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排序方式: 共有123条查询结果,搜索用时 31 毫秒
91.
Takahiro Takada Sea Fue Wang Shoko Yoshikawa Sei-Joo Jang Robert E. Newnham 《Journal of the American Ceramic Society》1994,77(9):2485-2488
The effects of glass additions on the properties of (Zr,Sn)TiO4 as a microwave dielectric material were investigated. The (Zr,Sn)TiO4 ceramics with no glass addition sintered at 1360°C gave Q = 4900 and K = 37 at 7.9 GHz . Several glasses, including SiO2 , B2 O3 , 5ZnO–2B2 O3 , and nine commercial glasses, were tested during this study. Among these glasses, (Zr,Sn)TiO4 sintered with ZnO-B2 O3 –SiO2 (Corning 7574) showed more than 20% higher density than that of pure (Zr,Sn)TiO4 sintered at the same temperature. A 5-wt% addition of SiO2 , to (Zr,Sn)TiO4 , when sintered at 1200°C, gave the best Q : Q = 2700 at 9 GHz. Results of XRD analysis and scanning electron microscopy and the effect of glass content are also presented. 相似文献
92.
Antoine Pringuet Takuma Takahashi Shoko Baba Yuta Kamo Zenji Kato Keizo Uematsu Satoshi Tanaka 《Journal of the American Ceramic Society》2016,99(10):3217-3219
Grain‐oriented polycrystalline alumina (PCA) ceramics with very high, nearly single‐crystal transparency were successfully fabricated through colloidal processing in a high magnetic field followed by hot isostatic pressing. The c‐axis of the produced material was oriented along the direction parallel to the magnetic field. The transmittance of the specimen with a thickness of 0.8 mm was 78% at a wavelength of 650 nm, close to the reported value of 84.5% for sapphire and more than 10% higher than the largest transmittance magnitude ever reported for PCA ceramics. In addition, the Na salt of 4,5‐dihydroxy‐1,3‐benzenedisulfonic acid (named “Tiron”) was found to be a highly effective dispersant for processing high‐purity materials. 相似文献
93.
Sunao Sugita Ayumi Hono Shoko Fujino Yoko Futatsugi Yuta Yunomae Norio Shimizu Masayo Takahashi 《International journal of molecular sciences》2021,22(22)
Contamination of cells/tissues by infectious pathogens (e.g., fungi, viruses, or bacteria, including mycoplasma) is a major problem in cell-based transplantation. In this study, we tested a polymerase chain reaction (PCR) method to provide rapid, simple, and sensitive detection of mycoplasma contamination in laboratory cultures for clinical use. This mycoplasma PCR system covers the Mycoplasma species (spp.) listed for testing in the 17th revision of the Japanese Pharmacopoeia, and we designed it for use in transplantable retinal cells. Here, we analyzed mycoplasma contamination in induced pluripotent stem cell (iPS cell)-derived transplantable retinal pigment epithelium (RPE) cells. In the spike tests to RPE cells with nine species of class Mollicutes bacteria, including seven Mycoplasma spp. and one of each Acholeplasma spp. and Ureaplasma spp., contamination at the concentration of 100 and 10 CFU/mL were detected with 100% probability in all cases, while 1 CFU/mL had a detection rate of 0–75%. DNA prepared from bacteria species other than class Mollicutes species was not detectable, indicating the specificity of this PCR. While iPS cells and iPS-RPE cells established in our laboratory were all negative by this PCR, some of the commercially available cell lines were positive. Cells for transplantation should never have infection, as once pathogens are implanted into the eyes, they can cause severe intraocular inflammation. Thus, it is imperative to monitor for infections in the transplants, although generally, mycoplasma infection is difficult to detect. 相似文献
94.
Takahiro Takada Sea Fue Wang Shoko Yoshikawa Sei-Joo Jang Robert E. Newnham 《Journal of the American Ceramic Society》1994,77(7):1909-1916
The effect of glass addition on the properties of BaO–TiO2 -WO3 microwave dielectric material N-35, which has Q = 5900 and K = 35 at 7.2 GHz for samples sintered at 1360°C, was investigated. Several glasses including B2 O3 , SiO2 , 5ZnO–2B2 O3 , and nine other commercial glasses were selected for this study. Among these glasses, one with a 5 wt% addition of B2 O3 to N-35, when sintered at 1200°C, had the best dielectric properties: Q = 8300 and K = 34 at 8.5 GHz. Both Q and K increased with firing temperature as well as with density. The Q of N-35, when sintered with a ZnO–B2 O3 glass system, showed a sudden drop in the sintering temperature to about 1000°C. The results of XRD, thermal analysis, and scanning electron microscopy indicated that the chemical reaction between the dielectric ceramics and glass had a greater effect on Q than on the density. The effects of the glass content and the mixing process on the densification and microwave dielectric properties are also presented. Ball milling improved the densification and dielectric properties of the N-35 sintered with ZnO–B2 O3 . 相似文献
95.
Shoko Fujimura Kazuhiro Mio Tatsunari Ohkubo Tatsuya Arai Masahiro Kuramochi Hiroshi Sekiguchi Yuji C. Sasaki 《International journal of molecular sciences》2022,23(4)
Membrane proteins change their conformations in response to chemical and physical stimuli and transmit extracellular signals inside cells. Several approaches have been developed for solving the structures of proteins. However, few techniques can monitor real-time protein dynamics. The diffracted X-ray tracking method (DXT) is an X-ray-based single-molecule technique that monitors the internal motion of biomolecules in an aqueous solution. DXT analyzes trajectories of Laue spots generated from the attached gold nanocrystals with a two-dimensional axis by tilting (θ) and twisting (χ). Furthermore, high-intensity X-rays from synchrotron radiation facilities enable measurements with microsecond-timescale and picometer-spatial-scale intramolecular information. The technique has been applied to various membrane proteins due to its superior spatiotemporal resolution. In this review, we introduce basic principles of DXT, reviewing its recent and extended applications to membrane proteins and living cells, respectively. 相似文献
96.
97.
Chitinases belonging to the GH19 family have diverse loop structure arrangements. A GH19 chitinase from rye seeds (RSC-c) has a full set of (six) loop structures that form an extended binding cleft from -4 to +4 (“loopful”), while that from moss (BcChi-A) lacks several loops and forms a shortened binding cleft from -2 to +2 (“loopless”). We herein inserted a loop involved in sugar residue binding at subsites +3 and +4 of RSC-c (Loop-II) into BcChi-A (BcChi-A+L-II), and the thermal stability and enzymatic activity of BcChi-A+L-II were then characterized and compared with those of BcChi-A. The transition temperature of thermal unfolding decreased from 77.2 ˚C (BcChi-A) to 63.3 ˚C (BcChi-A+L-II) by insertion of Loop-II. Enzymatic activities toward the chitin tetramer (GlcNAc)4 and the polymeric substrate glycol chitin were also suppressed by the Loop-II insertion to 12 and 9 %, respectively. The Loop-II inserted into BcChi-A was found to be markedly flexible and disadvantageous for protein stability and enzymatic activity. 相似文献
98.
99.
Afaf Eladl Yudai Yamaoki Shoko Hoshina Haruka Horinouchi Keiko Kondo Shou Waga Takashi Nagata Masato Katahira 《International journal of molecular sciences》2021,22(7)
Origin recognition complex (ORC) binds to replication origins in eukaryotic DNAs and plays an important role in replication. Although yeast ORC is known to sequence-specifically bind to a replication origin, how human ORC recognizes a replication origin remains unknown. Previous genome-wide studies revealed that guanine (G)-rich sequences, potentially forming G-quadruplex (G4) structures, are present in most replication origins in human cells. We previously suggested that the region comprising residues 413–511 of human ORC subunit 1, hORC1413–511, binds preferentially to G-rich DNAs, which form a G4 structure in the absence of hORC1413–511. Here, we investigated the interaction of hORC1413-511 with various G-rich DNAs derived from human c-myc promoter and telomere regions. Fluorescence anisotropy revealed that hORC1413–511 binds preferentially to DNAs that have G4 structures over ones having double-stranded structures. Importantly, circular dichroism (CD) and nuclear magnetic resonance (NMR) showed that those G-rich DNAs retain the G4 structures even after binding with hORC1413–511. NMR chemical shift perturbation analyses revealed that the external G-tetrad planes of the G4 structures are the primary binding sites for hORC1413–511. The present study suggests that human ORC1 may recognize replication origins through the G4 structure. 相似文献
100.
Shoko Komiyama Yuji Sutou Junichi Koike 《Metallurgical and Materials Transactions A》2011,42(11):3310-3315
Effects of nitrogen content on the microstructure, hardness, and friction coefficient of Ti-Mo-N coating films were investigated.
Ti-Mo-N films were deposited onto an AISI304 stainless steel substrate by reactive r.f. sputtering in the mixture of argon
and nitrogen gases with various gas flow rates. The hardness and friction coefficients were measured by nanoindentation and
ball-on-disk testing systems, respectively. The hardness of the Ti-Mo-N films increased with increasing a nitrogen gas flow
rate
( f\textN2 ) \left( {f_{{{\text{N}}_{2} }} } \right) and showed a maximum hardness of about 30 GPa at a
f\textN2 = 0.3 \textccm f_{{{\text{N}}_{2} }} = 0.3\,{\text{ccm}} . On the one hand, the films deposited at
f\textN2 3 1.0 \textccm f_{{{\text{N}}_{2} }} \ge 1.0\;{\text{ccm}} showed a constant hardness value of approximately 25 GPa. On the other hand, the friction coefficient of the Ti-Mo-N film
decreased with increasing N content and was 0.44 in the film deposited at
f\textN2 = 2.0 \textccm. f_{{{\text{N}}_{2} }} = 2.0\;{\text{ccm}}. 相似文献