Porous methacrylate tissue engineering scaffolds: using carbon dioxide to control porosity and interconnectivity

Porous scaffold structures are used in tissue engineering to provide structural guidance for regenerating tissues. The use of carbon dioxide (CO₂) to create such scaffolds has received some attention in the past but many researchers believe that although CO₂ processing of polymers can lead to porous scaffolds there is limited interconnectivity between the pores. In this study, highly porous (greater than 85%) and well interconnected scaffolds were obtained in which the size, distribution and number of pores could be controlled. This control was achieved by altering the rate of venting from polymer discs saturated with CO₂ under modest temperature and pressure. The polymer used is a blend of poly (ethyl methacrylate) and tetrahydrofurfuryl methacrylate (PEMA/THFMA). This polymer system has shown promise for potential applications in cartilage repair.     

Sustainable Disposal of Domestic Sanitary Waste

The study presented is an example of the assessment of the relative sustainability of either option for disposal of domestic sanitary waste, either via the toilet or via the solid waste route. This required an evaluation of the total (social, economic, environmental, and technical) benefit/cost of implementing and adopting the alternative routes and an assessment of public responsiveness to encouragement to change sanitary waste disposal practices. It illustrates how, even for an apparently straightforward either/or question, the assessment of relative sustainability is complex and the amount of data needed to quantify sustainability indicators is prodigious. The study also provides an appraisal of the effectiveness of public campaigns to reduce waterborne disposal. Important information regarding public attitude and behavior in relation to wastewater systems has been acquired and lessons for ways of encouraging behavioral change to more sustainable ways of living have been gleaned.     

Characterizing and improving HIV/AIDS knowledge among cocaine-dependent outpatients.

The present study was an experimental test of efficacy of a brief education intervention for increasing HIV/AIDS knowledge among cocaine-dependent outpatients. Participants were randomly assigned to an HIV/AIDS education intervention (experimental condition) or a sham intervention (control condition). Control participants were subsequently crossed over to the HIV/AIDS education intervention. Experimental participants had higher scores on tests of HIV/AIDS knowledge after receiving the education intervention than did control participants. Further supporting the intervention's efficacy, control participant scores also increased once participants were crossed over and received the education intervention. Scores at follow-up were lower than at postintervention but remained higher than baseline scores. Results support the intervention's efficacy for increasing HIV/AIDS knowledge among cocaine-dependent outpatients. This intervention is brief, inexpensive, and easily implemented. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Software Process Improvement Problems in Twelve Software Companies: An Empirical Analysis

In this paper we discuss our study of the problems 12 software companies experienced in software development. In total we present qualitative data collected from 45 focus groups that involved over 200 software staff. We look at how different practitioner groups respond to software process improvement problems. We show our classification and analysis of this data using correspondence analysis. Correspondence analysis is a graphical data representation method new to software development research. The aim of the work we present is to develop a more holistic understanding of the problems practitioners are experiencing in their attempts to improve their software processes. Our main finding is that there is an association between a company's capability maturity and patterns of reported problems. Organizational problems are more associated with high maturity companies than with low maturity companies. Low maturity companies are closely linked to problems relating directly to projects such as documentation, timescales, tools and technology. Our findings also confirm differences in practitioner group problems. Senior managers cite problems with goals, culture and politics. Project managers are concerned with timescales, change management, budgets and estimates. Developers are experiencing problems with requirements, testing, documentation, communication, tools and technology. These associations are displayed graphically through correspondence analysis maps.     

Asymptotic Stabilisation of Multiple Input Nonlinear Systems via Sliding Modes

A dynamic feedback controller design method is proposed for multiple input systems. The method uses a novel choice of sliding surface to effect asymptotic linearisation of nonlinear differential input output systems and a class of state space systems. The stability of the overall system, that is a canonical state space form with a dynamic feedback, is analysed with a generalised Lyapunov approach plus an asymptotic analysis in a neighbourhood of the origin. The nonlinear system does not have to be expressed in regular form as is the case in many other sliding mode control approaches. A type of zero dynamics, which are the dynamics of the control, are involved. The resulting dynamic feedback is shown to provide chatter free control if the system is minimum phase with respect to the zero dynamics. The theoretical results are applied to Gas Jet systems with two controls.     

The Validity of the Five-Factor Model Prototypes for Personality Disorders in Two Clinical Samples.

The authors examined the validity of D. R. Lynam and T. A. Widiger's (2001) prototypes for personality disorders (PDs) derived from the facets of the 5-factor model (FFM) of personality in 2 clinical samples. In the 1st sample (N = 94), there was good agreement between the prototypes generated by experts and the profiles reported by patients. These FFM PD similarity scores also demonstrated good convergent and discriminant validity with results from a semistructured interview and a self-report measure of Axis II pathology. In the 2nd sample (N = 132), the FFM PD similarity scores demonstrated excellent longitudinal stability and good predictive validity with regard to consensus ratings of PD features. The implications of these findings are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A short course in antivirus software testing: seven simple rules for evaluating tests

Antivirus software tests are important when selecting antivirus software. However, there are many different tests, and interpreting the results can be challenging. Additionally, the needs of the corporate customer and home user differ, and it is important to understand these differences in order to evaluate antivirus software tests critically.     

Cold Atmospheric Plasma Jet Treatment Improves Human Keratinocyte Migration and Wound Closure Capacity without Causing Cellular Oxidative Stress

Cold Atmospheric Plasma (CAP) is an emerging technology with great potential for biomedical applications such as sterilizing equipment and antitumor strategies. CAP has also been shown to improve skin wound healing in vivo, but the biological mechanisms involved are not well known. Our study assessed a possible effect of a direct helium jet CAP treatment on keratinocytes, in both the immortalized N/TERT-1 human cell line and primary keratinocytes obtained from human skin samples. The cells were covered with 200 µL of phosphate buffered saline and exposed to the helium plasma jet for 10–120 s. In our experimental conditions, micromolar concentrations of hydrogen peroxide, nitrite and nitrate were produced. We showed that long-time CAP treatments (≥60 s) were cytotoxic, reduced keratinocyte migration, upregulated the expression of heat shock protein 27 (HSP27) and induced oxidative cell stress. In contrast, short-term CAP treatments (<60 s) were not cytotoxic, did not affect keratinocyte proliferation and differentiation, and did not induce any changes in mitochondria, but they did accelerate wound closure in vitro by improving keratinocyte migration. In conclusion, these results suggest that helium-based CAP treatments improve wound healing by stimulating keratinocyte migration. The study confirms that CAP could be a novel therapeutic method to treat recalcitrant wounds.     

A Novel Model Using AAV9-Cre to Knockout Adult Leydig Cell Gene Expression Reveals a Physiological Role of Glucocorticoid Receptor Signalling in Leydig Cell Function

Glucocorticoids are steroids involved in key physiological processes such as development, metabolism, inflammatory and stress responses and are mostly used exogenously as medications to treat various inflammation-based conditions. They act via the glucocorticoid receptor (GR) expressed in most cells. Exogenous glucocorticoids can negatively impact the function of the Leydig cells in the testis, leading to decreased androgen production. However, endogenous glucocorticoids are produced by the adrenal and within the testis, but whether their action on GR in Leydig cells regulates steroidogenesis is unknown. This study aimed to define the role of endogenous GR signalling in adult Leydig cells. We developed and compared two models; an inducible Cre transgene driven by expression of the Cyp17a1 steroidogenic gene (Cyp17-iCre) that depletes GR during development and a viral vector-driven Cre (AAV9-Cre) to deplete GR in adulthood. The delivery of AAV9-Cre ablated GR in adult mouse Leydig cells depleted Leydig cell GR more efficiently than the Cyp17-iCre model. Importantly, adult depletion of GR in Leydig cells caused reduced expression of luteinising hormone receptor (Lhcgr) and of steroidogenic enzymes required for normal androgen production. These findings reveal that Leydig cell GR signalling plays a physiological role in the testis and highlight that a normal balance of glucocorticoid activity in the testis is important for steroidogenesis.