The use of confocal microscopy in evaluating corneal wound healing after excimer laser keratectomy

Corneal wound healing following excimer laser keratectomy is the major cause of regression of treatment results. The amount of anterior strorhal haze that develops may be influenced by topical medications. Over a period of 6 months, we followed 15 New Zealand white rabbit eyes that underwent excimer laser keratectomy with the VISX 193-nm ArF laser at a fluence of 150 mJ/cm² for a depth of 130 μm. Eyes were randomized to treatment with prednisolone acetate, diclofenac sodium (Voltaren), a combination of both, and a control group. Drops were administered four times a day for 1 week, two times a day for 3 weeks, and the drops were then tapered. All eyes were reepithelialized by 5 to 7 days. The tandem scanning confocal microscope (TSCM) was used to evaluate the corneal wound in vivo weekly for a month and monthly for 6 months. During the early postoperative period, the TSCM revealed significant anterior stromal keratocyte activation with cell elongation and the spindle-shaped appearance of fibroblasts in all groups. Collagenous stromal scarring was evident initially, then slowly decreased in all treatment groups. This study shows that TSCM is clinically useful for successive in vivo examinations of corneal wounds after excimer laser keratectomy and for comparing the effects of various topical medications.     

Characterization of an Ambient Coarse Particle Concentrator Used for Human Exposure Studies: Aerosol Size Distributions,Chemical Composition,and Concentration Enrichment

The goals of the experiments described herein involve determining in real time the size, concentration enrichment, and chemical composition of coarse-mode (<2.5 μm) and fine-mode (>2.5 μm) particles within the nonconcentrated and concentrated flows of a coarse particle concentrator used for human exposure studies. The coarse particle concentrator was intended to concentrate ambient particles in the PM_10–2.5 size range before sending them into a human exposure chamber. The aerodynamic size and chemical composition of particles in the upstream and downstream flows of the concentrator were monitored with an aerosol time-of-f1ight mass spectrometer (ATOFMS) for fixed time intervals over the course of three days. Based on the ATOFMS results, it was found that there was no change in the composition of the ten major particle types observed in the upstream and downstream flows of the concentrator under normal operating conditions. Furthermore, no new particle types were detected downstream that were not detected upstream of the concentrator. A characterization of the aerosol chemical composition and its dependence on sampling conditions is also discussed. Aerosol size distributions were measured with three aerodynamic particle-sizing (APS) instruments sampling simultaneously from different regions of the concentrator. The APS size distributions were used to scale ATOFMS data and measure the ambient concentration factors for the coarse particle concentrator and the exposure chamber. The average concentration factor (ratio of inlet number concentration to the outlet number concentration) for the particle concentrator was 60 + 17 for the 2.5–7.2 μm size range before dilution and transport to the exposure chamber. It was observed that not only were coarse particles being concentrated but fine (<2.5 μm) particles were being concentrated as well, with concentration factors ranging from 2–46 for aerodynamic particle sizes from 0.54–2.5 μm.     

Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein

The role of GRP78/BiP in coordinating endoplasmic reticular (ER) protein processing with mRNA translation was examined in GH3 pituitary cells. ADP-ribosylation of GRP78 and eukaryotic initiation factor (eIF)-2alpha phosphorylation were assessed, respectively, as indices of chaperone inactivation and the inhibition of translational initiation. Inhibition of protein processing by ER stress (ionomycin and dithiothreitol) resulted in GRP78 deribosylation and eIF-2 phosphorylation. Suppression of translation relative to ER protein processing (cycloheximide) produced approximately 50% ADP-ribosylation of GRP78 within 90 min without eIF-2 phosphorylation. ADP-ribosylation was reversed in 90 min by cycloheximide removal in a manner accelerated by ER stressors. Cycloheximide sharply reduced eIF-2 phosphorylation in response to ER stressors for about 30 min; sensitivity returned as GRP78 became increasingly ADP-ribosylated. Reduced sensitivity of eIF-2 to phosphorylation appeared to derive from the accumulation of free, unmodified chaperone as proteins completed processing without replacements. Prolonged (24 h) incubations with cycloheximide resulted in the selective loss of the ADP-ribosylated form of GRP78 and increased sensitivity of eIF-2 phosphorylation in response to ER stressors. Brefeldin A decreased ADP-ribosylation of GRP78 in parallel with increased eIF-2 phosphorylation. The cytoplasmic stressor, arsenite, which inhibits translational initiation through eIF-2 phosphorylation without affecting the ER, also produced ADP-ribosylation of GRP78.     

Integrated diamond networks for quantum nanophotonics

We demonstrate an integrated nanophotonic network in diamond, consisting of a ring resonator coupled to an optical waveguide with grating in- and outcouplers. Using a nitrogen-vacancy color center embedded inside the ring resonator as a source of photons, single photon generation and routing at room temperature is observed. Furthermore, we observe a large overall photon extraction efficiency (10%) and high quality factors of ring resonators (3200 for waveguide-coupled system and 12,600 for a bare ring).     

Static detection of application backdoors

Backdoors in legitimate software, whether maliciously inserted or carelessly introduced, are a risk that should be detected prior to the affected software or system being deployed. Automated static analysis of executable code can detect many classes of malicious behavior. This paper will cover the techniques that can be employed to detect special credentials, hidden commands, information leakage, rootkit behavior, anti-debugging, and time bombs.     

THE DEVELOPMENT OF LIQUID MALT*

The preservation of green malt by hot air drying is the most usual form of kilning. This not only ensures biological stability but also allows some flavour adjustment both by the evaporation of “green” flavours and the development of “biscuit” flavour on curing. A survey is now made of the development of a process of liquid kilning, in which starch conversion and extraction follows modification and precedes evaporative preservation and “green” flavour removal.     

Indoor hydrogen peroxide derived from ozone/d-limonene reactions

In this pilot study, performed in an office manipulated to resemble an environment with a strong indoor ozone source or a significant influx of outdoor air during a smog event, reactions between ozone and d-limonene produced hydroperoxides. Hydrogen peroxide (H202) presumably constituted the majority of the measured hydroperoxides, although a small amount of organic hydroperoxides (ROOH) may have contributed to the signal. Total hydroperoxides were 1.0-1.5 ppb at low air exchange rates (0.5-4 h(-1)) and 0.6-0.8 ppb at high air exchange rates (12-18 h-1). The net estimated yield ranged from 1.5 to 3.2%, consistent with values reported in the literature. Based on these yields and typical indoor scenarios, peak indoor concentrations of H202 are projected to be comparable with, but not significantly larger than, peak outdoor concentrations. Hygroscopic secondary organic aerosols (SOA; 10-100 microg m(-3)) were simultaneously generated by the ozone/d-limonene reactions; their co-occurrence with H202 provides a mechanism whereby H2O2 can be transported into the lower respiratory tract. The results demonstrate that reduced air exchange rates lead to increased concentrations of H2O2 and SOA as well as a shift in the size-distribution toward larger particles (0.3-0.7 microm diameter), potentially increasing the amount of H2O2 delivered to the lower respiratory region. This study increases our understanding of H2O2 exposures, including exposures to H2O2 associated with co-occurring hygroscopic aerosols. It also re-emphasizes the potential of ozone-driven chemistry to alter indoor environments, often producing products more irritating than their precursors.     

School children's use of computers and teachers' education in computer ergonomics

A national survey to investigate the education of teachers in computer-related ergonomics was carried out by postal questionnaire. The use of computers by primary school children (age 4-12 years) was also investigated. Data were collected from a random sample of 25% (n = 830) of primary schools in the Republic of Ireland. Questionnaires (n = 1863) were returned from 416 schools giving a response rate of 50.1%. Almost all schools (99.7%) had computers for children's use. The computers were most often (69.8%) used in the classroom. The majority (56.3%) of children worked in pairs. Most teachers (89.6%) had received computer training, but few (17.6%) had received ergonomics information during the training. Respondents were not satisfied with their current knowledge of ergonomics. Over 90% stated that they would like to receive further information by printed format or during a training course, rather than by computer (web or CD-ROM).