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41.
Sandro Matosevic Nicolas Szita Frank Baganz 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2011,86(3):325-334
OVERVIEW: Over the last decade, the utility of immobilized microfluidic enzyme reactors (IMERs) has been demonstrated in a wide variety of fields, including medical diagnostics and therapy, biosensors, organic synthesis, drug discovery and many other applications. Of particular interest to the pharmaceutical industry is the potential for high throughput experimentation afforded by these systems, with a view to combinatorial synthesis for drug discovery applications. This article will focus on the current state of IMER systems, including immobilization techniques and microchannel flow generation, with a particular emphasis on applications and future prospects in view of likely directions and market potential of this field. IMPACT: The numerous advantages of attaching enzymes to a solid support, such as reuse of a single batch of enzyme, improved stability and durability, the ability to stop the reaction rapidly by removing the product from the reaction solution and the absence of enzyme contamination of the product are some of the attractive features of such systems. There are, however, a number of issues requiring careful consideration when developing such microsystems, including, but not limited to, surface modifications and exact control of fluid behaviour in microchannels, detection limitations, increased integration, and the reusability of the chips. APPLICATIONS: IMERs have received wide, including commercial, application as diagnostic tools for point‐of‐care applications, and, increasingly, as analytical tools in early drug development. Furthermore, peptide mapping and proteomics have employed IMER systems extensively over the past decade and growth in these areas continues. Copyright © 2011 Society of Chemical Industry 相似文献
42.
Heitor Luiz Ornaghi Jr. Alexandre Sonaglio Bolner Rudinei Fiorio Ademir Jose Zattera Sandro Campos Amico 《应用聚合物科学杂志》2010,118(2):887-896
This work aims to evaluate the performance of glass/sisal hybrid composites focusing on mechanical (flexural and impact) and dynamic mechanical analyses (DMTA). Hybrid composites with different fiber loadings and different volume ratios between glass and sisal were studied. The effect of the fiber length has also been investigated. The densities of the composites were compared with the theoretical values, showing agreement with the rule of mixtures. The results obtained in the flexural and impact analysis revealed that, in general, the properties were always higher for higher overall reinforcement content. By DMTA, an increase in the storage and loss modulus was found, as well as a shift to higher values for higher glass loading and overall fiber volume. It was also noticed an increase in the efficiency of the filler and the calculated activation energy for the relaxation process in the glass transition region. The fiber length did not significantly change the results observed in all analyses carried out in this work. The calculated adhesion factor increased for higher glass loadings, meaning the equation may not be applied for the system studied and there are other factors, besides adhesion influencing energy dissipation of the composites. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010 相似文献
43.
Alok Warey Sandro Balestrino Pat Szymkowicz M. R. Malayeri 《Aerosol science and technology》2013,47(2):198-213
Cooled exhaust gas recirculation (EGR) is widely used in diesel engines to control engine out NOx (oxides of nitrogen) emissions. A portion of the exhaust gases is recirculated into the intake manifold of the engine after cooling it through a heat exchanger. EGR cooler heat exchangers, however, tend to lose efficiency and have increased pressure drop as deposit forms on the heat exchanger surface. This adversely affects the combustion process, engine durability, and emissions. In this study, a 1-D model was developed to simulate soot deposition, soot removal, and condensation of several hydrocarbon (HC) species in a circular tube with turbulent gas flow at constant wall temperature. The circular tube, which makes up the computational domain in the model, represents a single channel from any EGR cooler geometry. The model takes into account soot particle deposition due to thermophoresis, diffusion, turbulent impaction, and gravitational drift. However, thermophoresis was found to be the most dominant deposition mechanism for boundary conditions at which EGR coolers typically operate. Soot removal was modeled by considering a force balance between the drag and van der Waals forces. A lognormal distribution of particles was assumed at the tube inlet. The evolution of the particle distribution in the bulk flow along the tube as well as the mass distribution in the deposit layer on the tube walls is predicted by the model. Condensation of six HC species between C15-C24 alkanes was also modeled. Predictions made by the model are in reasonably good agreement with experimental data obtained from a laboratory reactor under the same boundary conditions. There are several assumptions and simplifications built into the model, which can be refined further to improve it. Copyright 2012 American Association for Aerosol Research 相似文献
44.
45.
Fábio J.B. Brum Sandro C. Amico Ivo Vedana Jaime A. Spim 《Journal of Materials Processing Technology》2009,209(7):3166-3171
Autoclave processing is commonly used nowadays for dewaxing in the investment casting process. However, since the use of microwave is steadily growing in industrial processes and the wax interacts with the electromagnetic energy of the microwaves, the present work studies the possibility of carrying out dewaxing via microwave. The wax (mineral wax, vegetable resin, low molecular-weight polymer and anti-oxidant) used in this work was prepared by melting in an oven equipped with a mineral oil bath. The chemical and structural stability of the wax were monitored throughout 12 simulated dewaxing cycles, via various analyses, namely, volumetric expansion, hardness, Fourier transform infrared spectroscopy, ultraviolet–visible spectrophotometry, differential scanning calorimetry and viscosity. The results showed that microwave dewaxing is viable, significantly decreasing the incorporation of dirt and water, which is inevitable in the autoclave dewaxing process. 相似文献
46.
Tom Bocchino Sandro D‘Angelo 《电子产品世界》2001,(22):23-24,27
引言
ST最新推出的M50FW040固件集线器,采用了被称作‘低引脚数接口规范’或LPC的新主板互连协议。LPC总线是主板芯片组和单板外设功能之间的高速接口。…… 相似文献
47.
Protecting Research Subjects, Employees and Researchers: Implications for Software Engineering 总被引:1,自引:1,他引:0
Joan E. Sieber 《Empirical Software Engineering》2001,6(4):329-341
Human subjects research entails risks for subjects and, to a lesser extent, for researchers. This paper introduces the common types of risks to subjects of empirical software engineering research, and how risk can be a function of the context in which the research is conducted. Risks for researchers are also discussed. Government regulation of the ethics of human subjects research is introduced using the example of the Common Rule that governs much human subjects research taking place in the United States. 相似文献
48.
Sandro Montresor 《Industry and innovation》2008,15(3):321-346
The paper aims to investigate how innovations cluster in different technological systems (TSs) when their “techno‐economic”, rather than “territorial” space, is considered. Innovation clusters of economic sectors are identified by referring to the innovation “potential” represented by their R&D expenditure and by applying social network analysis to the intersectoral R&D flows matrices of 15 OECD countries in the mid‐1990s. Different clusterization models are first tested in order to detect the way sectors group on the basis of the embodied R&D flows they exchange. Actual clusters are then mapped in the different TSs by looking for intersectoral relationships which can be qualified to constitute “reduced TSs” (ReTSs). In all the 15 TSs investigated the techno‐economic space appears organized in hierarchies, along which its constitutive sectors grouped into clusters with different density and composition. Once ReTSs are looked for, the 15 TSs display highly heterogeneous structures, but with some interesting similarity on the basis of which different clusters of TSs can be identified in turn. 相似文献
49.
Development and Experimental Analysis of a Soft Compliant Tactile Microsensor for Anthropomorphic Artificial Hand 总被引:1,自引:0,他引:1
Beccai L. Roccella S. Ascari L. Valdastri P. Sieber A. Carrozza M.C. Dario P. 《Mechatronics, IEEE/ASME Transactions on》2008,13(2):158-168
This paper presents the development and preliminary experimental analysis of a soft compliant tactile microsensor (SCTM) with minimum thickness of 2 mm. A high shear sensitive triaxial force microsensor was embedded in a soft, compliant, flexible packaging. The performance of the whole system, including the SCTM, an electronic hardware and a processing algorithm, was evaluated by static calibration, maximum load tests, noise and dynamic tests, and by focusing on slippage experiments. A proper tradeoff between final robustness and sensitivity of the tactile device was identified. The experiments showed that the tactile sensor is sufficiently robust for application in artificial hands while sensitive enough for slip event detection. The sensor signals were elaborated with the cumulative summation algorithm and the results showed that the SCTM system could detect a slip event with a delay from a minimum of 24.5 ms to a maximum of 44 ms in the majority of experiments fulfilling the neurophysiological requirement. 相似文献
50.
Cinzia Ciccacci Sara Rufini Sandro Mancinelli Ersilia Buonomo Emiliano Giardina Paola Scarcella Maria C. Marazzi Giuseppe Novelli Leonardo Palombi Paola Borgiani 《International journal of molecular sciences》2015,16(3):5830-5838
Steven–Johnson Syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) are severe adverse drug reactions, characterized by extensive epidermal detachment and erosions of mucous membrane. SJS/TEN is one of the most serious adverse reactions to Nevirapine (NVP) treatment, commonly used in developing countries as first-line treatment of human immunodeficiency virus infection. In the last years TRAF3IP2 gene variants had been described as associated with susceptibility to several diseases such as psoriasis and psoriatic arthritis. We hypothesized that this gene, involved in immune response and in NF-κB activation, could also be implicated in the SJS/TEN susceptibility. We performed a full resequencing of TRAF3IP2 gene in a population of patients treated with NVP. Twenty-seven patients with NVP-induced SJS/TEN and 78 controls, all from Mozambique, were enrolled. We identified eight exonic and three intronic already described variants. The case/control association analysis highlighted an association between the rs76228616 SNP in exon 2 and the SJS/TEN susceptibility. In particular, the variant allele (C) resulted significantly associated with a higher risk to develop SJS/TEN (p = 0.012 and OR = 3.65 (95% CI 1.33–10.01)). A multivariate analysis by logistic regression confirmed its significant contribution (p = 0.027, OR = 4.39 (95% CI 1.19–16.23)). In conclusion, our study suggests that a variant in TRAF3IP2 gene could be involved in susceptibility to SJS/TEN. 相似文献