Serum from diabetic BB/W rats enhances calcium currents in primary sensory neurons

We examined the hypothesis that exposure of nondiabetic rat dorsal root ganglion (DRG) neurons to sera from diabetic BB/W rats would produce an increase in calcium currents associated with impaired regulation of the inhibitory G protein-calcium channel complex. Acutely dissociated rat DRGs were incubated for 18-24 h in medium supplemented with sera (10% vol/vol) from either diabetic rats with neuropathy or age-matched, nondiabetic controls. Exposure of DRG neurons to sera from diabetic BB/W rats resulted in a surface membrane immunofluorescence pattern when treated with an anti-rat light-chain antibody that was not observed in neurons exposed to control sera. Calcium current density (IDCa) was assessed with the use of the whole cell variation of the patch-clamp technique. IDCa in neurons exposed to diabetic sera was significantly increased compared with neurons exposed to control sera. Guanine nucleotide-binding (G) protein regulation of calcium channel function was examined with the use of a two-pulse "facilitation" or IDCa enhancement protocol in the presence of activators [guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S)] or antagonists [guanosine 5'-O-(2-thiodiphosphate) (GDP beta S) and pertussis toxin (PTX)] of G protein function. Facilitation was significantly decreased in neurons exposed to diabetic sera. Intracellular diffusion of neurons with GDP beta s blocked facilitation, whereas dialysis with GTP gamma s increased facilitation to a similar magnitude in neurons exposed to either diabetic or control sera. Treatment with PTX resulted in a significant increase in IDCa and approximately 50% decrease in facilitation in neurons treated with control sera but no significant changes in neurons exposed to diabetic sera. We conclude that serum from diabetic BB/W rats with neuropathy contains an autoimmune immunoglobulin that impairs regulation of the inhibitory G protein-calcium channel complex, resulting in enhanced calcium influx. Regulation of the inhibitory G protein-calcium channel complex involves PTX-sensitive and -insensitive G proteins.     

Inter-image outliers and their application to image classification

Image variability that is impossible or difficult to restore by intra-image processing, such as the variability caused by occlusions, significantly reduces the performance of image-recognition methods. To address this issue, we propose that the pixels associated with large distances obtained by inter-image pixel-by-pixels comparisons should be considered as inter-image outliers and should be removed from the similarity calculation used for the image classification. When this method is combined with the template-matching method for image recognition, it leads to state-of-the-art recognition performance: 91% with AR database that includes occluded face images, 90% with PUT database that includes pose variations of face images and 100% with EYale B database that includes images with large illumination variation.     

Using satellite data to extract volume–area–elevation relationships for Urmia Lake,Iran

Urmia Lake in the northwest of Iran is the second largest hyper-saline lake worldwide. During the past two decades, a significant water level decline has occurred in the lake. The existing estimations for the lake water balance are widely variable because the lake bathymetry is unknown. The main focus of this study is to extract the volume–area–elevation (V–A–L) characteristics of Urmia Lake utilizing remote sensing data and analytical models. V–A–L equations of the lake were determined using radar altimetry data and their concurrent satellite-derived surface data. Next, two approximate models, a power model (PM) and a truncated pyramid model (TPM), were parameterized for Urmia Lake and checked for accuracy. Results revealed that in comparison with the satellite-derived reference volume–elevation equation, the PM slightly over-predicts the volume of Urmia Lake while the TPM under-estimates the lake storage. Variations of the lake area and volume between 1965 and 2011 were examined using the developed V–A–L equations. Results indicated that the lake area and volume have declined from the historical maximum values by 2200 km² and 33 km³, respectively. To restore Urmia Lake to a level to maintain ecological benefits, 13.2 km³ of water is required. This study demonstrates the use of remote sensing data of different types to derive V–A–L equations of lakes. Substituting satellite-derived V–A–L equations for common empirical formulas leads to more accurate estimations of a lake water balance, which in turn, provides insight to water managers for properly assessing and allocating water resources to downstream ecosystems.     

Efficient biodegradation of naphthalene by a newly characterized indigenous Achromobacter sp. FBHYA2 isolated from Tehran Oil Refinery Complex

A bacterial strain, FBHYA2, capable of degrading naphthalene, was isolated from the American Petroleum Institute (API) separator of the Tehran Oil Refinery Complex (TORC). Strain FBHYA2 was identified as Achromobacter sp. based on physiological and biochemical characteristics and also phylogenetic similarity of 16S rRNA gene sequence. The optimal growth conditions for strain FBHYA2 were pH 6.0, 30 °C and 1.0% NaCl. Strain FBHYA2 can utilize naphthalene as the sole source of carbon and energy and was able to degrade naphthalene aerobically very fast, 48 h for 96% removal at 500 mg/L concentration. The physiological response of Achromobacter sp., FBHYA2 to several hydrophobic chemicals (aliphatic and aromatic hydrocarbons) was also investigated. No biosurfactant was detected during bacterial growth on any aliphatic/aromatic hydrocarbons. The results of hydrophobicity measurements showed no significant difference between naphthalene- and LB-grown cells. The capability of the strain FBHYA2 to degrade naphthalene completely and rapidly without the need to secrete biosurfactant may make it an ideal candidate to remediate polycyclic aromatic hydrocarbon (PAH)-contaminated sites.     

A two-dimensional warranty servicing strategy based on reduction in product failure intensity

The cost of servicing a warranty depends, amongst other factors, on the type of repair performed under warranty. Although “all minimal repair” and “all replacement” policies are easy to implement and analyze, they are not always feasible and/or practical. Having a combination of different types of repair often leads to lower warranty servicing costs. In this article, to reduce the warranty servicing cost, we study a servicing strategy that involves performing imperfect repairs in place of some of the minimal repairs of an “all minimal repair” strategy; the effect of an imperfect repair is characterized by a drop in the conditional intensity function of the failure process. We consider both fixed and random degrees of repair. For a given type of product, we partition the warranty region so that the expected total warranty servicing cost is minimized. We provide a numerical illustration and a comparison with previously-studied repair-replacement strategies.     

A blur-robust descriptor with applications to face recognition

Understanding the effect of blur is an important problem in unconstrained visual analysis. We address this problem in the context of image-based recognition by a fusion of image-formation models and differential geometric tools. First, we discuss the space spanned by blurred versions of an image and then, under certain assumptions, provide a differential geometric analysis of that space. More specifically, we create a subspace resulting from convolution of an image with a complete set of orthonormal basis functions of a prespecified maximum size (that can represent an arbitrary blur kernel within that size), and show that the corresponding subspaces created from a clean image and its blurred versions are equal under the ideal case of zero noise and some assumptions on the properties of blur kernels. We then study the practical utility of this subspace representation for the problem of direct recognition of blurred faces by viewing the subspaces as points on the Grassmann manifold and present methods to perform recognition for cases where the blur is both homogenous and spatially varying. We empirically analyze the effect of noise, as well as the presence of other facial variations between the gallery and probe images, and provide comparisons with existing approaches on standard data sets.     

刚性屋面渗漏的原因及预防措施

本文针对刚性防水屋面渗漏的原因进行了分析,并提出了处理措施,旨在控制刚性防水屋面渗漏的产 生,保证屋面质量,满足使用要求。     

Superscalar instruction issue

Clearly, instruction issue and execution are closely related: The more parallel the instruction execution, the higher the requirements for the parallelism of instruction issue. Thus, we see the continuous and harmonized increase of parallelism in instruction issue and execution. This article focuses on superscalar instruction issue, tracing the way parallel instruction execution and issue have increased performance. It also spans the design space of instruction issue, identifying important design aspects and available design choices. The article also demonstrates a concise way to represent the design space using DS trees, reviews the most frequently used issue schemes, and highlights trends for each design aspect of instruction issue     

New pseudo-dynamic analysis of two-layered cohesive-friction soil slope and its numerical validation

Natural slopes consist of non-homogeneous soil profiles with distinct characteristics from slopes made of homogeneous soil. In this study, the limit equilibrium modified pseudo-dynamic method is used to analyze the stability of two-layered c-φ soil slopes in which the failure surface is assumed to be a logarithmic spiral. The zero-stress boundary condition at the ground surface under the seismic loading condition is satisfied. New formulations derived from an analytical method are proposed for the predicting the seismic response in two-layered soil. A detailed parametric study was performed in which various parameters (seismic accelerations, damping, cohesion, and angle of internal friction) were varied. The results of the present method were compared with those in the available literature. The present analytical analysis was also verified against the finite element analysis results.     

老幼复合型社区养老机构的构想与实践——日本的经验与启示

作为社区型养老机构的特殊类型,将老年人和儿童看护设施邻近或合并设置的"老幼复合型机构"因其对促进不同世代人群间交流、理解的独特社会效益而在海外受到瞩目。日本从20世纪90年代起尝试建设此类机构,而对其使用情况的追踪调查也证实了设施对促进使用者身心健康以及社区人际交往的积极意义。本文认为尽管面临着国情和相关规范方面的诸多不同,但从社会和经济意义的角度考虑,老幼复合型机构仍然值得在中国试点推广。