Thin natural gelatin/chitosan nanofibrous scaffolds for retinal pigment epithelium cells

Damage of the retinal pigmented epithelial cells causes various diseases such as age-related macular degeneration in retinal tissue. Nowadays, scientists are attempting to replace lost retinal cells with healthy and efficient cells that provide better conditions for recovering and preventing blindness. In this study, gelatin/chitosan nanofibrous scaffolds with mean diameters of 180?nm were fabricated for subretinal space through electrospinning. Thickness and morphology of the gelatin–chitosan scaffolds were analyzed by scanning electron microscopy (SEM). The results showed that the high rate of degradation, i.e., 90% damage was obtained after 1 month. The cell viability of gelatin/chitosan nanofibers were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The SEM results of cultured RPE on gelatin/chitosan scaffolds showed the appropriate adhesion of cells on the substrate. The results of the identity of RPE cells cultured on the scaffolds indicated that a large number of cells strongly expressed RPE65 and cytokeratin 8/18.     

Optimizing environmental flow regime by integrating river and reservoir ecosystems

Water Resources Management - The present study develops a novel form of optimization framework to assess environmental flow in the reservoirs in which upstream and downstream river ecosystems and...     

Nicotinic Acid Increases Adiponectin Secretion from Differentiated Bovine Preadipocytes through G-Protein Coupled Receptor Signaling

The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids in cattle. In mice the G-protein coupled receptor 109A (GPR109A) ligand NA positively affects the secretion of adiponectin, an important modulator of glucose and fat metabolism. In cattle, the corresponding data linking NA to adiponectin are missing. Our objective was to examine the effects of NA on adiponectin and AMPK protein abundance and the expression of mRNAs of related genes such as chemerin, an adipokine that enhances adiponectin secretion in vitro. Differentiated bovine adipocytes were incubated with pertussis toxin (PTX) to verify the involvement of GPR signaling, and treated with 10 or 15 µM NA for 12 or 24 h. NA increased adiponectin concentrations (p ≤ 0.001) and the mRNA abundances of GPR109A (p ≤ 0.05) and chemerin (p ≤ 0.01). Pre-incubation with PTX reduced the adiponectin response to NA (p ≤ 0.001). The NA-stimulated secretion of adiponectin and the mRNA expression of chemerin in the bovine adipocytes were suggestive of GPR signaling-dependent improved insulin sensitivity and/or adipocyte metabolism in dairy cows.     

Flow Behaviour of Modified 9Cr–1Mo Steel at Elevated Temperatures

In this investigation, the flow behaviour of modified 9Cr–1Mo steel at elevated temperatures is reported. To understand the flow behaviour of the steel, tensile tests were performed at nominal strain rate of 3 × 10^?3 s^?1 and temperatures in the range of 300–823 K. The yield strength and ultimate tensile strength were found to decrease with increase in temperature with a plateau in a intermediate temperature regime (523–673 K). Serrations were also observed in the tensile curve at intermediate temperatures which caused plateau/peak in tensile behaviour of material and was a typical manifestation of dynamic strain ageing. An attempt was made to represent the flow behaviour of the material using different constitutive equations viz., Hollomon, Ludwik, Swift, Ludwigson and Voce. It was observed that the Voce equation could describe the experimental flow curve at different temperatures quite well. Instantaneous work hardening rate with respect to flow stress exhibited two stages of hardening especially at relatively lower temperatures.     

Radiation dose from dissolved radon in potable waters of the Bangalore environment,South India

Potable waters from various locations of the Bangalore environment were investigated for their ²²²Rn concentrations by the emanometry method. About 94 groundwater (borewell) samples were analysed for ²²²Rn concentrations and found to vary in the range 5.3–283.4 Bq L^?1 with a mean value of 87 Bq L^?1. Frequency distribution showed the ²²²Rn concentration in a large number of samples in the range of 0–50 Bq L^?1. From the measured concentrations, the effective doses (lung and stomach) for the population of the region were estimated. The effective dose was found to vary from 42.6 to 2280.2 µSv y^?1 with a mean value 702.5 µSv y^?1. The effect of boiling of water showed a drastic reduction in the ²²²Rn concentration. The results of the present investigation are systematically analysed, compared with the literature values and discussed.     

Class of majority-logic-decodable codes with a minimum distance of 4

We prove that the (n = 4a2 + 4a + l, k = 4a2) binary code generated by 1+X2a+X2a+1+X4a+1 has a minimum distance of 4 and is 1-step majority-Iogic-decodable.     

Optimal Design of Concrete Canal Section for Minimizing Costs of Water Loss,Lining and Earthworks

Canal section design with minimum cost, which can be considered as an objective function, involves minimization of total costs per unit length of the canal, including direct costs of per cubic meter earthworks and per meter canal lining and indirect costs of water losses through canal seepage and evaporation. Since the costs (both direct and indirect) are associated with the canal geometry and dimensions, it is possible to lower them by optimization of the mentioned objective function. For this purpose, some constraints were subjected and considered to solve the problem. Flow discharge, as the main constraint, was considered in addition to the minimum permissible velocity and Froude’s number, as subsidiary constraints. MATLAB programming software was used to demonstrate and run the optimization algorithm. The results finally were illustrated in forms of dimensionless graphs, which simplify the optimum design of canal dimensions with minimum cost per meter length. Comparing the results with other similar studies, however show the importance and role of earthworks and lining costs, as well as including the subsidiary constraints in the optimization process.     

NOx formation and selective non-catalytic reduction (SNCR) in a fluidized bed combustor of biomass

Caledonian Paper (CaPa) is a major paper mill, located in Ayr, Scotland. For its steam supply, it previously relied on the use of a Circulating Fluidized Bed Combustor (CFBC) of 58 MW_th, burning coal, wood bark and wastewater treatment sludge.It currently uses a bubbling fluidized bed combustor (BFBC) of 102 MW_th to generate steam at 99 bar, superheated to 465 °C. The boiler is followed by steam turbines and a 15 kg/s steam circuit into the mill. Whereas previously coal, wood bark and wastewater treatment sludge were used as fuel, currently only plantation wood (mainly spruce), demolition wood, wood bark and sludge are used.Since these biosolids contain nitrogen, fuel NO_x is formed at the combustion temperature of 850–900 °C. NO_x emissions (NO + NO₂) vary on average between 300 and 600 mg/Nm³ (dry gas). The current emission standard is 350 mg/Nm³ but will be reduced in the future to a maximum of 233 mg/Nm³ for stand-alone biomass combustors of capacity between 50 and 300 MW_th according to the EU LCP standards. NO_x abatement is therefore necessary.In the present paper we firstly review the NO_x formation mechanisms, proving that for applications of fluidized bed combustion, fuel NO_x is the main consideration, and the contribution of thermal NO_x to the emissions insignificant.We then assess the deNO_x techniques presented in the literature, with an updated review and special focus upon the techniques that are applicable at CaPa. From these techniques, Selective Non-catalytic Reduction (SNCR) using ammonia or urea emerges as the most appropriate NO_x abatement solution.Although SNCR deNO_x is a selective reduction, the reactions of NO_x reduction by NH₃ in the presence of oxygen, and the oxidation of NH₃ proceed competitively.Both reactions were therefore studied in a lab-scale reactor and the results were transformed into design equations starting from the respective reaction kinetics. An overall deNO_x yield can then be predicted for any operating temperature and NH₃/NO_x ratio.We then present data from large-scale SNCR-experiments at the CFBC of CaPa and compare results with the lab-scale model predictions, leading to recommendations for design and operation. Finally the economic impact is assessed of implementing SNCR-technology when applying an NH₃ SNCR or urea SNCR to the CFBC at CaPa.