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排序方式: 共有2875条查询结果,搜索用时 15 毫秒
21.
J Moreno Sierra ML Maestro de las Casas J Chicharro Almarza MD Ortega Heredia J López García-Asenjo C Merino Sánchez E Blanco Jiménez A Silmi Moyano L Rsesel Estévez 《Canadian Metallurgical Quarterly》1997,50(8):855-866
OBJECTIVE: The clinical course of transitional cell carcinoma of the bladder can be difficult to predict due to its potential to invade the muscle layer and/or develop to a high grade lesion. Bladder carcinoma can arise from genetic changes that may activate the oncogenes (-c-erbB2, c-erbB1, c-myc, ras, etc.) and/or inactivate the suppressor genes (p53, Rb). The aim of the present study is to continue a study protocol on the molecular biology of bladder tumors. METHODS/RESULTS: From January, 1993 to January, 1995, 85 patients were studied. These patients were divided into two groups: the first group comprised 14 controls of urothelial tissue and the second comprised 65 cases of transitional cell carcinoma of the bladder. p53 expression was determined by an immunohistochemical method (NCL-p53-DO7 monoclonal antibody). Quantification of the p8 oncoprotein in cytosol and EGFR (epidermal growth factor receptor) in membrane was performed by ELISA (Oncogene Science) and RIA (Vienna Lab), respectively. A statistically significant relationship between the expression of p53 and EGFR with tumor stage and grade was found. Quantification of p185 and EGFR showed higher values in the tumor tissue than in the control samples, but a worse survival could not be determined. CONCLUSIONS: The present study shows that p53 expression can be considered to be a prognostic factor. It provides useful information on the aggressive behaviour of the tumor and has a direct relation with the survival rates. 相似文献
22.
Liserre M. Pigazo A. Dell'Aquila A. Moreno V.M. 《Industrial Electronics, IEEE Transactions on》2006,53(5):1418-1426
The detection of the islanding condition of a distributed generation (DG) system is crucial for safety reasons, as discussed in the IEEE standards and specifically required by some national codes. Several anti-islanding methods that are resident in the inverter have been investigated and classified as passive (measurement of the natural effects of islanding) or active (based on the measurement of the effects due to transients or harmonics deliberately introduced in the system). In case the power drained by the load matches the power generated by the DG inverter, the effect of islanding is small, and the passive methods fail. However, the active methods, which have been developed to overcome these limits, create disturbances that can interact with those generated by other DG systems. In this paper, a new anti-islanding method is proposed. It exploits the natural sensitivity of a grid-voltage sensorless control to disturbances to highlight the islanding condition. The adopted grid-voltage sensorless control is adapted to a single-phase system with the use of resonant controllers based on the internal model control law: resonant-controller-based observer results. Then, a Kalman-filter-based algorithm is used to detect the islanding condition based on the energy mismatch between the estimated third and fifth harmonics and the real ones. Experimental results support the analysis 相似文献
23.
Nonisothermal thermogravimetry differential scanning calorimetry (TG‐DSC) mounting is intensively used for the determination of kinetic parameters and reaction heat along the chemical transformation of a solid. Nevertheless, when tests are performed with heating rates as high as those encountered in industrial processes, e.g., several tens of K min–1, there is great uncertainty in the knowledge of the exact sample temperature. In this work, a method to derive a simple mathematical expression is proposed and fully described in order to calculate the real sample temperature throughout a temperature‐ramped test on a commercial apparatus. The furnace temperature and the heat flow signals were used, together with the crucible specific heat and the heating rate. A number of validation tests were performed to derive similar reaction rates for a reference. First‐order kinetic reactions were presented and reconciled over a large range of heating rates from 3 to 50 K min–1. 相似文献
24.
Calaza C. Meca E. Marco S. Moreno M. Samitier J. Fonseca L. Gracia I. Cane C. 《IEEE sensors journal》2003,3(5):587-594
A model of a miniaturized non-dispersive infrared (NDIR) gas analysis system, aiming to predict the final system specifications, is presented. It comprises the different elements of the NDIR detector, including a surface micromachined Fabry-Perot tunable filter. These models have been used to estimate the response of the NDIR system to different gas mixtures. Multivariate regression methods like partial least squares allow recovering the true sample composition from the IR absorption spectra measured with the NDIR system, despite the limited selectivity of the filter. Combining model and data processing permits to predict the effect on the final system specification of design parameters. Here, we compare the effect of the technology used for the filter on the system errors. 相似文献
25.
Fabrication of alumina-aluminium titanate laminates that combine high strength external layers with flaw tolerant internal
layers is limited by the difficulty of the co-sintering of layers with large differences in the green state. This work describes
a new method to obtain alumina-aluminium titanate layered materials constituted by layers with large differences in terms
of grain size starting from green bodies with similar microstructures. The approach is based on the effect of small amounts
of titania (TiO2) as agents for alumina grain growth enhancement. Starting from a fine grained green body that combined alumina layers with
composite layers made of mixtures of alumina and titania, a two step sintering schedule led to a layered structure with external
layers of small grain sized alumina combined with internal alumina layers with large grain size due to the diffusion of titanium.
The large grain sized alumina layers conferred flaw tolerant behaviour to the laminate due to crack branching and bridging. 相似文献
26.
José Luis Montañez-Soto Salvador Alegret Juan Alfredo Salazar-Montoya Emma Gloria Ramos-Ramírez 《European Food Research and Technology》2006,223(3):379-386
In this work a novel amperometric biosensor for fructose determination in solutions was developed. The device was constructed by the incorporation of a tetrathiofulvalene-tetracyanoquinodimethane organic conducting salt and fructose dehydrogenase enzyme, include in a polymeric matrix of epoxy resin and graphite powder. Because of the electrocatalytic function of the salt, the direct transfer of the electron between the reduced prosthetic group (PQQH2) of the enzyme and the transducing material, was verified at a low working potential (150 mV vs. Ag/AgCl), where the interfering reactions were minimized. The response time at 90% of the steady state value was less than 20 s. The current response was directly proportional to the D-fructose concentration from 0.01 to 0.3 mmol/l with a detection limit of 0.005 mmol/l (signal/noise of 3) and a sensitivity of 1.9985 μA/mmol. The biosensor sensitivity diminishes when its surface is not polished between successive determinations, and remains constant (rsd=1.85, n=10) when the surface is polished between determinations. The effects of temperature and pH on the biosensor response were studied and analyzed; also the properties of the enzyme (Km
ap, I
max, Q10) were determinate in this work. The biosensor was used to determine fructose in high fructose syrups and there were not significant differences between these results and those obtained by HPLC (p≤0.05). During 4 months, in intermittent determinations the biosensor kept 100% of its original sensitivity and after 18 months stored at 4°C, it only lost 32% of its sensitivity. The simplicity, low working potential, high stability and good performance of this biosensor shows a great potential for its use in the fructose determination. 相似文献
27.
A Ruiz-Bravo M Jimenez-Valera A Sampedro E Moreno 《Canadian Metallurgical Quarterly》1994,9(4):317-324
The survival of Yersinia enterocolitica serotype O9 within mouse peritoneal macrophages was investigated. To evaluate the role of the virulence plasmid in the resistance to intracellular killing, an isogenic pair of virulent (plasmid-bearing) and avirulent (plasmid-less) O9 strains was used. The virulent strain was able to express plasmid-encoded outer membrane proteins and to colonize the Peyer's patches of orally infected mice. When mice were infected intraperitoneally, both strains were recovered at similar rates and over the same time from the peritoneal cavity. When in vitro assays were performed, both strains showed similar resistance to intracellular killing by monolayers of resident and inflammatory peritoneal macrophages. Previous opsonization of bacteria did not modify their survival within macrophage monolayers. We concluded that serotype O9 strains display a chromosome-mediated resistance to intracellular killing by mouse peritoneal macrophages. Moreover, macrophage resistance does not seem to be of importance for virulence of serotype O9 strains in mice. 相似文献
28.
An 18-year time series of monthly NOAA-AVHRR Pathfinder Land burned area was analyzed for the region of tropical Africa, from July 1981 to June 1999. The transition period between NOAA-11 and NOAA-14 platforms from July 1993 to June 1995 was not included due to missing and outlier data. Stability of the time series was addressed for the input variables in the burned area algorithm, reflectance and temperature channels.A Seasonal AutoRegressive Integrated Moving-Average (SARIMA) model was developed for forecasting potential burned area. The SARIMA model identified an autoregressive regular term with 1-month lag and an autoregressive 12-month seasonal term with one season (12 months) component. A cross-correlation between Southern Oscillation Index (SOI) and burned area was statistically significant predictor variable in a time series with 20-month lag. Results show that the SARIMA model with this predictor improved both, fitting and forecasting, residual variance, by 4.1% and 5.6%, respectively, thereby, demonstrating potential relationship between SOI and burned area for the study region. Forecasting was estimated by considering only the first 16 years of the monthly burned area in the time series, from July 1981 to June 1997. The prediction for the following 24 months (from July 1997 to June 1999) was within the 95% confidence level indicating that the forecast was a valid characterization of the modeled process. 相似文献
29.
Response time variability is a new optimization problem with a broad range of applications and a distinctive number of theoretic
flavour. The problem occurs whenever events, jobs, clients or products need to be sequenced so as to minimize the variability
of time for which they wait for the next turn in obtaining the resources necessary for their advance. The problem has numerous
real-life applications. We study its computational complexity, present efficiency, polynomial time algorithms for some cases,
and the NP-hardness proof for a general problem. We propose a position exchange heuristic and apply it to improve the total
response time variability of an initial sequence. The latter is the optimum bottleneck sequence, Webster or Jefferson sequence
of the apportionment, or a random sequence. We report on computational experiments with the heuristic. 相似文献
30.