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21.
Tsuda T Nemoto N Kawakami K Mochizuki E Kishida S Tajiri T Kushibiki T Kuwabata S 《Chembiochem : a European journal of chemical biology》2011,12(17):2547-2550
A facile pretreatment process for SEM: The use of room temperature ionic liquids (RTILs) provides an interesting method for SEM of biological specimens. We used a novel and concise method of pretreatment, excluding fixation or Au sputtering steps. Fine and smooth-textured SEM images of a wide variety of biological specimens treated in this way were observed without artefacts. 相似文献
22.
Tatsunari Ohkubo Takaaki Shiina Kayoko Kawaguchi Daisuke Sasaki Rena Inamasu Yue Yang Zhuoqi Li Keizaburo Taninaka Masaki Sakaguchi Shoko Fujimura Hiroshi Sekiguchi Masahiro Kuramochi Tatsuya Arai Sakae Tsuda Yuji C. Sasaki Kazuhiro Mio 《International journal of molecular sciences》2022,23(23)
Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented. 相似文献
23.
Dongfang Li Shoko Onodera Shu Deng Bashaer Alnujaydi Qing Yu Jing Zhou 《International journal of molecular sciences》2022,23(22)
Intermittent fasting confers protections to various diseases including autoimmune disorders, but the specific effects of intermittent fasting on Sjögren’s syndrome (SS) remains inconclusive. The present study was undertaken to determine the specific impact of alternate-day fasting (ADF) on newly established SS-like sialadenitis using non-obese diabetic (NOD) mice. Female NOD mice were deprived of food every other day from 10 to 13 weeks of age, the early stage of established SS, and then analyzed for the disease characteristics. Mice in the ADF group had higher salivary flow rate and attenuated submandibular gland (SMG) inflammation, compared to the control mice fed with standard chow ad libitum. The improvements were accompanied with a decrease in the total leukocytes, T and B lymphocytes and activated CD4 and CD8 T cells, and a down-regulation of pro-inflammatory cytokines IFN-γ and IL-17, chemokine receptor CXCR3 and its ligands CXCL9 and CXCL11 in the SMGs. ADF also led to elevated mRNA levels of water channel protein aquaporin 5 and tight junction protein claudin-1, two factors crucial for normal salivary secretion in the SMGs. In addition, ADF reduced the proportion of IFN-γ- and IL-17- expressing CD4 T cells and diminished mRNA levels of IFN-γ, TNF-α, and IL-17 in the total submandibular draining lymph node cells. Taken together, ADF is effective in ameliorating newly established SS-associated salivary gland exocrinopathy in NOD mice. 相似文献
24.
Effect of Compositional Variations in the Lead Lanthanum Zirconate Stannate Titanate System on Electrical Properties 总被引:2,自引:0,他引:2
Kelley Markowski Seung-Eek Park Shoko Yoshikawa L. Eric Cross 《Journal of the American Ceramic Society》1996,79(12):3297-3304
The purpose of this work was to evaluate the effect of compositional modifications on the electrical properties of lead lanthanum zirconate stannate titanate (PLZST) ceramics, as well as to examine their electrically induced phasechange behavior. Variations in the Ti:Sn ratio were evaluated. Increased Ti4+ content produced the following: decreased switching field, related to an increased antiferro-electric-ferroelectric (AFE-FE) transition temperature; constant hysteresis (Δ E ) correlated with a constant temperature of the maximum dielectric constant (Tmax ); a sharper dielectric-constant maximum peak; and increased room-temperature dielectric constant ( K ). Variations in the Zr:Sn ratio also were evaluated. Increased Zr4+ content produced the following: increased hysteresis with increased Tmax , decreased maximum dielectric constant, and decreased switching field with increased AFE-FE transition temperature (TAFE _FE ). From these results, with respect to compositional modifications, the AFE-FE switching field (EAFE _FE ) and Δ E were observed to be dependent strongly on TAFE _FE and Tmax , respectively. Negligible change existed in the strain achievable at the switching field, which remained constant for all compositions at ∼0.16%. The significance of this research was the ability demonstrated to tailor the properties of phase-change materials through compositional modifications. 相似文献
25.
Shoko Hira Akiko Matsumoto Ken Kihara Sakuichi Ohtsuka 《Journal of the Society for Information Display》2019,27(7):409-426
The mutual understanding of color‐normal observers (CNOs) and color‐defective observers (CDOs) is now essential because personal color information display environments have been widely adopted. However, existing tools for CDOs offer only color discrimination; they fail to support color impression (ie, saturation and contrast). Therefore, we need a novel tool that offers help in distinguishing opponent colors, while preserving color saturation. We introduce two key techniques for realizing this difficult goal. The former is the repeated sequential display of the original and processed images to support the formation of unified correct percepts that provide discrimination of both red‐green and yellow‐blue opponent colors. One image, ie, original, exhibits correct yellow‐blue but distorted red‐green information for CDOs while the other, ie, processed, provides synthesized distinguishable red‐green but confusable yellow‐blue information for CDOs; here, hue rotation (HR) is useful for advanced users whereas hue blending (HB) is suitable for general. The latter is realized by the real‐time video processing available on smartphones; our algorithms support direct processing of the digital component video signal formats (eg, Y, CR, and CB). Subjective tests suggest that the two above‐mentioned algorithms will, along with embedding a lightweight real‐time dichromatic simulation facility for CNOs, greatly help the mutual understanding of CNOs and CDOs. 相似文献
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Dr. Hiroyuki Shinchi Tomoya Nakamura Hayato Ota Prof. Shoko Nishihara Dr. Masahiro Wakao Prof. Yasuo Suda 《Chembiochem : a European journal of chemical biology》2019,20(14):1810-1816
Developing methods to determine cell type and cell state has been a significant challenge in the field of cancer diagnosis as well as in typing and quality verification for cultured cells. Herein, we report a cell profiling method based on binding interactions between cell-surface sugar-chain-binding proteins and sugar-chain-immobilized fluorescent nanoparticles (SFNPs), together with a method for cell typing and cell quality verification. Binding profiles of cells against sugar chains were analyzed by performing flow cytometry analysis with SFNPs. Discrimination analysis based on binding profiles could classify cell type and evaluate the quality of cultured cells. By applying our method to differentiated cells originating from conventional cell lines and also to mouse embryotic stem cells, we could detect the cells before and after differentiation. Our method can be utilized not only for the biofunctional analysis of cells but also for diagnosis of cancer cells and quality verification of cultured cells. 相似文献
29.
Yu Mitsui Shoko Seto Mari Nishio Kazuya Minato Kimiharu Ishizawa Shigeru Satoh 《Biomass & bioenergy》2010,34(4):467-473
The present study was conducted to select willow (Salix spp.) clones with a high potential for use as biomass energy crops in the southern region of Tohoku district in Japan. Cuttings of 8 willow clones were planted on an abandoned farmland near Sendai (av. annual temp., 10.9 °C) in March 2006, grown throughout the year and cut back in late December 2006 to resprout from the remaining stools in March 2007. The biomass yield in December 2007, after the first growing season, was highest in Salix pet-susu clone KKD, followed by Salix pseudolinearis clone FXM and Salix sachalinensis clone SEN. The biomass yield on December 2008, after the second growing season, was again highest in clone KKD followed by clone FXM, S. pet-susu clone HB471 and S. sachalinensis clone SEN; the average annual yield of dry mass after the second growing season being 3.09, 2.58, 2.17 and 1.85 kgDM plant?1 for the clones in this order. Plant growth form differed among the clones. Clones FXM and SEN had several shoots of almost uniform base diameter, whereas clones KKD and HB471 showed plagiotropic growth with one thick and several thin shoots. The calorific values of dried stem segments were similar among clones, ranging from 18.7 to 19.1 kJ g?1. The dried stem segments contained 78.9–81.2 wt.% hollocellulose, 27.2–32.3 wt.% lignin and 2.1–4.0 wt.% extractives with ethanol-benzene, depending on clones. Based on these results, we could select four clones (KKD, FXM, HB471 and SEN) suitable for biomass production by SRWC in this area. 相似文献
30.
Yosuke Matsuo Yusuke Fujita Sachiko Ohnishi Takashi Tanaka Hideaki Hirabaru Takanori Kai Hiroshi Sakaida Shoko Nishizono Isao Kouno 《Food chemistry》2010
Chemical constituents of the leaves of rabbiteye blueberry (Vaccinium ashei READE) were investigated in detail. The major phenolic components were caffeoyl quinic acids, flavonol glycosides, flavan-3-ols and proanthocyanidins. Catechins and proanthocyanidins having additional phenylpropanoid units, such as cinchonains, kandelins and mururins, characterised the polyphenols of this plant. Among them, vaccinin A, an isomer of mururin A, was found to be a new compound, and the structure was characterised by spectroscopic methods. The most abundant polyphenols (11.3% of freeze-dried leaves) were oligomeric proanthocyanidins. Thiol degradation with mercaptoethanol indicated that the polymer was constituted of (+)-catechin and (−)-epicatechin as the terminal units and (−)-epicatechin, procyanidin A-2, and cinchonains Ia and Ib as the extension units. Mass spectral analysis suggested the presence of at least dodecamers with A-type linkages and phenylpropanoid moieties. 相似文献