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101.
Yutaka Furutani Mariko Toguchi Shoko Higuchi Kaori Yanaka Luc Gailhouste Xian-Yang Qin Takahiro Masaki Sae Ochi Tomokazu Matsuura 《International journal of molecular sciences》2021,22(21)
Inhaled nebulized interferon (IFN)-α and IFN-β have been shown to be effective in the management of coronavirus disease 2019 (COVID-19). We aimed to construct a virus-free rapid detection system for high-throughput screening of IFN-like compounds that induce viral RNA degradation and suppress the replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We prepared a SARS-CoV-2 subreplicon RNA expression vector which contained the SARS-CoV-2 5′-UTR, the partial sequence of ORF1a, luciferase, nucleocapsid, ORF10, and 3′-UTR under the control of the cytomegalovirus promoter. The expression vector was transfected into Calu-3 cells and treated with IFN-α and the IFNAR2 agonist CDM-3008 (RO8191) for 3 days. SARS-CoV-2 subreplicon RNA degradation was subsequently evaluated based on luciferase levels. IFN-α and CDM-3008 suppressed SARS-CoV-2 subreplicon RNA in a dose-dependent manner, with IC50 values of 193 IU/mL and 2.54 μM, respectively. HeLa cells stably expressing SARS-CoV-2 subreplicon RNA were prepared and treated with the IFN-α and pan-JAK inhibitor Pyridone 6 or siRNA-targeting ISG20. IFN-α activity was canceled with Pyridone 6. The knockdown of ISG20 partially canceled IFN-α activity. Collectively, we constructed a virus-free rapid detection system to measure SARS-CoV-2 RNA suppression. Our data suggest that the SARS-CoV-2 subreplicon RNA was degraded by IFN-α-induced ISG20 exonuclease activity. 相似文献
102.
Shoko Komiyama Yuji Sutou Junichi Koike 《Metallurgical and Materials Transactions A》2011,42(11):3310-3315
Effects of nitrogen content on the microstructure, hardness, and friction coefficient of Ti-Mo-N coating films were investigated.
Ti-Mo-N films were deposited onto an AISI304 stainless steel substrate by reactive r.f. sputtering in the mixture of argon
and nitrogen gases with various gas flow rates. The hardness and friction coefficients were measured by nanoindentation and
ball-on-disk testing systems, respectively. The hardness of the Ti-Mo-N films increased with increasing a nitrogen gas flow
rate
( f\textN2 ) \left( {f_{{{\text{N}}_{2} }} } \right) and showed a maximum hardness of about 30 GPa at a
f\textN2 = 0.3 \textccm f_{{{\text{N}}_{2} }} = 0.3\,{\text{ccm}} . On the one hand, the films deposited at
f\textN2 3 1.0 \textccm f_{{{\text{N}}_{2} }} \ge 1.0\;{\text{ccm}} showed a constant hardness value of approximately 25 GPa. On the other hand, the friction coefficient of the Ti-Mo-N film
decreased with increasing N content and was 0.44 in the film deposited at
f\textN2 = 2.0 \textccm. f_{{{\text{N}}_{2} }} = 2.0\;{\text{ccm}}. 相似文献
103.
Ferroelectric and Photostrictive Properties of Fine-Grained PLZT Ceramics Derived from Mechanical Alloying 总被引:2,自引:1,他引:1
Kenta Takagi Shoko Kikuchi Jing-Feng Li Hiroshi Okamura Ryuzo Watanabe Akira Kawasaki 《Journal of the American Ceramic Society》2004,87(8):1477-1482
Single-phase lead lanthanum zirconate titanate (PLZT) solid solution powder was synthesized from the constituent oxides at ambient temperature through a mechanical alloying (MA) process and was then densified to fine-grained ceramics by sintering and hot-pressing. The anomalous photovoltaic effect (APV) and photoinduced strain of the resultant PLZT ceramics were investigated and analyzed in association with the influence of grain size. It was found that a photoinduced voltage up to 6000 V·cm−1 can be obtained as the grain size is reduced to 0.42 μm. This is extremely high and about three times that achievable in normal micrometer-grained PLZT ceramics. The maximum photoinduced strain of the PLZT ceramics with an average grain diameter of 0.54 μm reached 0.01%, which is equivalent to electric-field-induced strain of common piezoelectric materials. 相似文献
104.
Formation and Microstructures of Whipped Oils Composed of Vegetable Oils and High-Melting Fat Crystals
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Shoko Mishima Atsushi Suzuki Kiyotaka Sato Satoru Ueno 《Journal of the American Oil Chemists' Society》2016,93(11):1453-1466
This paper reports the experimental results of processes used for the formation of whipped oils composed of vegetable oils (salad oil) and high‐melting fat crystals [fully hydrogenated rapeseed oil rich in behenic acid (FHR‐B)]. No emulsifier was added to form this whipped oil. Microprobe FT‐IR spectroscopy, synchrotron radiation microbeam X‐ray diffraction (SR‐μ‐XRD), polarized optical microscopy, and differential scanning calorimetry (DSC) were employed to observe fine fat crystal particles of the most stable polymorph of β (β‐fat crystal), FHR‐B, and their adsorption at the air–oil surface before, during, and after the formation of the whipped oil. The results obtained revealed the following: (1) The preparation of an organogel composed of salad oil and small fibrous β‐fat crystals using a special tempering procedure was a prerequisite for forming whipped oil. (2) The β‐fat crystals were adsorbed at the air–oil surface to encapsulate the air bubbles during the formation process of whipped oil. (3) The values of overrun of the whipped oil reached >200 % after an aeration time of 30 min at 20 °C. (4) The SR‐μ‐XRD experiments demonstrated that the lamellar planes of the β‐fat crystals near the air–oil surface were arranged almost parallel to the air–oil surface plane. The present study provides the first evidence that tiny fat crystal particles may cause aeration in liquid oils without the addition of other whip‐assisting substances such as emulsifier crystals. 相似文献
105.
Torbjörn Eriksson Shoko YamadaPrasanna Venkatesh Krishnan Sankar RamasamyBabak Heidari 《Microelectronic Engineering》2011,88(3):293-299
Nanoimprint lithography (NIL) has developed from an emerging nanoreplication technology into a mature and industrially viable manufacturing technology. It is now by far the fastest and most cost-efficient nanoreplication method available. This technology has been successfully implemented in the manufacturing of High Brightness LED’s. However, manufacturing of HBLED’s in high volume using nanoimprint lithography faces a few particular challenges. Perhaps the most significant is to produce perfectly imprinted nanostructures on the epitaxially grown substrates with high quality and yield. This is especially important since the substrates are expensive and since the imprint step is close to the end of the production process. This means that the value of the processed substrates is very high. In this work, 8000 imprints were produced. Measured data from the imprinted substrates shows consistent results. It is also shown that the 8000 imprints have been performed using the same stamp without significant degradation. After 8000 imprints, the yield from the nanoimprint lithography step is 99.15%. 相似文献
106.
Huijuan Jia Kenji Saito Wanping Aw Shoko Takahashi Manaka Hanate Yukio Hasebe Hisanori Kato 《Journal of Functional Foods》2013,5(4):1611-1619
Egg shell membrane (ESM) is a natural and safe food by-product from egg processing whilst there is little information about the role of ESM as a food component. Effects of dietary ESM on gene expression in rat liver were investigated through DNA microarray comprehensive analysis. The expression of smooth muscle-α-actin, which is a marker of hepatic stellate cells (HSCs) activation, and integrin beta-like 1, decorin, asporin, lumican and collagen type 1 alpha 1, which are components of extracellular matrix (ECM) and involved in the up-regulation of HSCs activation and fibrosis, was found to be significantly down-regulated after 14 days of ESM treatment. Subsequently, serum obtained from rats given ESM diet also suppressed the expression of COL1A1 and COL1A2 in human hepatoma C3A cells. Our in vivo and ex vivo findings demonstrate that these gene alterations may contribute to the beneficial effect of ESM partially through down-regulation of c-jun and c-fos signal transduction thereby blunting HSCs activation and eventually preventing liver fibrosis. These outcomes not only provide novel information about the functional and nutritional availability of ESM, but also might contribute to the field of environmental protection. 相似文献
107.
Takaya Sato Shoko MarukaneTakashi Morinaga Taichi UemuraKunihiro Fukumoto Satoshi Yamazaki 《Journal of power sources》2011,196(5):2835-2840
We present a new method to improve the rate capability of an electric double layer capacitor (EDLC) using a thin polymer layer having a high concentration of carbon material on a current collector (CLC). A novel thermocuring coating composed of a glycol-chitosan, a pyromellitic acid and a conductive carbon powder can form stable CLC on a metal foil current collector simply by spreading and curing at 160 °C for a couple of minutes. We compared the performance of some demonstration EDLC cells using three kinds of current collector: a conventional aluminum oxide foil for EDLC, an aluminum foil and an aluminum foil with CLC. The cell with the CLC had a much higher rate capability than the cell without CLC. Only the CLC cell was able to discharge at a current density of 500C. This cell shows a slight deterioration in capacity in a high temperature, continuous charging, life test, and the CLC has a suppressing effect on the internal resistance increase of EDLCs. The use of a CLC film current collector is one of the most effective and simple methods for the improvement of EDLC rate performance. In particular, a current collector consisting of aluminum foil coupled with a CLC promises to be a low cost alternative to the aluminum oxide foil commonly used in EDLCs. 相似文献
108.
109.
A new system to obtain ultrathin self-standing polyelectrolyte multilayer membranes at physiological conditions is introduced. On the surface of a substrate, a hybrid film structure composed of two compartments, (1) a pH-responsive film segment formed via hydrogen bonds and (2) a polyelectrolyte multilayer film on top of 1, was assembled. The pH-responsive polymer multilayer segments disintegrate at a neutral pH and release self-standing polyelectrolyte multilayer films. The obtained self-supporting polyelectrolyte multilayer membranes had thicknesses of 55 to several hundred nanometers and areas of a few square centimeters, approximately. The preparation method introduced here avoids harsh release conditions and thus broadens the choice of materials that can be incorporated into the self-standing film. 相似文献
110.
In this review article, we compare the performance of two computing systems: quantum computing and coherent computing. A layered architecture for circuit-model quantum computing, employing surface code quantum error correction, has been recently discussed. Using this concrete hardware platform, it is possible to provide resource analysis for executing the fault-tolerent quantum computing for prime number factoring and molecular eigen-energy calculation that cannot be solved by the present day computing systems. A particular quantum computing system could solve such problems on the time scale of 1-10 days by using 108 – 109 physical qubits. We discuss an alternative computing system based on an injection-locked laser network wnicn is called a coherent computing system here. A three-dimensional Ising model is mapped onto the mutually injection-locked slave laser network, while the independent injection signal from a master laser implements a Zeeman Hamiltonian. In this computing system, an Ising spin taking either up or down state is represented by the polarization degrees of freedom, right or left circular polarizations, of the lasing photons in each slave laser. A spin-spin coupling coefficient is implemented by simple linear polarization optics connecting the two slave lasers. We numerically study the scaling law of the proposed machine against the anti-ferromagnetic Ising model with varying problem size M. A transient time to reach a steady state polarization configuration is inversely proportional to the locking bandwidth and does not depend on the problem size strongly up to M=1000. 相似文献