management systems in various electronic devices and communication systems. This paper... 相似文献