A Standalone Burst-Mode Receiver With Clock and Data Recovery, Clock Phase Alignment, and RS(255, 239) Codes for SAC-OCDMA Applications

We demonstrate a standalone (no global clock) burst-mode receiver (BMRx) for a 7 times 622 Mb/s incoherent spectral-amplitude-coded optical code-division multiple-access system. The receiver provides the following functions: quantization (intensity noise filtering), clock and data recovery, burst-mode functionality (automatic phase acquisition) using a clock phase aligner (CPA), framing (for byte synchronization), and forward-error correction (FEC) using a (255, 239) Reed-Solomon decoder. The receiver provides an instantaneous (zero preamble bit) phase acquisition time for any phase step (plusmn2pi rads) between consecutive packets. With the CPA, we report a zero packet loss ratio (PLR) for up to four simultaneous users and more than a 300-fold improvement in the PLR for a fully loaded system. The BMRx also accomplishes more than 2.5 dB of coding gain, and achieves error-free (bit-error rate ) operation for a fully loaded system.     

Mechanically Defined Microenvironment Promotes Stabilization of Microvasculature,Which Correlates with the Enrichment of a Novel Piezo‐1+ Population of Circulating CD11b+/CD115+ Monocytes

Vascularization is a critical step in the restoration of cellular homeostasis. Several strategies including localized growth factor delivery, endothelial progenitor cells, genetically engineered cells, gene therapy, and prevascularized implants have been explored to promote revascularization. But, long‐term stabilization of newly induced vessels remains a challenge. It has been shown that fibroblasts and mesenchymal stem cells can stabilize newly induced vessels. However, whether an injected biomaterial alone can serve as an instructive environment for angiogenesis remains to be elucidated. It is reported here that appropriate vascular branching, and long‐term stabilization can be promoted simply by implanting a hydrogel with stiffness matching that of fibrin clot. A unique subpopulation of circulating CD11b⁺ myeloid and CD11b⁺/CD115⁺ monocytes that express the stretch activated cation channel Piezo‐1, which is enriched prominently in the clot‐like hydrogel, is identified. These findings offer evidence for a mechanobiology paradigm in angiogenesis involving an interplay between mechanosensitive circulating cells and mechanics of tissue microenvironment.     

3D Printed Solutions for Spheroid Engineering and Cancer Research

In multicellular organisms, cells are organized in a 3-dimensional framework and this is essential for organogenesis and tissue morphogenesis. Systems to recapitulate 3D cell growth are therefore vital for understanding development and cancer biology. Cells organized in 3D environments can evolve certain phenotypic traits valuable to physiologically relevant models that cannot be accessed in 2D culture. Cellular spheroids constitute an important aspect of in vitro tumor biology and they are usually prepared using the hanging drop method. Here a 3D printed approach is demonstrated to fabricate bespoke hanging drop devices for the culture of tumor cells. The design attributes of the hanging drop device take into account the need for high-throughput, high efficacy in spheroid formation, and automation. Specifically, in this study, custom-fit, modularized hanging drop devices comprising of inserts (Q-serts) were designed and fabricated using fused filament deposition (FFD). The utility of the Q-serts in the engineering of unicellular and multicellular spheroids-synthetic tumor microenvironment mimics (STEMs)—was established using human (cancer) cells. The culture of spheroids was automated using a pipetting robot and bioprinted using a custom bioink based on carboxylated agarose to simulate a tumor microenvironment (TME). The spheroids were characterized using light microscopy and histology. They showed good morphological and structural integrity and had high viability throughout the entire workflow. The systems and workflow presented here represent a user-focused 3D printing-driven spheroid culture platform which can be reliably reproduced in any research environment and scaled to- and on-demand. The standardization of spheroid preparation, handling, and culture should eliminate user-dependent variables, and have a positive impact on translational research to enable direct comparison of scientific findings.     

Significantly enhanced cocatalyst-free H2 evolution from defect-engineered Brown TiO2

TiO₂ is the extensively investigated materials for various photocatalytic reforming and water splitting. Superior stability towards photo-corrosion, appropriate band energy levels driving most photocatalytic reactions, and low-cost production are promising features of TiO₂. However, a primary limitation with TiO₂ is that it only absorbs ultraviolet light constituting less than 5% of the solar spectrum. In this work, we use a facile, low temperature, vacuum-free, and solution-route synthesis approach to rationally induce oxygen vacancy/Ti³⁺ defects to reduce the bandgap of TiO₂ to 2.0 eV (3.2 eV for pristine white TiO₂) to form brown TiO₂ with enhanced visible-light absorption. The mechanism of defect formation is systematically deduced from the detailed investigation through Raman spectroscopy, spin-sensitive technique, high-resolution microscopy, and surface analysis. The brown TiO₂ yielded 8.1 mmol h^?1g^?1_cat H₂ evolution without any cocatalyst under natural sunlight, which is a factor two higher than pristine (white) TiO₂. To the best of our knowledge, the observed H₂ evolution rate is the highest reported value under natural sunlight for any TiO₂-based photocatalyst. This work demonstrates the applicability of brown TiO₂ to fabricate large-area photocatalyst panels for the cost-effective production of solar H₂.     

Life cycle assessment of ethanol production in a rice-straw-based biorefinery in India

Clean Technologies and Environmental Policy - This work presents detailed life cycle assessment (LCA) of a novel process to produce ethanol from rice straw in India. The process has been...     

Pt-au alloying at the nanoscale

The formation of nanosized alloys between a pair of elements, which are largely immiscible in bulk, is examined in the archetypical case of Pt and Au. Element specific resonant high-energy X-ray diffraction experiments coupled to atomic pair distribution functions analysis and computer simulations prove the formation of Pt-Au alloys in particles less than 10 nm in size. In the alloys, Au-Au and Pt-Pt bond lengths differing in 0.1 ? are present leading to extra structural distortions as compared to pure Pt and Au particles. The alloys are found to be stable over a wide range of Pt-Au compositions and temperatures contrary to what current theory predicts. The alloy-type structure of Pt-Au nanoparticles comes along with a high catalytic activity for electrooxidation of methanol making an excellent example of the synergistic effect of alloying at the nanoscale on functional properties.     

Development and application of BioFeed model for optimization of herbaceous biomass feedstock production

An efficient and sustainable biomass feedstock production system is critical for the success of the biomass based energy sector. An integrated systems analysis framework to coordinate various feedstock production related activities is, therefore, highly desirable. This article presents research conducted towards the creation of such a framework. A breadth level mixed integer linear programming model, named BioFeed, is proposed that simulates different feedstock production operations such as harvesting, packing, storage, handling and transportation, with the objective of determining the optimal system level configuration on a regional basis. The decision variables include the design/planning as well as management level decisions. The model was applied to a case study of switchgrass production as an energy crop in southern Illinois. The results illustrated that the total cost varied between 45 and 49 $ Mg⁻¹ depending on the collection area and the sustainable biorefinery capacity was about 1.4 Gg d⁻¹. The transportation fleet consisted of 66 trucks and the average utilization of the fleet was 86%. On-farm covered storage of biomass was highly beneficial for the system. Lack of on-farm open storage and centralized storage reduced the system profit by 17% and 5%, respectively. Increase in the fraction of larger farms within the system reduced the cost and increased the biorefinery capacity, suggesting that co-operative farming is beneficial. The optimization of the harvesting schedule led to 30% increase in the total profit. Sensitivity analysis showed that the reduction in truck idling time as well as increase in baling throughput and output density significantly increased the profit.     

Design and evaluation of an actuated exoskeleton for examining motor control in stroke thumb

Chronic hand impairment is common following stroke. This paper presents an actuated thumb exoskeleton (ATX) to facilitate research in examining motor control and hand rehabilitation. The ATX presented in this work aims to provide independent bi-directional actuation in each of the 5 degrees of freedom (DOF) of the thumb using a novel flexible shaft-based mechanism that has 5 active DOF and 3 passive DOF. A prototype has been built and experiments have been conducted to measure the allowable workspace at the thumb and evaluate the kinematic and kinetic performance of the ATX. The experimental results show that the ATX is able to provide individual actuation at all five thumb joints with high joint velocity and torque capacities. Further improvement and future work are discussed.     

RF Characteristics of a high-performance, 10-fF//spl mu/m/sup 2/ capacitor in a deep trench

We report the properties of a novel polysilicon-insulator-polysilicon trench capacitor with a 380-/spl Aring/ Si/sub 3/N/sub 4/ dielectric that is designed and fabricated for high-frequency bypass and decoupling applications. The capacitor has a specific capacitance as high as 10 fF//spl mu/m/sup 2/ normalized to its footprint area, and a breakdown voltage greater than 15 V at room temperature. The measured S-parameters are in excellent agreement with simulations of an equivalent circuit model that includes a shunt substrate resistance to ground (R/sub sub/). A geometric factor /spl mu/ is defined as the ratio of the imaginary parts of Y/sub 11/ and -Y/sub 21/ at low frequency. The values of /spl mu/ and, consequently, R/sub sub/ are extracted from fitting the measured S-parameter data, and the layout dependence of /spl mu/ and R/sub sub/ is also explained by the model.